Feed additives, premixes and batches containing bacteriocinoids and Pediococcus lactis

A feed additive, Pediococcus lactis technology, used in bacteria, animal feed, animal feed, etc., can solve the problems of not being able to completely get rid of the dependence of antibiotic growth promoters, not fully mobilizing probiotics, and unsatisfactory use, etc. Improve the intestinal microbial environment, prevent diarrhea, and eliminate drug residues

Active Publication Date: 2018-04-03
BEIJING DABEINONG TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, the emergence of microecological preparations, enzyme preparations, Chinese herbal medicine preparations and organic acid additives has played a great role in solving this problem, but the use of many products is not ideal, such as: the use of microbial feed additives can play a role in solving this problem. The effect of supplementing or inoculating bacterial strains, but cannot fully mobilize other probiotics in the intestinal tract; using small peptides and other prebiotic additives alone has a single effect and can activate endogenous probiotics to play a role, but it will lack unique Special strains with nutritional function, immune stimulation and disease resistance
In short, the current full-price compound feed cannot completely get rid of the dependence on antibiotic growth promoters

Method used

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  • Feed additives, premixes and batches containing bacteriocinoids and Pediococcus lactis
  • Feed additives, premixes and batches containing bacteriocinoids and Pediococcus lactis
  • Feed additives, premixes and batches containing bacteriocinoids and Pediococcus lactis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Preparation of Bacillus subtilis CGMCC No.6511 bacteriocins

[0034] 1) Preparation of Bacillus subtilis fermentation broth

[0035] The MNB medium was used for fermentation at a culture temperature of 30° C. for 20 h, the initial pH was 7, the inoculum size was 3%, and the rotation speed of the shaker was 225 rpm.

[0036] The formula of MNB medium is: glucose, 5.0g / L; peptone, 5.0g / L; meat extract, 3.0g / L; yeast extract powder, 1.0g / L; MgSO 4 7H2O, 0.5g / L; MnSO 4 ·H 2 O, 0.005g / L; pH, 7.0.

[0037] 2) Extraction of bacteriocinoids by ammonium sulfate salting-out method

[0038] Prepare the sterile filtrate from the cultivated Bacillus subtilis bacteria liquid, slowly add the ground ammonium sulfate powder into the sterile filtrate, and stir gently to ensure that the ammonium sulfate is completely dissolved, after stirring slowly for 1 hour, place it in a refrigerator at 4°C . Ammonium sulfate gradient treatment was 70%. After overnight, the solutions ...

Embodiment 2

[0048] The preparation of embodiment 2 Pediococcus lactis bacterial powder

[0049] 1) Preparation of Pediococcus lactis CGMCC No.6500 seed solution

[0050] Inoculate the strains preserved on the slant of the test tube into an anaerobic tube containing 20mL of MRS liquid medium, and incubate at a constant temperature for 20 hours at 37°C until the number of viable bacteria reaches 10 7 CFU / mL, inoculate at 1% in a 250mL Erlenmeyer flask filled with 100mL MRS liquid medium, culture anaerobically at 37°C for 20h, until the number of viable bacteria reaches 10 7 CFU / mL (MRS agar medium plate count) or more, as the seed solution for later use.

[0051] 2) Liquid submerged fermentation culture

[0052] The above-mentioned Pediococcus lactis liquid seed solution was added to a 50L fermenter with an inoculation amount of 1%. The culture parameters were: temperature, 37°C; stirring speed, 50rmp; pH value, 6.0±0.5; culture time, 20h. During the fermentation process, 0.5% of the tot...

Embodiment 3

[0060] Preparation of Example 3 Bacteriocins and Pediococcus lactis Mixed Feed Additive

[0061] Prepared by uniformly mixing 50% (wt) of the dry powder of Bacillus bacteriocinoids prepared in Example 1 with 50% (wt) of the powder of Pediococcus lactis prepared in Example 2. Its quality standards are: dry bacteriocinoid powder 102mg / g, live lactic acid bacteria count 12 billion / g, moisture ≤ 10%, arsenic ≤ 0.5mg / kg, lead ≤ 1.0mg / kg.

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Abstract

The invention discloses a new strain HT of pediococcus acidilactici and a feed additive containing bacillus like-bacteriocin and pediococcus acidilactici. The collection number of the new strain of pediococcus acidilactici is CGMCC No.6500. The freeze-dried powder of the like-bacteriocin of bacillus subtilis CGMCC No.6511 is sufficiently mixed with the powder of pediococcus acidilactici HT according to a proportion to obtain the feed additive. The feed additive is a green feed additive which improves the feed conversion rate, enhances the productivity and immunity of animals, prevents diarrhea, and realizes an effect of improving the quality of animal products.

Description

technical field [0001] The invention specifically relates to a feed additive containing bacteriocinoids and Pediococcus lactis, a premix and a batch material, and belongs to the field of microbial feed additives. Background technique [0002] Antibiotics not only solve the contradiction between fast growth and low disease resistance, but also bring huge economic benefits to the aquaculture industry, making antibiotics widely used as feed additives. However, the long-term use of antibiotics in feed has brought many negative effects such as drug residues, drug resistance and environmental pollution, which endanger human health. [0003] As food safety issues are getting more and more attention around the world, the call for reducing or stopping the use of antibiotics in animal husbandry is getting higher and higher. Feed additive raw materials that have medicinal properties and do not pollute the environment have become an inevitable trend. [0004] In recent years, the emer...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/01
Inventor 胡婷贠桂玲李曼韩科厅
Owner BEIJING DABEINONG TECH GRP CO LTD
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