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Autologous biomedical fibrin glue preparation for plugging digestive tract fistula as well as preparation method and application of autologous biomedical fibrin glue preparation

An autologous biology and digestive tract technology, applied in biochemical equipment and methods, animal/human protein, digestive system, etc., can solve the problems of lack of platelets, limited ability of tissue healing, etc., to prolong the existence time in the body, and the preparation method is simple Feasible, more biocompatible results

Active Publication Date: 2013-05-22
NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current commercial gels are prepared from platelet-poor plasma, which lacks platelets and thus has limited ability to promote tissue healing

Method used

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  • Autologous biomedical fibrin glue preparation for plugging digestive tract fistula as well as preparation method and application of autologous biomedical fibrin glue preparation
  • Autologous biomedical fibrin glue preparation for plugging digestive tract fistula as well as preparation method and application of autologous biomedical fibrin glue preparation
  • Autologous biomedical fibrin glue preparation for plugging digestive tract fistula as well as preparation method and application of autologous biomedical fibrin glue preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Preparation of high-concentration platelet plasma: add 28g of citrate glucosylphosphate adenine anticoagulant (CPD) to 200ml of whole blood, centrifuge at 1000g for 6min at 25°C, separate the uppermost layer of plasma, and repeat the plasma under the same centrifugation conditions After one centrifugation, the uppermost layer of plasma is separated as high-concentration platelet plasma (PRP);

[0040] (2) Preparation of fibrinogen: Freeze the high-concentration platelet plasma (PRP) prepared in step (1) at -80°C for 1-6 hours, then thaw at 4°C for 30 minutes or until the plasma is completely melted, and then 4000rpm / min Centrifuge for 5 minutes, suck out the supernatant, and keep the sediment at the bottom to obtain concentrated fibrinogen;

[0041] (3) Preparation of thrombin: Take 10g of PRP as an example, transfer 10g of PRP prepared in step (1) to a 150ml blood bag, add 90ml of citric acid (2.84mM) to dilute, centrifuge at 3000g for 5min at 4°C, discard the supe...

Embodiment 2

[0053] The platelet-rich biological protein glue prepared according to the method of Example 1 has been clinically applied in the intestinal fistula area of ​​Nanjing General Hospital of Nanjing Military Region. The patient's inclusion criteria are postoperative or traumatic intestinal fistula; 200ml / d); sinus tract length > 2cm; general condition is good; age > 18 years old. Exclusion criteria include: obstruction of the intestinal tract at the distal end of the fistula, complete or rupture of more than 1 / 3 of the intestinal tract; severe liver cirrhosis, diabetes and other metabolic disorders; tumors, specific infections or intestinal inflammatory diseases; intestinal fistula sinus The tract is complicated, with two or more bifurcations; there is a pus cavity around; the intestinal fistula mucosa is completely everted and adheres to the surrounding skin to form a labial fistula, with no hope of self-healing at all; blood donation is contraindicated (hypertension, blood donati...

Embodiment 3

[0068] As an excellent biological scaffold, FG can provide a suitable environment for the growth of organelles, and can overcome the shortcomings of artificial materials such as foreign body reactions, slow degradation, and toxicity. Bone marrow or adipose-derived mesenchymal stem cells (MSCs) in adult stem cells can be obtained through non-invasive operations, and also have the potential for multi-directional differentiation, which can be differentiated into liver round cells, liver cells, cholangiocytes, skeletal muscle cells, nerve cells, etc. Yuan and even renal tubular epithelial cells. Compared with bone marrow stem cells, adipose-derived stem cells (ADSCs) are abundant and easy to extract, and can be differentiated into chondrocytes, adipocytes, osteoblasts, etc., and have been widely used clinically. An autologous biological protein glue containing human adipose stem cells, prepared by the following method:

[0069] (1) Preparation of high-concentration platelet plasm...

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Abstract

The invention discloses autologous biomedical fibrin glue preparation for plugging digestive tract fistula as well as a preparation method and application of the autologous biomedical fibrin glue preparation. The autologous biomedical fibrin glue preparation comprises the following components: FG (Fibrinogen) component I-Fibrinogen, 1mg / ml tranexamic acid solution and FG component II-thrombin, wherein the fibrinogen and the thrombin are prepared from high-concentration platelet-rich plasma. The invention further discloses the application of the autologous biomedical fibrin glue preparation for plugging digestive tract fistula in preparing medicaments or medical apparatus and instruments for treating full-digestive tract fistula. The invention has the following advantages: the preparation method is simple and easy to apply, the preparation cost is low, and the needs of the mass are satisfied; the biomedical fibrin glue is taken from a patient, so that the blood transfusion risks of heterologous blood products or xenogenous blood products are avoided; and besides, the biocompatibility is better, and no report about the adverse reaction exists until now according to the clinical tests.

Description

technical field [0001] The invention belongs to the field of peptide-containing medical supplies, and relates to an autologous biological protein glue reagent for blocking digestive tract fistula, a preparation method and application thereof. Background technique [0002] Although modern medicine has made great progress in surgical techniques and preoperative preparation, gastrointestinal anastomotic leakage still occurs from time to time. Anastomotic leakage not only increases mortality, but also has adverse effects on long-term survival. Therefore, the treatment of fistula is still a difficult problem in today's medicine. Traditional surgical treatment methods not only bring heavy economic burdens to patients and their families, but also have high surgical risks. As a national intestinal fistula treatment center, seeking the best intestinal fistula treatment plan and promoting the rapid healing of intestinal fistula has always been the direction of our research. For pati...

Claims

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Application Information

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IPC IPC(8): A61K38/48A61L31/04C07K14/75C12N9/74A61P1/00A61K31/195A61K33/38A61K35/12A61K38/36A61K35/28
Inventor 任建安吴秀文栾建凤姚根宏顾国胜周波陈军洪之武袁玉杰陈钰黎介寿
Owner NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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