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Biological agent for processing industrial sewage and preparation method and application

A biological bacterial agent and bacterial agent technology, applied in the field of microorganisms, can solve problems such as complex components of industrial sewage, hazards to human physical and mental health, and inability to remove pollutants, and achieve the effects of simple preparation methods, good degradation effects, and improved biological treatment efficiency

Inactive Publication Date: 2013-01-23
何颖霞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The composition of industrial sewage is more complex, especially a large number of artificially synthesized compounds entering the environment, such substances are mainly alkanes, olefins, alicyclic hydrocarbons, aromatic hydrocarbons, and artificially synthesized organic substances, especially macromolecules, refractory, toxic Harmful substances such as phenol, chlorophenol, cresol, nitrophenol, etc., aromatic hydrocarbons, cyanides, amines, and ammonia nitrogen enter the environment with the wastewater or solid waste discharged from the factory. Due to the complexity of the structure of these substances and the biological The unfamiliarity of these pollutants cannot be decomposed and utilized by microorganisms in a short period of time. The traditional wastewater treatment method using activated sludge to cultivate domesticated microorganisms can no longer effectively remove these pollutants. These substances have accumulated in the environment for a long time, giving us The living ecological environment has caused great pollution, which has brought great harm to human physical and mental health.
A considerable number of industrial polluting enterprises in our country would rather be punished than invest in wastewater treatment. Even if there is a sewage treatment plant, the operation is extremely abnormal.

Method used

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  • Biological agent for processing industrial sewage and preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kind of composite bacterial agent for sewage treatment, the active ingredient of this bacterial agent comprises the raw material of following percentage by weight:

[0034] Rhodococcus 32%, Bacillus subtilis 20%, Pseudomonas aeruginosa 10%, Saccharomyces cerevisiae 10%,

[0035] Nitrosogenic bacteria 10%, Bacillus megaterium 8%, Aspergillus niger 5%, Paracoccus denitrification 5%.

[0036] Among the above bacteria agents:

[0037] The Rhodococcus is Rhodococcus (Rhodococcus rhodochrous) ATCC15906;

[0038] The Bacillus subtilis is Bacillus subtilis CGMCC No: 0954;

[0039] The Pseudomonas aeruginosa is Pseudomonas aeruginosa ATCC15442;

[0040] The Saccharomyces cerevisiae is Saccharomyces cerevisiae CCTCC No: M208110;

[0041] The nitrosogenic bacteria are Nitrosomonas europaea CCTCC No: M2010002;

[0042] The Bacillus megatherium is Bacillus megatherium (Bacillus megatherium) CGMCC No: 2267;

[0043] The Aspergillus niger is Aspergillus nige (Aspergillus nige)...

Embodiment 2

[0057] A kind of composite bacterial agent for sewage treatment, the active ingredient of this bacterial agent comprises the raw material of following percentage by weight:

[0058] Rhodococcus 32%, Bacillus subtilis 20%, Pseudomonas aeruginosa 10%, Saccharomyces cerevisiae 10%,

[0059] Nitrosogenic bacteria 10%, Bacillus megaterium 8%, Aspergillus niger 5%, Paracoccus denitrification 5%.

[0060] Among the above bacteria agents:

[0061] The rhodococcus is Rhodococcus (Rhodococcus rubber) ACCC40324;

[0062] The Bacillus subtilis is Bacillus subtilis ACCC01197;

[0063] The Pseudomonas aeruginosa is Pseudomonas aeruginosa ACCC02722;

[0064] The saccharomyces cerevisiae is Saccharomyces cerevisiae ACCC20203;

[0065] The nitrosogenic bacteria are Nitrosomonas europaea ATCC19718;

[0066] Described Bacillus megatherium is Bacillus megatherium (Bacillus megatherium) ACCC03116;

[0067] Described Aspergillus niger is Aspergillus niger (Aspergillusnige) ACCC30785;

[0068...

Embodiment 3

[0071] In the Linyi Sewage Treatment Plant, using a 50L bucket as the test equipment with stirring, take three 30L water from the explosion pool and add them to the three buckets respectively, adjust the pH to 7.0, and the water temperature is 20°C. Samples are taken to measure COD, ammonia nitrogen, and total nitrogen. Count; COD before processing is 57mg / L, and ammonia nitrogen is 10mg / L, and total nitrogen 30mg / L; Wherein a bucket adds the bacterial agent 20ml that embodiment 1 prepares, another adds the microbial agent 20ml that embodiment 2 prepares, stirs 1 hour, stop stirring after 3 hours and take a sample to measure COD, ammonia nitrogen, total nitrogen number.

[0072]

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Abstract

The invention relates to a biological agent for processing industrial sewage, a preparation method of the biological agent and application. The biological agent is prepared by the following raw materials in percentage by weight; 32% of rhodococcus, 20% of bacillus subtilis, 10% of pseudomonas aeruginosa, 10% of saccharomyces cerevisiae, 10% of nitrosobacteria, 8% of bacillus megatherium, 5% of aspergillus niger and 5% of paracoccus denitrificans. According to the biological agent provided by the invention, the strains are reasonably matched, symbiotic and coordinative and out of antagonism; after being added to a sewage treatment system, the strains are good in effect of degrading macromolecular toxic and harmful substances which are hardly degraded, such as phenols, aromatichydrocarbons, ammonia nitrogen and phosphorus; and the biological agent has unique effect of processing high-concentration organic sewage and high-ammonia nitrogen sewage which are hardly processed by the conventional activated sludge process.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a biological bacterial agent for treating industrial sewage, a preparation method and application thereof. Background technique [0002] With the increasing urban population and the continuous development of industry and agriculture, water pollution accidents occur frequently, seriously endangering the health and even life of people and animals. The discharge of nitrogen and phosphorus in many lakes and reservoirs has caused water eutrophication, which seriously threatens human production, life and ecological balance. Ammonia nitrogen is one of the main factors causing water eutrophication. In order to meet the continuous improvement of the public's requirements for environmental quality, the country has formulated more and more stringent emission standards for nitrogen. Research and development of economical and efficient nitrogen removal treatment technology...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/14C12N1/18C12N1/20C02F3/34C12R1/01C12R1/125C12R1/385C12R1/865C12R1/11C12R1/685
Inventor 何颖霞
Owner 何颖霞
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