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Tissue culture medium for propagating anthurium buds by using buds

A technology of tissue culture and culture medium, which is applied in horticulture, botany equipment and methods, gardening tools/equipment, etc., can solve the problems that affect the excellent genetic traits of seedlings, and are prone to variation, so as to be beneficial to preservation, production and utilization, Neat growth, guaranteed quality and quality effect

Inactive Publication Date: 2012-12-19
钦州市林业科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research reports on Anthurium tissue culture at home and abroad all use leaves, petioles, stems and other organs to induce callus, and then induce callus to differentiate into adventitious buds. The adventitious buds obtained by this way are in the process of proliferation It is prone to variation, which affects the original excellent genetic traits of the seedlings

Method used

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  • Tissue culture medium for propagating anthurium buds by using buds

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Anthurium anthurium bud propagation tissue culture medium, the medium includes subculture medium and rooting medium, wherein:

[0060] The components and the content of each component per liter (L) of the subculture medium are as follows:

[0061] (1) Macroelements: potassium nitrate 1800mg / L, ammonium nitrate 1350mg / L, calcium chloride dihydrate 350mg / L, magnesium sulfate heptahydrate 320mg / L, potassium dihydrogen phosphate 150mg / L, ferrous sulfate heptahydrate 27.5mg / L, disodium edetate 37.3mg / L;

[0062] (2) Trace elements: manganese sulfate tetrahydrate 21.5mg / L, zinc sulfate heptahydrate 8.3mg / L, boric acid 6.0mg / L, sodium molybdate dihydrate 0.2mg / L, potassium iodide 0.83mg / L, copper sulfate pentahydrate 0.02mg / L, cobalt chloride hexahydrate 0.02mg / L;

[0063] (3) Organic matter: inositol 80.0mg / L, glycine 2.5mg / L, riboflavin (VB 2 ) 6.0mg / L, pyridoxine hydrochloride (VB 6 ) 0.45mg / L, niacin 0.45mg / L, thiamine hydrochloride (VB 1 ) 0.1mg / L;

[0064] (4) Pla...

Embodiment 2

[0073] Anthurium anthurium bud propagation tissue culture medium, the medium includes subculture medium and rooting medium, wherein:

[0074] The components and the content of each component per liter (L) of the subculture medium are as follows:

[0075] (1) Macroelements: potassium nitrate 1800mg / L, ammonium nitrate 1350mg / L, calcium chloride dihydrate 350mg / L, magnesium sulfate heptahydrate 320mg / L, potassium dihydrogen phosphate 150mg / L, ferrous sulfate heptahydrate 27.5mg / L, disodium edetate 37.3mg / L;

[0076] (2) Trace elements: manganese sulfate tetrahydrate 21.5mg / L, zinc sulfate heptahydrate 8.3mg / L, boric acid 6.0mg / L, sodium molybdate dihydrate 0.2mg / L, potassium iodide 0.83mg / L, copper sulfate pentahydrate 0.02mg / L, cobalt chloride hexahydrate 0.02mg / L;

[0077] (3) Organic matter: inositol 80.0mg / L, glycine 2.5mg / L, riboflavin (VB 2 ) 6.0mg / L, pyridoxine hydrochloride (VB 6 ) 0.45mg / L, niacin 0.45mg / L, thiamine hydrochloride (VB 1 ) 0.1mg / L;

[0078] (4) Pla...

Embodiment 3

[0087] Anthurium anthurium bud propagation tissue culture medium, the medium includes subculture medium and rooting medium, wherein:

[0088] The components and the content of each component per liter (L) of the subculture medium are as follows:

[0089] (1) Macroelements: potassium nitrate 1800mg / L, ammonium nitrate 1350mg / L, calcium chloride dihydrate 350mg / L, magnesium sulfate heptahydrate 320mg / L, potassium dihydrogen phosphate 150mg / L, ferrous sulfate heptahydrate 27.5mg / L, disodium edetate 37.3mg / L;

[0090] (2) Trace elements: manganese sulfate tetrahydrate 21.5mg / L, zinc sulfate heptahydrate 8.3mg / L, boric acid 6.0mg / L, sodium molybdate dihydrate 0.2mg / L, potassium iodide 0.83mg / L, copper sulfate pentahydrate 0.02mg / L, cobalt chloride hexahydrate 0.02mg / L;

[0091] (3) Organic matter: inositol 80.0mg / L, glycine 2.5mg / L, riboflavin (VB 2 ) 6.0mg / L, pyridoxine hydrochloride (VB 6 ) 0.45mg / L, niacin 0.45mg / L, thiamine hydrochloride (VB 1 ) 0.1mg / L;

[0092] (4) Pla...

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Abstract

The invention discloses a tissue culture medium for propagating anthurium buds by using buds. The culture medium comprises a subculture medium and a rooting medium. The tissue culture medium adopts direct induction of germ-free buds and proliferation and propagation instead of callus dedifferentiation for the first time. Compared with the callus dedifferentiation, the technique can be used for fast and easily obtaining germ-free buds, can avoid variation occurred during subculture proliferation, guarantees the grade and quality of seedlings, and is beneficial to storage and production utilization of germplasm resources.

Description

technical field [0001] The invention relates to a tissue culture medium, in particular to a tissue culture medium for anthurium bud propagation. Background technique [0002] Anthurium, also known as anthurium, anthurium, etc., belongs to the genus Anthurium in the family Araceae. It is native to the tropical rain forests of South America and is now widely cultivated in Europe, Asia, and Africa. The characteristic is that it likes warmth and fears cold, likes humidity and fears drought, and likes shade and avoids sun. Its flowers are unique, with Buddha flame inflorescences, bright and gorgeous colors, rich colors, leaf-shaped bracts, common bract colors are red, pink, white, etc., which have great ornamental value. It can be propagated by sowing and division. The flower language of Anthurium is great ambition, enthusiasm and blood. [0003] Anthurium is an important tropical cut flower, the inflorescence of the Buddha flame, the buds of the Buddha flame are huge, thick a...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 何贵整陈丽文时群吴红英陈乃明张树明王华宇赖崇健杨利平荣薏巫明忠
Owner 钦州市林业科学研究所
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