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Calcium alginate microcapsule, preparation method and application thereof

A technology of calcium alginate and microcapsules, which is applied in the direction of microcapsule preparation, microsphere preparation, microcapsules, etc., can solve the problems of high cost, complicated steps, unfavorable mass production, etc., achieve uniform shape, simple preparation method, and The effect of short process time

Active Publication Date: 2012-08-08
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also literature reports using liposomes as templates to load sodium alginate into liposomes, control the penetration of calcium ions by temperature to prepare calcium alginate capsules, and then use OBG to remove the phospholipid bilayer, but this method The steps are more complicated, which is not conducive to mass production, and the cost is higher (seeing Langmuir2008, 24, 4092-4096, Liposome-Templated Supramolecular Assembly of Responsive Alginate Nanogels)

Method used

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  • Calcium alginate microcapsule, preparation method and application thereof
  • Calcium alginate microcapsule, preparation method and application thereof
  • Calcium alginate microcapsule, preparation method and application thereof

Examples

Experimental program
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Embodiment 1

[0042] Embodiment 1, preparation calcium alginate microcapsule

[0043] Add 0.5mL 4mg / mL sodium alginate solution to 1mL 0.33mol / L sodium carbonate solution, stir evenly with magnetic force, then add 1mL 0.33mol / L calcium chloride solution, stir for 30s, centrifuge at 3000rpm for 3min, remove For excess ions, wash 3 times. Then slowly add 0.01M dilute hydrochloric acid solution to the washed suspension to remove the template and form calcium alginate gel microcapsules at the same time. Gel microcapsules with a diameter of about 3 μm (such as figure 1 shown). Photoelectron spectroscopy (such as figure 2 The appearance of calcium ions in ) confirmed that the capsules we prepared were formed by cross-linking calcium ions with sodium alginate. The ratio between calcium ions and guluronic acid groups is 1:2, and it is reported in the literature that when the ratio of the two is 1:4, a stable dimer structure can be formed, increasing the ratio between the two can form a multim...

Embodiment 2

[0044] Embodiment 2, preparation calcium alginate microcapsule

[0045] Doxorubicin was selected as the model drug to make the drug release curve, and the characteristic ultraviolet absorption peak was at 480nm.

[0046] Prepare a 1mg / mL doxorubicin solution. Calcium alginate gel capsules prepared according to the method and dosage of Example 1 were counted as 1 part and added to 1 mL of doxorubicin solution for adsorption for 5 hours and then washed 3 times at 3500 rpm. Due to the adsorption between doxorubicin and calcium alginate, it can stably exist in the compact structure of calcium alginate in aqueous solution without being released. Laser confocal images such as image 3 As shown in A, using a light source with a wavelength of 488nm to excite doxorubicin (green) confirms the integrity and cavity structure of the calcium alginate microcapsules loaded with doxorubicin. The drug release curve was made in PBS (pH=7.4) solution at 37°C, and the change of the absorption p...

Embodiment 3

[0048] Prepare a 1mg / mL doxorubicin solution.

[0049] Calcium alginate gel capsules prepared according to the method and dosage of Example 1 were counted as 1 part and added to 1mL doxorubicin solution for adsorption for 5 hours and then washed 3 times at 3500rpm to obtain calcium alginate microcapsules loaded with doxorubicin 1 serving.

[0050] Due to the three-dimensional network structure of the gel, which has a large pore size, it is likely to cause the rapid release of small molecule drugs in the release experiment, so we modified its surface. Firstly, 0.6-2 mg / mL liposomes are prepared, and the liposomes can be positively charged or uncharged phospholipids. In this example, dimyristoylphosphatidylcholine (DMPC) was selected to prepare liposomes with a diameter of about 200 nm.

[0051] The preparation method of liposome is as follows:

[0052] First, a certain amount of dimyristoylphosphatidylcholine (DMPC) was weighed and dissolved in a mixed solution of chloroform...

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Abstract

The invention discloses a calcium alginate microcapsule and a preparation method thereof. The calcium alginate microcapsule is prepared according to the method which comprises the following steps: 1) uniformly mixing a sodium alginate solution with a soluble carbonate solution, thereby obtaining a mixed solution; 2) adding a soluble calcium salt solution into the mixed solution, and mixing uniformly, thereby obtaining an emulsion; and 3) adding an acid solution into the emulsion, enabling calcium ions generated by dissolving calcium carbonate microspheres to crosslink with sodium alginate, thereby forming the calcium alginate microcapsule. The preparation method is simple and is easily performed; the yield of product is high; the size distribution is uniform; and the size is controllable. The microcapsule is a single-component system; the introduction of irrelevant matters is largely reduced, the application range of a carrier is increased and the toxic side effect is reduced. Besides, phospholipid is used for functionalizing the calcium alginate microcapsule, a novel drug carrier is created, and a new more effective way is supplied for combined treatment.

Description

technical field [0001] The invention relates to a calcium alginate capsule, a preparation method and application thereof. Background technique [0002] Worldwide, malignant tumors are always the leading cause of death for humans. There are many methods currently used to treat tumors, including surgery, chemotherapy, radiotherapy, hyperthermia and photodynamic therapy. Photodynamic therapy is a cold photochemical reaction. Oxygen, photosensitizer and visible light are the basic conditions for the photodynamic effect to occur. The photosensitizer itself is non-toxic, but after being irradiated by light of the corresponding wavelength, it can react with oxygen to produce highly active singlet oxygen, which will oxidize with adjacent biomacromolecules, resulting in cytotoxicity and cell damage structure or affect cell function, leading to cell damage and even death, thereby destroying cancer cells. In recent years, due to the development and progress of photosensitive substan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K47/36A61K31/704A61K51/00A61P35/00C08J3/24B01J13/14
Inventor 李峻柏杜翠玲赵洁
Owner INST OF CHEM CHINESE ACAD OF SCI