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Biological antibacterial agent, as well as preparation method and application thereof

A biological antibacterial agent, lactic acid bacteria technology, applied in the directions of botanical equipment and methods, biocides, chemicals for biological control, etc. The effect of reducing preparation cost, improving antibacterial activity and reducing side effects

Active Publication Date: 2012-10-03
CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although there have been a certain amount of reports on the use of various nanoparticles for in vivo localized therapy, few reports have delved into how to improve the efficiency of these in vivo antibacterial agents.
Although many nano-antibacterial agents can be accurately fixed in designated areas, they cannot be effectively dispersed, so it is difficult to fully contact with pathogenic bacteria. In this case, in order to ensure antibacterial efficiency, the input amount of nano-antibacterial agents can only be increased
In this way, it not only increases the pain and expense of the patient, but also an additional burden on the metabolic system

Method used

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  • Biological antibacterial agent, as well as preparation method and application thereof
  • Biological antibacterial agent, as well as preparation method and application thereof
  • Biological antibacterial agent, as well as preparation method and application thereof

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preparation example Construction

[0040] The preparation method of biological antibacterial agent of the present invention, comprises the steps:

[0041] 1. Under nitrogen protection, add ammonia water to Fe 2+ with Fe 3+ In the mixed solution, make the pH value of the solution ≧10, stir rapidly at 85°C, and continue to stir for 100 minutes after the color of the reaction solution becomes darker, and then magnetically collect the black Fe in the solution under the action of an external magnetic field 3 o 4 Precipitated, washed with distilled water until neutral, dried to obtain magnetic Fe 3 o 4 nanoparticles;

[0042] 2, the magnetic Fe obtained in step 1 3 o 4 Mix nanoparticles with water-in-oil (W / O) microemulsion containing Triton X-100, n-hexanol and cyclohexane, then add silver nitrate solution to react for 30 minutes, add sodium borohydride solution and stir at room temperature for 3-8 hours, then add excess acetone to precipitate the product in the solution, after centrifugation, wash repeatedly...

Embodiment 1

[0046] Embodiment 1: the preparation of biological antibacterial agent of the present invention

[0047] Take 43.10mL1.00mol / LFeCl 2 4H 2 O solution and 43.10mL 1.75mol / LFeCl 3 ·6H 2 O solution was mixed, under the protection of nitrogen, 25mL of 25% ammonia water was added, and stirred rapidly at 85°C, the color of the reaction solution became darker, and brown particles were formed, and the stirring was continued for 100 minutes. Use a strong magnet to settle the particles and separate the supernatant, and wash the precipitate repeatedly with distilled water until the pH of the washed solution is about 7. Collect the precipitate and place it in a vacuum drying oven, and dry it in vacuum at 75°C for 5 hours to obtain magnetic Fe 3 o 4 nanoparticles.

[0048] Add 1 mL of magnetic Fe at a concentration of 40 mM 3 o 4 Nanoparticles were mixed with a water-in-oil (W / O) microemulsion containing 14mL Triton X-100, 14mL n-hexanol and 75mL cyclohexane, and then added 5mL of 0...

Embodiment 2

[0052] Embodiment 2: dispersibility and antibacterial property experiment of biological antibacterial agent of the present invention

[0053] In order to prove the potential superiority of the biological antibacterial agent of the present invention for targeted antibacterial in vivo. A mixed aqueous solution (pH=7.1, 37°C) containing beef peptone (5g / L), glucose (5g / L), agar (20g / L) and multivitamin (10mg / L) was selected to simulate the complex and viscous liquid in the human body. In a liquid environment, the ability of the biological antibacterial agent of the present invention to treat four common pathogenic bacteria (Escherichia coli, Staphylococcus aureus, Helicobacter pylori and Bacillus subtilis) was investigated under this condition. In every 30mL of bacterium-containing solution, add the biological antibacterial agent that the embodiment 1 of 5.4mg makes (wherein, effective antibacterial composition Ag / Fe 3 o 4 The content of composite nanoparticles is about 0.6 mg)...

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Abstract

The invention belongs to the field of biological materials, and discloses a biological antibacterial agent, as well as a preparation method and application thereof. The biological antibacterial agent consists of lactic acid bacteria, Ag nano-particles and magnetic Fe3O4 nano-particles, wherein the Ag nano-particles wrap the surfaces of the magnetic Fe3O4 nano-particles to form Ag and Fe3O4 composite nano-particles; and the Ag / Fe3O4 composite nano-particles are loaded on the surface of the lactic acid bacteria through hydrogen bonds. The antibacterial activity can be promoted by wrapping the Ag nano-particles on the surfaces of the magnetic Fe3O4 composite particles, the magnetic Ag / Fe3O4 composite nano-particles loaded on lactic acid bacteria can float in pathogenic bacteria-containing solution for a long time without extra dispersing treatment by virtue of low density of the lactic acid bacteria, so that the magnetic Ag / Fe3O4 composite nano-particles can be sufficiently contacted with the pathogenic bacteria floating in the solution, and can be accurately immobilized in a designated area and kept in a dispersing state for a long time in the body, so that the bacteriostasis and antibiosis capabilities of the biological antibacterial agent can be sufficiently played.

Description

technical field [0001] The invention belongs to the field of biological materials, in particular to a biological antibacterial agent and its preparation method and application. Background technique [0002] Antibiotics are a class of secondary metabolites with anti-pathogen or other activities produced by microorganisms or higher animals and plants in their living metabolism. They are a class of chemical substances that can kill, inhibit or interfere with other microorganisms at very low concentrations. At the same time, antibiotics are also a special class of chemical agents. Since the discovery of the first antibiotic penicillin in 1929, about 10,000 new antibiotics have been found so far. my country is a big country in the use of antibiotics. According to the 2006-2007 National Bacterial Resistance Surveillance Results of the Ministry of Health, the annual use rate of antibacterial drugs in hospitals in my country is as high as 74%. In developed countries such as the Uni...

Claims

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Application Information

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IPC IPC(8): A61K33/38A61K47/02A61K47/12A61K47/48A01N59/16A01P3/00A61P31/04A61K31/19
CPCY02A50/30
Inventor 杨秀荣王小磊
Owner CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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