Functional-material-based glucose biosensor and manufacturing method thereof

A technology of biosensor and glucose, which is applied in the field of biotechnology analysis and detection, can solve problems such as the interference of oxygen content changes, and achieve the effects of shortened response time, safe operation, and low-cost analysis

Inactive Publication Date: 2012-06-20
GUILIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent CN1219676A uses a disposable dual-electron dual-enzyme biosensor to detect cholesterol and glucose in the blo...

Method used

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  • Functional-material-based glucose biosensor and manufacturing method thereof
  • Functional-material-based glucose biosensor and manufacturing method thereof
  • Functional-material-based glucose biosensor and manufacturing method thereof

Examples

Experimental program
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Effect test

preparation Embodiment 1

[0056] Preparation Example 1 Graphene Preparation

[0057] Modified Hummers method combined with hydrazine hydrate reduction method: first, graphite (1.5 g, 325 mesh) was added to 12 ml concentrated H 2 SO 4 , 2.5 g K 2 S 2 o 8 and 2.5 g P 2 o 5 In the mixture, heat the above mixed system to 80 o C, maintain this temperature, and magnetically stir for 5 hours. Then the reaction system was cooled to room temperature, and the mixture was poured into 500 ml of deionized water and left to stand overnight. Filter the above standing matter through a 0.2 micron filter membrane, wash and dry naturally to obtain pre-oxidized graphite. Add the pre-oxidized graphite to 0 o Concentrated H of C 2 SO 4 120 ml, then slowly add 15 g KMnO 4 , and control the reaction temperature at 20 o c Stir. Potassium permanganate was added, and the reaction system was controlled at 35 o C was stirred for 4 hours, then, 250 ml of deionized water was added, and the temperature was controlled...

preparation Embodiment 2

[0058] Preparation Example 2 Preparation of Graphene Nanomaterials (Palladium Nanoparticles / Graphene Composite Pd NPs / CSGR)

[0059] First, disperse 200 mg of graphene oxide into a mixed medium of 40 ml thionyl chloride and 1 ml N,N-dimethylformamide, sonicate at room temperature for 0.5 hours, and then reflux for 52 hours to prepare 219.2 mg of brown acid chloride compound . At the same time, mix 1.753 g of phthaloyl chitosan), 1.201 g of LiCl and 120 ml of N, N-dimethylacetamide, and 140 o C was reacted for 2 hours. After cooling the reaction system, 219.2 mg of the brown acid chloride compound prepared in the previous step and 14 ml of pyridine were added to the reaction system, and refluxed for 48 hours under nitrogen protection. After cooling, it was filtered, washed, and vacuum-dried. After drying, the solid was stirred in 120 ml of distilled water for 6 hours, filtered, and the retained solid was redispersed in 200 ml of water, ultrasonicated for 1 hour, filtered, and...

preparation Embodiment 3

[0063] Preparation Example 3 Sensor Working Electrode Preparation

[0064]Grind the glassy carbon electrode with 1200# metallographic sandpaper first, and then use 1 μm, 0.3 μm, 0.05 μm Al 2 o 3 Polished, rinsed with distilled water, in 1:1 HNO 3 Ultrasonic cleaning with aqueous solution, absolute ethanol, and distilled water for 5 min each, and drying at room temperature for later use. Take 2 mg of the nanoparticle-functionalized graphene material prepared above, and disperse it in 1 ml of 5% acetic acid. After ultrasonication, take 8 μL of the above-mentioned dispersion solution and drop it on the surface of the above-mentioned glassy carbon electrode. After drying, the nanoparticle-functionalized graphene material is obtained. Graphene material modified electrodes. The electrode was soaked with 0.5% glutaraldehyde solution at room temperature, then washed with deionized water and dried at room temperature. Drop 8 μL of 4 mg / ml glucose oxidase (GOD) solution on the nan...

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Abstract

The invention relates to a functional-material-based glucose biosensor. A working electrode of the glucose biosensor is made from a graphene nanomaterial, the glucose biosensor is formed by using the working electrode made from the graphene nanomaterial in the assembling of the glucose biosensor, belongs to a quantitative test technology and shows excellent linearity and resolution, and the detection sensitivity, the detection range and the detection speed are increased; and particularly in the concentration range of human blood glucose, the amplitude of a response current can be increased by 50%, and the resolution can be increased by over twice. The functional-material-based glucose biosensor and a preparation method thereof have the advantages that: immobilized enzyme of a nano-functional membrane prepared from the graphene nanomaterial can be continuously analyzed for over 1,000 times, so that the cost for measurement is low, the accuracy of analysis is higher than that of other methods, the relative error reaches about 1%, the response time is shortened to 20 seconds, the service life is also greatly prolonged, the precise timing measurement of the concentration of glucose can be achieved; and the functional-material-based glucose biosensor has the characteristics of high specificity, short-time performance, low-cost analysis, no special requirements on analyzing substances, safety and simplicity and convenience in operation, and convenience for on-site measurement and the like and can be applied to the daily measurement of blood glucose of diabetes patients.

Description

technical field [0001] The invention relates to biotechnology analysis and detection, in particular to a biosensor, more specifically to a glucose biosensor based on a functional material and a preparation method thereof. Background technique [0002] A biosensor is an instrument that is sensitive to biological substances and converts its concentration into an electrical signal for detection. It is made of immobilized biosensitive materials as recognition elements (including enzymes, antibodies, antigens, microorganisms, cells, tissues, nucleic acids and other biologically active substances) and appropriate physical and chemical transducers (such as oxygen electrodes, photosensitive tubes, field effect tubes, Piezoelectric crystals, etc.) and an analysis tool or system composed of a signal amplification device. Biosensors have the functions of receptors and converters. [0003] Since the advent of the enzyme electrode in the 1960s, biosensors have undergone tremendous deve...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 程金生
Owner GUILIN MEDICAL UNIVERSITY
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