Method for extracting ginsenoside

A technology of ginsenoside and extraction method, which is applied in the field of medicine, can solve the problems of loss of active ingredients, decomposition, and high content of impurities in products, and achieve the effects of being suitable for industrial production, maintaining biological activity, and improving extraction rate

Inactive Publication Date: 2013-03-27
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the traditional extraction method itself has many defects, such as: low extraction yield, loss of active ingredients, decomposition, inactivation, high impurity content in the product, long extraction time, cumbersome process, etc.; although ultrasoni

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  • Method for extracting ginsenoside

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Experimental program
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Effect test

Embodiment 1

[0023] a. Crush the ginseng raw materials, pass through a 40-mesh sieve, and then put them into the extraction tank;

[0024] b. Add laccase into the extraction tank, the weight-volume ratio of ginseng powder to the crude enzyme extract is 1kg:10L, enzymatically hydrolyze at 60°C for 2h, filter with 200 mesh filter cloth;

[0025] c. Add methanol to the filter residue, the weight-volume ratio of the filter residue to methanol is 1kg:100L, after soaking for 0.5h, supplemented with microwave action for 3min, microwave power is 300W, continue to extract for 1h, filter, the filtrate is concentrated under reduced pressure, and dried The crude ginsenoside is obtained.

Embodiment 2

[0027] a. Crush the ginseng raw materials, pass through a 40-mesh sieve, and then put them into the extraction tank;

[0028] b. Add lignin peroxidase to the extraction tank, the weight-volume ratio of ginseng powder to the crude enzyme extract is 1kg:20L, enzymatically hydrolyze at 60°C for 1 hour, and filter with 200 mesh filter cloth;

[0029] c. Add ethanol to the filter residue, the weight-volume ratio of the filter residue to ethanol is 1kg:150L, ​​after soaking for 0.5h, supplemented with microwave action for 3min, microwave power is 300W, continue to extract for 1.5h, filter, concentrate the filtrate under reduced pressure, and dry Then the crude ginsenoside was obtained.

Embodiment 3

[0031] a. Crush the ginseng raw materials, pass through a 40-mesh sieve, and then put them into the extraction tank;

[0032] b. Add manganese peroxidase into the extraction tank, the weight-volume ratio of ginseng powder to the crude enzyme extract is 1kg:50L, enzymatically hydrolyze at 0°C for 2h, filter with 200 mesh filter cloth;

[0033] c. Add methanol to the filter residue, the weight-volume ratio of the filter residue to methanol is 1kg:100L, after soaking for 0.5h, assisted by microwave action for 5min, the microwave power is 300W, continue to extract for 0.5h, filter, concentrate the filtrate under reduced pressure, and dry Then the crude ginsenoside was obtained.

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Abstract

The invention belongs to the field of medical technology, and particularly relates to a method for extracting ginsenoside. The method for extracting ginsenoside comprises the following steps of: smashing and sieving raw ginseng, adding 90-120U/mL of crude enzyme extract, conducting enzymolysis for 0.5-2hours at 50-70 DEG C, filtering, adding an organic solvent into residual slag, conducting microwave extraction and then conducting digestion, filtering, decompressing and concentrating the filtrate, and drying to obtain crude ginsenoside. The ginsenoside is extracted from the ginseng by adopting a method for combining an enzymic method and organic solvent extraction, the enzyme added in the method can effectively degrade lignin, prompt the release of active constituents of plants and greatly improve the extraction rate of the active constituents; and according to the extraction method, microwave action is assisted in the organic solvent extraction process, the extraction of effective constituents can be further increased, the bioactivity can be better maintained, and the method is more suitable for industrial production.

Description

Technical field [0001] The invention belongs to the technical field of medicine, and relates to a method for extracting traditional Chinese medicine, in particular to a method for extracting ginsenosides from traditional Chinese medicine ginseng. Background technique [0002] Ginseng is the dried root of Panax ginseng C. A. Mey. The main active ingredient of ginseng is ginsenosides. Pharmacological studies have shown that the main pharmacological effects of ginseng are improving immunity, anti-fatigue, anti-stress, improving microcirculation, improving tissue anti-hypoxia ability, inhibiting platelet aggregation, Ca 2+ Antagonism, influence on prostaglandin (PG5) metabolism, anti-tumor, anti-aging, anti-radiation, etc. [0003] At present, the domestic extraction of ginsenosides mainly adopts heating reflux method, water extraction and alcohol precipitation method, microwave extraction method, and ultrasonic extraction method. However, the traditional extraction method itself has...

Claims

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Application Information

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IPC IPC(8): A61K36/258A61P37/04A61P39/00A61P7/02A61P35/00A61P17/16A61K125/00
Inventor 张成如
Owner SHANDONG UNIV
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