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Sea cucumber extract containing alkaloids active ingredients as well as extracting method and application thereof

A technology of active ingredients and alkaloids, applied in the field of sea cucumber extracts, can solve the problems of wasting resources, unable to quantify effective doses, etc., and achieve the effects of reducing organic solubility, improving the immune function of the body, and retaining active ingredients

Active Publication Date: 2013-09-04
刘平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there are problems in various extraction processes and preparations of sea cucumber preparations at present, which may cause waste of resources, and the effective dosage of various preparations cannot be quantified
These problems bring certain blindness to the application of sea cucumber in medicine and food

Method used

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  • Sea cucumber extract containing alkaloids active ingredients as well as extracting method and application thereof
  • Sea cucumber extract containing alkaloids active ingredients as well as extracting method and application thereof
  • Sea cucumber extract containing alkaloids active ingredients as well as extracting method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1 Preparation of sea cucumber extract containing alkaloid active ingredients

[0065] Take 30 grams of sea cucumber powder, add 2400 milliliters of distilled water, stir at 56 ° C for 4 hours, and refrigerate overnight. The leachate was centrifuged at 6000 rpm for 40 minutes, and the sediment was discarded. The leachate supernatant was filtered through a 0.8 μ filter membrane. The filtrate is ultrafiltered through a 10KD ultrafiltration membrane to obtain an ultrafiltered external solution. The ultrafiltration external liquid is filtered through the nanofiltration membrane to obtain the concentrated internal liquid through nanofiltration. The concentrated inner solution was concentrated by rotary evaporation at 56°C to obtain a nanofiltration concentrate. The nanofiltration concentrate was passed through a D101 macroporous resin column, the eluent was distilled water, the eluted peak was discarded, and 30% ethanol was changed to continue elution, and the e...

Embodiment 2

[0067] Example 2 Effects of sea cucumber extract and various monomer compounds on the proliferation of bone marrow mesenchymal stem cells (MSCs) in model mice treated with 5-fluorouracil

[0068] Experimental method: Take 10 mice, inject 5-fluorouracil (5-FU) 150mg / kg body weight into the tail vein, take femoral bone marrow cells after 48 hours, and culture them to obtain mouse bone marrow mesenchymal stem cells (MSCs), culture method reference .

[0069] Cells were seeded in 96-well culture plates at a density of 1.0×10 4 Each cell / well was 100 μl, and the cells were replaced with serum-free medium 24 hours after seeding. The same volume of culture medium was added to the control group, macrophage-granulocyte colony-stimulating factor (GM-CSF) was added to the positive drug, and sea cucumber extract (HS), H2a, H2b, H2c, H2d and H2e and corresponding samples were added to the drug-dosed group. Compound standards of Inosine, Adenosine, Guanosine, Uridine and Thymidine, the fi...

Embodiment 3

[0079]Example 3 Protective effect of sea cucumber extract and various monomer compounds on neonatal rat cardiomyocyte hypoxia / reoxygenation injury

[0080] Experimental method: Suckling rat cardiomyocyte culture: aseptically remove the 3-day-old suckling rat heart, remove the pericardium, take the apex myocardial tissue and cut it into 1mm3 small pieces, wash it twice with D-Hank's solution, add 0.125% trypsin , digested at 37°C for 20min. Prepare the cell suspension by pipetting the tissue pieces, centrifuge for 10 min and discard the supernatant. Add D-Hank's solution, centrifuge for 10min, discard the supernatant and wash twice. The cell suspension was added to DMEM culture medium containing 15% fetal bovine serum, and placed in a 25ml culture bottle. Incubate at 37°C for 60 minutes. Take the cells in the culture medium and discard the adherent cells. The cardiomyocytes purified by differential adherence were prepared into a cell suspension again, inoculated into a 96-w...

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Abstract

The invention discloses a sea cucumber extract containing alkaloids active ingredients as well as an extracting method and an application thereof. The sea cucumber extract is extracted by comprising the following steps of: weighing sea cucumber powder, and adding distilled water of 56 DEG C into the sea cucumber powder for stirring; centrifuging a lixivium and discarding sediments; filtering a supernate with a filtering membrane, and ultra-filtering a filtrate through an ultrafiltration membrane; concentrating an external ultrafiltrate through a nanofiltration membrane to obtain an internal nanofiltration concentrated solution; carrying out rotary evaporation and concentrating the concentrated solution again; enabling the concentrated solution to pass through a macroporous resin column; eluting the concentrated solution with distilled water and discarding an elution peak; eluting the concentrated solution with 30% ethanol and adopting the elution peak; and after evaporation and concentration, enabling the elution peak to pass through DA25 anion chromatography, adopting an active ingredient peak and freeze-drying the active ingredient peaks to obtain freeze-dried powder of five alkaloids active ingredients including H1a, H2b, H2c, H2d and H2e. Experiments prove that the sea cucumber extract and an alkaloid compound containing the same have potential uses for treating anemia, immunocom promise, wound and fatigue recovery and chemoradiotherapy-induced immune repairing.

Description

technical field [0001] The present invention relates to extracts containing alkaloid active ingredients derived from animals, in particular to sea cucumber extracts containing bioactive substances that are stereoisomers with inosine, adenosine, guanosine, uridine and deoxythymidine and Its extraction method and application. At the same time, it also involves pharmaceutical preparations mainly containing active ingredients derived from animals. Background technique [0002] Sea cucumber class (Holothuroidea or Holothurioidea) is the most economically significant class in the Echinodermata (Echinodermata). Among them, there are 3 families (Synallatidae, Holothuriidae, Stichopodidae) in the order of Chiroptera, and 3 genera (Apostichopus, Stichopus, and Thelenota) . The genus Apostichopus japonicus is widely distributed in Dalian, Lushun, Haiyang Island and Shandong coastal areas in Liaoning. my country has used it as a gourmet food since ancient times. Traditional Chinese...

Claims

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Application Information

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IPC IPC(8): A61K31/708A61P7/06A61P17/02A61P37/04A61P1/02A61P1/00A61P11/00A61P27/02A23L1/30C07H19/167C07H19/067C07H19/073C07H1/08A61K31/7076A61K31/7072
Inventor 刘平葛迎春
Owner 刘平
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