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Annulus fibrosus and nucleus pulposus integrated composite biphasic scaffold and construction method thereof

A composite biphasic, construction method technology, applied in prosthesis, medical science and other directions, to avoid immune rejection, a wide range of material sources, good biocompatibility and degradability

Active Publication Date: 2013-04-03
TIANJIN HOSPITAL
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  • Abstract
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  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a bioengineering annulus fibrosus and nucleus pulposus that is suitable for the growth requirements of annulus fibrosus cells and nucleus pulposus cells to address the shortcomings of only a single tissue engineering annulus fibrosus scaffold or tissue engineering nucleus pulposus scaffold in the prior art. Integrated composite biphasic scaffold and preparation method thereof

Method used

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  • Annulus fibrosus and nucleus pulposus integrated composite biphasic scaffold and construction method thereof
  • Annulus fibrosus and nucleus pulposus integrated composite biphasic scaffold and construction method thereof
  • Annulus fibrosus and nucleus pulposus integrated composite biphasic scaffold and construction method thereof

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Embodiment Construction

[0047] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0048] 1. Preparation of acellular decalcified bone matrix gelatin rings

[0049] Fresh porcine femoral head cancellous bone was used as raw material, trimmed to make a hollow bone ring with an outer diameter of 10 mm, an inner diameter of 5 mm, and a thickness of 3 mm, and decellularized and decalcified at room temperature. Add 1500ml of chloroform-methanol mixture to 50g of cancellous bone, soak for 24h; ② soak in 0.6mol / L HCl for 48h; ③2 mmol / L CaCl 2Treatment for 1 hour; ④ Soak in 0.5 mmol / L ethylenediaminetetraacetic acid (EDTA) for 1 hour; ⑤ Treat with 8 mmol / L lithium chloride (LiCl) for 1 hour; ⑥ Soak in 5% Triton-100 for 24 hours; ⑦ Deionization Rinse with water repeatedly and set aside.

[0050] 2. Preparation of decellularized nucleus pulposus matrix microfilament suspension

[0051] Collect the nucleus pulposus tissue from the pig tail,...

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Abstract

The invention discloses an annulus fibrosus and nucleus pulposus integrated composite biphasic scaffold and a construction method thereof, and belongs to the technology of biological tissue engineering. Porcine cancellous bone is used for preparing the outer layer which is a cell-free deminerized bone matrix gelatin ring, pigtail intervertebral disc nucleus gelatinosus tissue is used for preparing the core which is a cell-free nucleus pulposus matrix; and the two materials are frozen and lyophilized, sequentially subjected to ultraviolet irradiation crosslink and chemical reagent EDAC (carbodiimide) and NHS (n-hydroxysuccinimide) crosslink, and compounded. The annulus fibrosus and nucleus pulposus integrated composite biphasic scaffold with the design is good in biocompatibility to avoid immunological rejection reactions, has a composition structure and mechanical performance similar to a human intervertebral disc extracellular matrix, is wide in material source, low in cost and simple in preparation process, can be used for constructing tissue engineering intervertebral disc and repairing intervertebral disc degeneration, and has an excellent clinical application prospect.

Description

technical field [0001] The invention relates to biological tissue engineering technology, in particular to a composite biphasic scaffold integrated with annulus fibrosus and nucleus pulposus and its construction method Background technique [0002] Biological tissue engineering methods mainly include three elements: scaffold materials, seed cells, and signaling factors. Among them, scaffold materials are the key link in the construction of tissue engineering. Ideal tissue engineered intervertebral disc scaffold materials should not only have good biocompatibility, good pore structure, and appropriate degradation properties, but also be similar to human intervertebral disc extracellular matrix in terms of composition, shape, structure, and mechanical properties. [0003] The structure of the intervertebral disc is complex and has a high degree of heterogeneity. It contains two developmentally and morphologically different regions, the nucleus pulposus and the annulus fibrosus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/22
Inventor 杨强徐宝山许海委马信龙李秀兰夏群张春秋
Owner TIANJIN HOSPITAL
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