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Strains with high cellulase activity as well as screening method and use method of strains

A cellulase and activity technology, which is applied to high-yielding cellulase activity strains and the fields of screening and use thereof, can solve the problems of high cellulase price, high production cost, difficult operation and the like, and achieves low price and low production cost. , Easy to operate effect

Active Publication Date: 2014-07-16
GUIZHOU MOUTAI WINERY GRP XIJIU CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of chemical treatment methods generally requires high temperature and high pressure. Not only the production cost is high, but also the process is cumbersome and difficult to operate. Biological methods can solve this problem very well, but the price of cellulase is relatively expensive.

Method used

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  • Strains with high cellulase activity as well as screening method and use method of strains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Embodiment 1: The raw material used in this example is Daqu fermented for 31 days in January 2010. Weigh 10g of the Daqu sample, place it in a 250mL Erlenmeyer flask filled with small glass beads, add 90mL of sterile water and shake at 120r / min for 10min. Take 1mL of the suspension and add it to the Erlenmeyer flask of the cellulose inorganic salt liquid medium, and culture it statically at 28°C. After a large number of bacteria grow, inoculate 10% into the inorganic salt medium equipped with filter paper strips, and let it stand for several days at 28°C. After a large number of bacteria grow, transfer it to fresh culture medium and inoculate The amount is 10%, so that several generations are transferred to eliminate the cultures that lose their ability to decompose and are unstable. Observe the breakage of the filter paper strip, pick the culture at the breakage of the filter paper strip, streak and separate on the carboxymethyl cellulose medium plate, and culture at ...

Embodiment 2

[0082] Example 2: Utilize the strain xqx-6 screened in Example 1 to use liquor discarded grains as a carbon source to ferment and produce alcohol. Put 55g of dry liquor distilled grains, 27g of bran, 18g of ammonium sulfate, 500mL of water into a 1000mL conical flask, and cook at 121°C for 20min. After cooling, add 25mL of xqx-6 seed solution and 25mL of angel yeast seed solution, and culture at 30°C for 15 days. Its alcohol yield is 5%.

Embodiment 3

[0083] Example 3: The strain xqx-6 screened in Example 1 was used to ferment and produce alcohol with distiller's grains as a carbon source. Put 500g of wet liquor distilled grains into a 1000mL conical flask, 50mL of water, and cook at 121°C for 20min. After cooling, add 20 mL of xqx-6 seed liquid and 20 mL of Angel yeast seed liquid, and culture at 28°C for 20 days. Its alcohol yield is 3%.

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Abstract

The invention relates to the field of wine making, and particularly relates to strains with high cellulase activity as well as a screening method and a use method of the strains. The strains with high cellulase activity are aspergillus. The screening method comprises the following steps of: A. enriching and cultivating bacteria through an inorganic salt culture medium; B. carrying out once or multiple-time inoculation on the bacteria enriched and cultivated on filter paper dipped with the inorganic salt culture medium; C. acquiring a cultured product at the fracture part of the filter paper, and carrying out once or multiple-time streaking on the cultured product on a carboxymethylcellulose culture medium plate, so as to obtain pure strains; D. cultivating the pure strains obtained from the step C in the inorganic salt culture medium and carrying out fibrinolysis on the cultivated pure strains; E. inoculating the pure strains with high fibrinolysis capacity in the step D to a steriled solid fermentation medium for cultivating; and F. extracting coarse enzyme liquid by adopting a water bath method, and carrying out enzyme activity test to obtain the strains with high cellulase activity. The strains and bread yeast are compounded for use and fermented by taking wine waste lees and brans as main materials. With the adoption of the strains with high cellulase activity, the fermentation rate of the wine waste lees can be improved.

Description

technical field [0001] The present invention relates to the field of brewing, in particular to a strain with high cellulase activity, xqx-6, Aspergillus fumigatus is preserved in the China Center for Type Culture Collection (CCTCC), the address is Wuhan University, the zip code is 430072, and the preservation number is CCTCC NO.M 2012231, and its screening method and application method. Background technique [0002] Cellulase is a complex enzyme mainly composed of exo-β-glucanase, endo-β-glucanase and β-glucosidase, which can decompose cellulose into polysaccharides or monosaccharides. Cellulase has huge market potential in the fields of feed, alcohol, textile and food, and has been favored by insiders at home and abroad. It will be the fourth largest industrial enzyme after glucoamylase, amylase and protease. Therefore, cellulase It is a new growth point in the enzyme preparation industry. [0003] Cellulase is widely found in organisms in nature. Cellulase can be produc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/02C12Q1/04C12P39/00C12P7/10C12R1/68C12R1/865
CPCY02E50/16Y02E50/10
Inventor 钟方达胡峰唐云容李波杨开梅张文学赵盈盈吴正云钟霞
Owner GUIZHOU MOUTAI WINERY GRP XIJIU CO LTD
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