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Preparation method of three-dimensional cell culture support and culture device of three-dimensional cells

A three-dimensional cell and culture scaffold technology, which is applied in the preparation of cell culture scaffolds, the preparation of three-dimensional cell culture scaffolds, and the field of cell culture devices to achieve the effects of good growth, maintenance and proliferation.

Inactive Publication Date: 2013-06-05
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Therefore, so far, there is still no cell scaffold that can fully meet the needs of adhesion, infiltration, continuous differentiation and long-term growth of various cells at the same time.

Method used

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  • Preparation method of three-dimensional cell culture support and culture device of three-dimensional cells
  • Preparation method of three-dimensional cell culture support and culture device of three-dimensional cells
  • Preparation method of three-dimensional cell culture support and culture device of three-dimensional cells

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preparation example Construction

[0043] In this embodiment, the preparation method of the three-dimensional cell culture scaffold includes the following steps:

[0044] a. Weigh 1~10g of polymer and dissolve it in 10~100ml of organic solvent to form a polymer solution. The polymer includes polyvinylchloride (PVC), etc., and the organic solvent includes tetrahydrofuran, etc.

[0045]b. Weigh 0~500mg of plasticizer, add it into the above polymer solution, and mix well. Plasticizers include dioctyl phthalate, dioctyl sebacate, and the like.

[0046] c. Weigh 0~2g of hydroxyapatite glue nanoparticles; 0~2g of silver nanoparticles; 0~2g of zinc oxide nanoparticles; 0~2g of chitosan nanoparticles, join in the solution processed through step b, mix Evenly, the spinning dope is obtained. Wherein the particle size range of the above-mentioned nanoparticles is: 10-500nm.

[0047] d. Inject the spinning stock solution into the electrospinning equipment, perform electrospinning treatment, and obtain spinning on the re...

Embodiment 1

[0056] Example 1: Preparation of three-dimensional cell culture scaffold by electrospinning method

[0057] In this specific embodiment, the polymer is: polyvinylchloride (PVC); the organic solvent is: tetrahydrofuran; and the plasticizer is: dioctyl phthalate.

[0058] Take 5g of polyvinyl chloride powder and dissolve it in 65ml of tetrahydrofuran. According to the experiment, an appropriate amount of plasticizer dioctyl phthalate 275mg can be added. 1.2g of 500nm hydroxyapatite glue nanoparticles, 0.9g of silver nanoparticles, 1.5g of zinc oxide nanoparticles, 0.8g of chitosan nanoparticles, and mix them uniformly. Then, under the conditions of voltage 45kV, receiving distance 17cm, sampling speed 1.8ml per hour, and receiving screen made of aluminum foil (roller can also be used to collect and spin), an orderly spinning is produced, and then the spinning is dried, that is Three-dimensional cell scaffolds for cell culture.

[0059] Such as image 3 Shown is a schematic di...

Embodiment 2

[0060] Example 2: Preparation of three-dimensional cell culture scaffold by using porogen pore-making method

[0061] In this specific embodiment, the first matrix material is: L-polylactic acid; the second matrix material is: addition polycaprolactone; the organic solvent is: N,N-dimethylformamide; the acidic solution is hydrofluoric acid.

[0062] Weigh 38g of L-polylactic acid, add 42g of polycaprolactone and 7.5g of N,N-dimethylformamide. Add L-polylactic acid and polycaprolactone into N, N-dimethylformamide, and mix well. Then, add 0.8g of hydroxyapatite glue nanoparticles of 10-500nm, 1.1g of zinc oxide nanoparticles, 0.5g of silver nanoparticles, and 1.3g of chitosan nanoparticles and mix well, and add the compound with a diameter of 10-500nm. The 50-micron silica particles are mixed into various shapes, and after drying, they are soaked in hydrofluoric acid to dissolve the silica particles to obtain a round-hole cell culture scaffold.

[0063] Such as Figure 4 Show...

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Abstract

The invention provides a preparation method of a three-dimensional cell culture support. The preparation method includes the following steps: (a) weighting 1-10g of polymer and dissolving the polymer in an organic solution of 10-100ml, and making a polymer solution; (b) weighting 0-500mg of a plasticizer, adding the plasticizer into the polymer solution, and mixing the solution uniformly; (c) weighting 0-2g of hydroxyapatite gel nanoparticles, 0-2g of silver nanoparticles, 0-2g of zinc oxide nanoparticles and 0-2g of chitosan nanoparticles, adding the nanopaticles into the solution treated in the step (b), mixing the solution uniformly and obtaining spinning original liquid; (d) injecting the spinning original liquid into an electrostatic spinning device and obtaining spun yarns on a receiving device of the electrostatic spinning device; (e) carrying out drying treatment on the obtained spun yarns to obtain the three-dimensional cell culture support. The invention further provides a culture device of three-dimensional cells, wherein the culture device is obtained on the basis of the preparation method of the three-dimensional cell culture support. The culture device can provide non-toxic growing environment which has biocompatibility for cultured cells, and meanwhile is suitable for culture of various kinds of the cells.

Description

technical field [0001] The invention relates to a method for preparing a cell culture scaffold, in particular to a method for preparing a three-dimensional cell culture scaffold applicable to various cell cultures, and belongs to the technical field of cell culture. [0002] The invention also relates to a cell culture device, in particular to a three-dimensional cell culture device applicable to various cell cultures, belonging to the technical field of cell culture. Background technique [0003] The technical means of studying and observing the morphological structure and life activities of cells have been used in various scientific researches such as cytology, genetics, immunology, experimental medicine and oncology. It has become a necessary pre-screening and pre-experiment method for life research. Two-dimensional cell culture and model building have made great contributions to our in-depth study of cell behavior, infection and disease pathogenesis. Genetic engineering...

Claims

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Application Information

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IPC IPC(8): C12M3/00D01D1/02D01D5/00D01F6/48D01F1/10C08J9/26C08L67/04C08L5/08C08K3/32C08K3/22C08K3/08
Inventor 唐祖明周雪锋梅茜顾宁
Owner SOUTHEAST UNIV
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