Method utilizing HPLC to test seventeen phenol substances contained in grapes and oranges
A technology for phenolic substances and determination methods, applied in the field of analytical chemistry, can solve the problem of difficulty in detecting the real content, and achieve the effect of simple sample pretreatment and low cost
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0022] Example 1 Determination of phenolic substances in grapes
[0023] 1 Standard sample: gallic acid, catechin, epicatechin, chlorogenic acid, caffeic acid, syringic acid, coumaric acid, rutin, ferulic acid, benzoic acid, hesperidin, naringin, salicylic acid Acid, quercetin, resveratrol, tangeretin, and orange peel were purchased from sigma company.
[0024] 2 sample preparation
[0025] 2~3g freeze-dried fruit sample (wine) → 50% ethanol grinding → ethyl acetate extraction twice → collect the extract → concentrate under reduced pressure to dryness → dissolve in 2ml solvent (methanol:DMSO=1:1).
[0026] 3 Chromatographic conditions
[0027] Column Merck LiChrospher_100RP18e (250mm×4.0mm); detection wavelength: 0-20min: 230nm; 20-35min: 280nm; 35-42min: 309nm; 42-60min: 320nm; column temperature: 30℃; gradient elution: mobile phase A is water: acetic acid = 98: 2; mobile phase B is acetonitrile. Elution procedure: 0min→100min, acetonitrile: 16%→70%, 2% acetic acid water mixture: 84...
Example Embodiment
[0030] Example 2 Citrus peel and pulp
[0031] 1 Standard sample: gallic acid, catechin, epicatechin, chlorogenic acid, caffeic acid, syringic acid, coumaric acid, rutin, ferulic acid, benzoic acid, hesperidin, naringin, salicylic acid Acid, quercetin, resveratrol, tangeretin and orange peel were purchased from sigma company.
[0032] 2 Sample preparation
[0033] Separate the pulp and skin of the sample and freeze-dry 60 meshes for use; mix 0.1g sample with 1mL methanol-DMSO (V / V.50:50) for 10min at room temperature, 9000rpm, 15min at 4℃, repeat 3 times, combine the supernatant In a volumetric flask, make the volume constant with methanol; filter with a 0.45μm microporous membrane for use.
[0034] 3 Chromatographic conditions
[0035] Column Merck LiChrospher_100RP18e (250mm×4.0mm); detection wavelength: 0-20min: 230nm; 20-35min: 280nm; 35-42min: 309nm; 42-60min: 280nm; column temperature: 30℃; gradient elution: mobile phase A is water: acetic acid = 98: 2; mobile phase B is aceton...
Example Embodiment
[0038] Example 3 Extraction of phenolic substances in wine samples
[0039] 1 Standard sample: gallic acid, catechin, epicatechin, chlorogenic acid, caffeic acid, syringic acid, coumaric acid, rutin, ferulic acid, benzoic acid, hesperidin, naringin, salicylic acid Acid, quercetin, resveratrol, tangeretin and orange peel were purchased from sigma company.
[0040] 2 sample preparation
[0041] Sampling and determination of monomeric phenols in wine. The extraction method is similar to that of grape fruit. The process route is: wine (10 ml) → 50% ethanol extraction twice (5 times the material-to-liquid ratio, 70°C for 60 min) → reduced pressure concentration → Trichloromethane extraction 2 times →
[0042] Extract with ethyl acetate twice → concentrate under reduced pressure → extract the extract → dissolve in methanol, dilute to 10 ml → store at 4°C for use, and repeat 3 times.
[0043] 3 Chromatographic conditions
[0044] Column Merck LiChrospher_100RP18e (250mm×4.0mm); detection wav...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap