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Weak bitter corn oligopeptide with high content of alanine and leucine, and preparation method thereof

A corn oligopeptide and leucine technology, applied in the direction of fermentation, can solve the problems of difficult industrialization, difficult to accept, and high manufacturing cost, and achieve the effects of easy industrialization, improving product yield, and reducing hydrophobicity

Active Publication Date: 2013-03-27
广州合诚实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The outstanding problems in the preparation of corn oligopeptides include four aspects: (1) The product has low purity, that is, the protein content of corn oligopeptides is low, mainly because the purification of corn gluten powder in the early stage was not done properly, which affected the quality of the product; ( 2) The preparation process is cumbersome, without scientific optimization, high cost, and difficult to implement industrially; (3) The taste of corn active peptides is bitter and even irritating, which is difficult to be accepted by the public, and the promotion and application are limited; (4) Corn oligopeptides have A variety of physiological effects, but no research and development for a specific effect, the product effect is not outstanding
For example, patent CN101531700A discloses that corn yellow powder (about 60% protein content) is used as raw material, treated with alkaline water to remove impurities such as starch and oil to obtain high-purity corn protein slag, and then the slag is pretreated, enzymatically hydrolyzed, and centrifugally filtered , concentrating and drying to prepare corn oligopeptide powder with a protein content of more than 85% and a molecular weight distribution below 2000Da. In this invention, the amount of alkaline protease is 5% to 8%, the amount of neutral protease is 5% to 8%, and the total enzyme The dosage reaches 10%~16%, the consumption of water, alkali and acid is large, the product yield (the ratio of corn peptide to corn gluten powder mass) is 22.3%~24%, and the manufacturing cost of the product is very high; in addition, there is no decolorization and decolorization in the patent Odor technology, no description of product flavor, and no specific design for a certain effect of the product; patent CN102028093A discloses that corn yellow powder is used as raw material, and alkaline protease Alcalase and composite protease Protamex are used for segmental enzymolysis, and the enzymatic hydrolysis products are centrifuged. The supernatant was ultrafiltered by ultrafiltration membranes with molecular weight cut-offs of 100kDa and 10-6kDa respectively, and then the filtrate was concentrated and spray-dried to obtain corn hangover peptide powder. In this product, peptides with a molecular weight of less than 1200Da accounted for 73.19%, and alanine The content of corn peptide and leucine is greater than 6.43% and 12.42% respectively. The corn peptide purity of the product is low and the flavor is not described. In addition, because the invention only removes macromolecular substances through rough separation and ultrafiltration, the free amino acid content, The ash content does not provide data, and the content of alanine and leucine that actually exist in the form of oligopeptides is not clear; patent CN102172293A discloses a preparation method of corn active small peptide hangover agent, which uses alkaline water to treat corn gluten powder for purification, and then Add 4-5% alkaline protease Alcalase, flavor protease Flavourzyme and complex protease Protamex 1-3% for segmental hydrolysis, and the supernatant after centrifugation of the enzymatic hydrolysis product is separated by a secondary ultrafiltration membrane to obtain small peptides with a main molecular weight of 200-1000Da. This method uses a large amount of enzyme, reaching 5% to 8%.

Method used

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  • Weak bitter corn oligopeptide with high content of alanine and leucine, and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0040] (1) Dispersion and hydration of corn gluten powder: crush corn gluten powder (protein content ≥ 60%, purchased from Shanxi Wang Group) to 80 mesh, and prepare corn gluten powder with deionized water to a mass percentage of 10% corn gluten solution, disperse and hydrate the corn gluten powder for 20 minutes with a mixer at a speed of 2000 rpm;

[0041] (2) Gelatinization, degreasing, and starch removal: use a molar concentration of 3mol.L -1 Sodium hydroxide solution to adjust the pH of the corn gluten powder solution to 6.0, heat it to 82°C, and keep it for 40 minutes, so that the molecular structure of the corn gluten is fully expanded, and the starch in it is fully gelatinized; add 0.5 % α-high temperature amylase (SUKAMy-Hi LQ20, SUKAMy-Hi LQ20, Sukehan Bioengineering Co., Ltd.), hydrolyzed at 36rpm, 82°C for 45min, after the end of the enzymolysis, the enzymatic solution was kept at 95°C for 15min, and the α-blunt Inactivate the activity of high-temperature amylase...

Embodiment 2

[0046] (1) Dispersion and hydration of corn gluten powder: crush corn gluten powder (protein content ≥ 60%, purchased from Shanxi Wang Group) to 80 mesh, and prepare corn gluten powder with deionized water to a mass percentage of 20% corn gluten solution, disperse and hydrate the corn gluten powder with a mixer at a speed of 3000 rpm for 10 minutes;

[0047] (2) Gelatinization, degreasing, and starch removal: use a molar concentration of 5mol.L -1 Adjust the pH of the corn gluten powder solution to 7.0 with a sodium hydroxide solution, heat it to 80°C, and keep it for 60 minutes to fully expand the molecular structure of the corn gluten and fully gelatinize the starch in it; add 1 % α-high temperature amylase (SUKAMy-Hi LQ20, SUKAMy-Hi LQ20, Sukehan Bioengineering Co., Ltd.), hydrolyzed at 36rpm, 80°C for 60min, after the end of the enzymolysis, the enzymatic solution was kept at 95°C for 20min, and the α-blunt Inactivate the activity of high-temperature amylase, then cool do...

Embodiment 3

[0052] (1) Dispersion and hydration of corn gluten powder: crush corn gluten powder (protein content ≥ 60%, purchased from Shanxi Wang Group) to 80 mesh, and prepare corn gluten powder with deionized water to a mass percentage of 5% corn gluten solution, disperse and hydrate the corn gluten powder with a mixer at a speed of 2500 rpm for 15 minutes;

[0053] (2) Gelatinization, degreasing, and starch removal: use a molar concentration of 1mol.L -1 Sodium hydroxide solution to adjust the pH of the corn gluten powder solution to 5.0, heat it to 85°C, and keep it for 20 minutes, so that the molecular structure of the corn gluten is fully expanded, and the starch in it is fully gelatinized; add 0.1 % α-high temperature amylase (SUKAMy-Hi LQ20, SUKAMy-Hi LQ20, Sukehan Bioengineering Co., Ltd.), hydrolyzed at 36rpm and 85°C for 30min, after the end of the enzymolysis, the enzymatic solution was kept at 100°C for 10min, and the α-blunt Inactivate the activity of high-temperature amyl...

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Abstract

The invention belongs to the field of agricultural and sideline product processing and utilization technology with high added value, and discloses a weak bitter corn oligopeptide with high content of alanine and leucine, and a preparation method of the weak bitter corn oligopeptide. The method comprises the steps of: (1) dispersing and hydrating corn gluten meal; (2) carrying out gelatinization and alpha-high temperature amylase enzymolysis, degreasing and removing starch; (3) carrying out enzymolysis; (4) carrying out enzyme deactivation and decoloration; and (5) carrying out rough filtration, ultrafiltration and nanofiltration, and drying to obtain the weak bitter corn oligopeptide. The weak bitter corn oligopeptide contains 85.42-87.63% by mass of protein percent, 92.44-94.21% by mass of peptide with the relative molecular weight being less than 1000Da, 6.75-6.94% by mass of alanine, 14.12-14.66% by mass of leucine and 1.12-1.36% by mass of free amino acid. The weak bitter corn oligopeptide with high content of alanine and leucine can be widely used as raw material of health-care food and common food, and especially can be used in products used for sobering up and protecting the liver.

Description

technical field [0001] The invention belongs to the technical field of high value-added processing and utilization of agricultural and sideline products, and in particular relates to a weakly bitter corn oligopeptide with high content of alanine and leucine and a preparation method thereof. Background technique [0002] Corn is one of the three major food crops in the world, and it is also a traditional crop in my country. In 2009, my country's corn production reached 164 million tons, accounting for about 20% of grain production, ranking second in the world. One of the main uses of corn is to produce corn starch. The main by-product in the process of producing corn starch by wet milling is corn gluten meal (Corn Gluten Meal, CGM), also known as yellow powder. Corn gluten meal generally contains about 60% protein, mainly The ingredients are zein (68%), gluten (22%) and globulin (1.2%). Corn protein contains a high proportion of hydrophobic amino acids such as leucine, isol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 周雪松申世强曾建新蒋文真
Owner 广州合诚实业有限公司
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