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A strain preservation culture solution for efficiently degrading lignocellulose and its preparation method

A technology of lignocellulose and culture fluid, applied in the direction of adding compounds to stimulate growth, etc., can solve problems such as easy to be decomposed

Inactive Publication Date: 2015-10-28
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the fiber is swollen, it is easily broken down

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Add 1L deionized water to a 2L sterilized clean container, then add 1.35g magnesium sulfate, 0.25g ferric sulfate, 0.90g ammonium nitrate, 1.60g glutamine, 0.20g sodium molybdate, 0.033g cobalt chloride, 1.40g Potassium Iodide, 1.25g Potassium Dihydrogen Phosphate, 4.50g Glucose, 0.20g Sodium Sulfur Glycolate, 1.45g Citric Acid and 0.09g Asparagine, stir until all substances are completely dissolved, heat to 28°C and add 1.09g yeast powder , 3.50g starch, 1.45g beef extract and 3.57g peach gum powder were fully mixed and placed in a constant temperature incubator for fermentation and preservation for 3 days to obtain the strain preservation culture solution.

Embodiment 2

[0016] Add 1L deionized water to a 2L sterilized clean container, then add 3.12g magnesium sulfate, 0.23g ferric sulfate, 0.95g ammonium nitrate, 1.50g glutamine, 0.30g sodium molybdate, 0.023g cobalt chloride, 1.36g Potassium Iodide, 1.56g Potassium Dihydrogen Phosphate, 5.00g Glucose, 0.22g Sodium Sulfur Glycolate, 1.55g Citric Acid and 0.10g Asparagine, stir until all substances are completely dissolved, heat to 35°C and add 2.15g yeast powder , 3.55g starch, 1.71g beef extract and 3.67g peach gum powder were fully mixed and placed in a constant temperature incubator for fermentation and preservation for 5 days to obtain the culture medium for bacterial strain preservation.

Embodiment 3

[0018] Add 1L deionized water to a 2L sterilized clean container, then add 1.40g magnesium sulfate, 1.57g ferric sulfate, 0.83g ammonium nitrate, 1.80g glutamine, 0.18g sodium molybdate, 0.082g cobalt chloride, 1.45g Potassium Iodide, 1.22g Potassium Dihydrogen Phosphate, 8.32g Glucose, 0.18g Sodium Sulfur Glycolate, 2.38g Citric Acid and 0.11g Asparagine, stir until all substances are completely dissolved, heat to 29°C and add 1.15g yeast powder , 4.00g starch, 1.43g beef extract and 7.50g peach gum powder were fully mixed and placed in a constant temperature incubator for fermentation and preservation for 4 days to obtain the strain preservation culture solution.

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Abstract

The invention relates to a preservation culture solution of bacterial strain for efficiently degrading lignocellulose and a preparation method of the culture solution, belonging to the field of biotechnology. The culturing solution provided by the invention is characterized in that each 1L of deionized water contains the following materials: magnesium sulfate, ferric sulfate, ammonium nitrate, glutamine, sodium molybdate, cobalt chloride, potassium iodide, monopotassium phosphate, yeast powder, glucose, sulfuretted sodium glycollate, citric acid, starch, beef extract, asparagine and peach gum. The culturing solution is added with multiple nutrient elements which can promote the bacterial strain for degrading lignocellulose to rapidly and stably grow; the culturing solution is simple in preparation method, low in equipment investment, low in material cost and can accurately, stably and efficiently cultivate excellent bacterial strains with good adaptability; and the lignocellulose degrading activity of the obtained bacterial strain is higher than that of the bacterial strain naturally grown in natural world.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain preservation culture solution for efficiently degrading lignocellulose. Background technique [0002] Lignocellulosic resources are the most abundant and cheap renewable resources on earth, and lignocellulose can be converted into three main utilization modes: transportation fuel, thermal or electrical energy, and chemical feedstock. The utilization of cellulosic biomass will be one of the important ways for human beings to solve food and energy problems in the future. For a long time, scholars at home and abroad have been committed to finding the best way to degrade plant lignocellulose. The research generally focuses on the following aspects: physical and chemical treatment, such as hot water treatment, steam explosion, low-temperature ammonia explosion treatment, dilute acid treatment, alkali treatment , wet oxygen treatment, organic solvent treatment, superc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38
Inventor 林海萍周湘毛胜凤
Owner ZHEJIANG FORESTRY UNIVERSITY
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