Method for preparing sulfated silk fibroin vascular tissue engineering scaffold by using electrostatic spinning

Abstract

The invention relates to a sulfated silk fibroin vascular tissue engineering scaffold prepared by using electrostatic spinning and belongs to the field of biological medical materials. The sulfated silk fibroin vascular tissue engineering scaffold is prepared through the following steps of: dissolving a silk fibroin and sulfated silk fibroin sponge in hexafluoroisopropanol, so as to prepare a 7-10% mixed silk fibroin and sulfated silk fibroin solution; filling the solution in an injector, applying a 10-30kV voltage, and receiving fibers by using a rotary metal rod, wherein the receiving distance is 10-25 cm, the diameter of the selected receiving rod is 3-5 mm, the rotating speed is 200-1000 rpm (revolutions per minute), and the propelling speed of the injector is 0.03-0.05 mm / s, so as to obtain a tubular tissue engineering scaffold with the thickness of 0.1-1 mm and the internal diameter of 3-5 mm; then inducing the silk fibroin to convert to a beta laminated structure by using a 90-100% methanol solution to soak the scaffold, so that the tissue engineering scaffold can be prevented from being dissolved in water by virtue of improving mechanical properties. The vascular tissue engineering scaffold prepared by the invention has excellent cell compatibility and long-term and stable anti-blood coagulation property, has controllable caliber, and can be used for maintenance of small-caliber blood vessels and establishment of tissue engineering and hemodialysis access. The preparation method provided by the invention has the advantages of simplicity in operation and low cost, and can be used for realizing commercial production.

Description

(1) Technical field [0001] The invention belongs to the field of biomedical materials, in particular to a sulfated silk fibroin vascular tissue engineering scaffold with good anticoagulant performance and a preparation method thereof. (2) Background technology [0002] Cardiovascular diseases seriously endanger human health, and vascular reconstruction plays a very important role in clinical surgery. More than 600,000 people around the world need to undergo various vascular surgeries every year, and most of them need suitable vascular grafts. At present, the vascular grafts used in clinical practice mainly include autologous blood vessels, allogeneic blood vessels and artificial synthetic blood vessels. Due to the limited source of autologous blood vessels and the rejection of allogeneic blood vessels, artificial vascular grafts have attracted much attention. At present, large-caliber artificial blood vessels with a diameter of more than 6 mm have been commercialized, while ...

AI Technical Summary

Problems solved by technology

Heparin is a common anticoagulant protein. Heparin can improve hemorheological properties after simple coating, but the binding strength is not enough, and it is easy to fall off, resulting in too fast release

In recent years, some scholars have used plasma irradiation to change the surface structure of materials, generate reactive groups on the surface of materials, and fix heparin on the surface of materials in the form of covalent bonds, but the anticoagulant effect is not satisfactory.

The scaffold material in the in vivo environment is exposed to various factors such as body fluids, organic macromolecules, enzymes, free radicals, cells, etc., and its biological environment is extremely complex. The method of surface modification can improve the long-term anticoagulant performance of the material Relatively limited, with the degradation of the stent material and the shedding of the surface modification layer, although its anticoagulant performance can meet the needs of large-caliber vessels with high blood flow and low resistance, it still cannot meet the requirements of small-caliber vessels with high anticoagulant performance. blood vessel needs

On the other hand, planting vascular endothelial cells on the inner surface of the scaffold material can form an anti-thrombotic surface layer, which can reduce the activity of prothrombin on the inner surface of tissue-engineered blood vessels, thereby improving the hemocompatibility of the material, especially improving the stability of small-diameter tissue-engineered blood vessels. However, the insufficient preservation rate of endothelial cells after being washed by blood flow has always been a major problem for domestic and foreign scholars.