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Sulfur ion measurement method based on gold nanoparticles as simulated peroxidase

A technology of peroxidase and sulfide ion, applied in analytical chemistry and nano fields, can solve problems such as limitations, and achieve the effects of low detection cost, high detection sensitivity, and simple and fast preparation process

Inactive Publication Date: 2013-04-17
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the complex modification process of gold nanoparticles in these methods limits their application to a certain extent.

Method used

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  • Sulfur ion measurement method based on gold nanoparticles as simulated peroxidase
  • Sulfur ion measurement method based on gold nanoparticles as simulated peroxidase
  • Sulfur ion measurement method based on gold nanoparticles as simulated peroxidase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Dilute 500 μL of 0.1 g / L chloroauric acid aqueous solution with 39.5 ml of water, add 0.8 ml of 0.1 g / L sodium borohydride aqueous solution under vigorous stirring (the addition time is controlled within 5 minutes), the color of the reaction solution From light yellow to wine red, continue to stir quickly for 1 hour in the dark. The average particle size of the obtained nano-gold is 8.1±1.1 nm and stored at 4°C. The nano-gold can be kept for at least two months and is relatively stable. All the glassware used in the above process is soaked in aqua regia, thoroughly washed with double distilled water, and dried.

Embodiment 2

[0035] Take 0.05 ml of the nano gold solution prepared in Example 1 and add 0.075 ml of hydrogen peroxide with a concentration of 8 mol / L and 0.05 ml of 3,3',5,5'-tetramethylbenzidine with a concentration of 16 mmol / L. Hydrochloride, 0.825 ml phosphate buffer solution (pH=4, 20 mmol / L), after mixing, incubate at 45 ℃ for 10 minutes, the mixed solution turns blue, and the maximum absorption wavelength is 652 nm (see figure 1 ).

Embodiment 3

[0037] Take 0.15 ml of the sulfide ion sample solution with a concentration of 20 μmol / L and add 0.05 ml of the nano-gold solution prepared in Example 1. After mixing, place it at room temperature for 1 minute, and add 0.075 ml of the solution with a concentration of 8 mol / L to the mixed solution. Hydrogen oxide, 0.05 ml 3,3',5,5'-tetramethylbenzidine hydrochloride with a concentration of 16 mmol / L, 0.675 ml phosphate buffer solution (pH=4, 20 mmol / L), after mixing Incubate at 45 ℃ for 10 minutes, the mixed solution is still colorless, almost no absorption at the wavelength of 652 nm (see figure 2 ).

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Abstract

The invention discloses a sulfur ion measurement method based on gold nanoparticles as simulated peroxidase. The gold nanoparticles act with sulfur ions to simulate the change of activity of peroxidase, and 3,3',5,5'-tetramethylbenzidine hydrochloride is oxidized by hydrogen peroxide in the presence of the gold nanoparticles as catalyst, and the color is developed to indicate the change of solution color and the change of ultraviolet absorption spectral characteristics. The detection limit of visual observation is 2 Mumol / L. The linear range of absorbance detection is 0.5-10 Mumol / L, and the detection limit is 0.08 Mumol / L. After the simple pretreatment of a water sample, the content of sulfur ions in the water sample is measured according to the method disclosed by the invention.

Description

technical field [0001] The invention relates to a method for quickly measuring the content of sulfide ions using nano gold as a simulated peroxidase, belonging to the fields of analytical chemistry and nanotechnology. Background technique [0002] Sulfide is a common chemical raw material and chemical pollutant in papermaking, petrochemical, leather and other industries, which is harmful to aquatic ecosystems and human health. Under acidic conditions, sulfide is transformed into hydrogen sulfide, which can harm cytochrome and oxidase, cause hypoxia in cells and tissues, and even endanger life. At the same time, hydrogen sulfide also corrodes metal equipment and pipelines, and can be oxidized into sulfuric acid by microorganisms, which intensifies corrosion. Therefore, sulfide is one of the important indicators of water pollution, and it is necessary to establish a reliable and highly sensitive method that can provide on-site real-time detection of sulfide in water. At pres...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N21/78
Inventor 陈伟邓豪华洪磊刘爱林林新华李光文
Owner FUJIAN MEDICAL UNIV
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