Analysis and detection method of surface enhanced Raman of benzo (a) pyrene

A surface-enhanced Raman and detection method technology, applied in Raman scattering, material excitation analysis, etc., can solve the problems of weakened SERS enhancement effect, easy oxidation, unstable silver film, etc., and achieve simple production, uniform particle size, Prepare simple effects

Active Publication Date: 2013-10-23
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the large diameter of silica nanospheres (500nm), and then coated with a 200nm thick silver film, the radius of curvature of the silver nanoshell is relatively large (450nm), and the SERS

Method used

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  • Analysis and detection method of surface enhanced Raman of benzo (a) pyrene
  • Analysis and detection method of surface enhanced Raman of benzo (a) pyrene
  • Analysis and detection method of surface enhanced Raman of benzo (a) pyrene

Examples

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Example Embodiment

[0033] Example 1

[0034] 1) Synthesize gold nanoparticles with a diameter of 55±10nm:

[0035] Take 200 mL of 0.01% chloroauric acid aqueous solution in a 250 mL round-bottomed flask, heat to boiling under reflux under magnetic stirring, and then quickly add 1.4 mL of 1% sodium citrate aqueous solution, the solution will change in half a minute If it is black, continue to heat under reflux for 40 minutes, the solution will gradually change from light yellow to brown-red. After it is completely reacted, it will be naturally cooled to room temperature to obtain a gold nanoparticle sol with a diameter of about 55±10nm (see figure 1 ).

[0036] 2) Self-assembly of gold nanoparticles into SERS active substrate:

[0037] The gold sol synthesized in 1) was concentrated by centrifugal separation, and washed with ultrapure water and centrifuged once (the centrifugal conditions were 8000r / min, 10min). Take 1mL of the concentrated gold nanoparticle solution in a clean small crucible, and then ...

Example Embodiment

[0041] Example 2

[0042] 1) Synthesize gold nanoparticles with a diameter of 55±10nm:

[0043] Take 200 mL of 0.01% chloroauric acid aqueous solution in a 250 mL round-bottomed flask, heat to boiling under reflux under magnetic stirring, and then quickly add 1.4 mL of 1% sodium citrate aqueous solution, the solution will change in half a minute If it is black, continue heating under reflux for 40 minutes, and the solution will gradually change from light yellow to brownish red. After it is completely reacted, it will be naturally cooled to room temperature to obtain a gold nanoparticle sol with a diameter of about 55±10nm.

[0044] 2) Self-assembly of gold nanoparticles into SERS active substrate:

[0045] The gold sol synthesized in 1) was concentrated by centrifugal separation, and washed with ultrapure water and centrifuged once (the centrifugal conditions were 8000r / min, 10min). Take 1mL of the concentrated gold nanoparticle solution in a clean small crucible, and then add 200μL...

Example Embodiment

[0051] Example 3

[0052] 1) Synthesize gold nanoparticles with a diameter of 55±10nm:

[0053] Take 200 mL of 0.01% chloroauric acid aqueous solution in a 250 mL round-bottomed flask, heat to boiling under reflux under magnetic stirring, and then quickly add 1.4 mL of 1% sodium citrate aqueous solution, the solution will change in half a minute If it is black, continue heating under reflux for 40 minutes, and the solution will gradually change from light yellow to brownish red. After it is completely reacted, it will be naturally cooled to room temperature to obtain a gold nanoparticle sol with a diameter of about 55±10nm.

[0054] 2) Self-assembly of gold nanoparticles into SERS active substrate:

[0055] The gold sol synthesized in 1) was concentrated by centrifugal separation, and washed with ultrapure water and centrifuged once (the centrifugal conditions were 8000r / min, 10min). Take 1mL of the concentrated gold nanoparticle solution into four clean small crucibles, and then add...

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Abstract

The invention discloses an analysis and detection method of surface enhanced Raman of benzo (a) pyrene. The method comprises the following steps that: firstly, gold nanoparticles are synthesized in a solution phase, are decorated by thiol and automatically assemble to form a large-area gold nanoparticle film in a gas/liquid interface, the large-area gold nanoparticle film is transferred to a silicon wafer to serve as an SERS (Surface Enhanced Raman Scattering) substrate, and the hydrophobic carbon chain end of the thiol can capture benzo (a) pyrene molecules to locate the benzo (a) pyrene molecules in the effective range of a plasma resonance magnetic field of the gold nanoparticles so as to analyze and detect the surface enhanced Raman of the benzo (a) pyrene. The particle diameters of the synthesized gold nanoparticles are uniformly distributed, the structure of a single layer of nanoparticles assembled in the gas/liquid interface is regular and orderly, and the nanoparticles can realize quantitative analysis on the benzo (a) pyrene if being taken as the SERS substrate. The method is simple in substrate preparation, short in sample analysis time and capable of realizing on-line detection through portable Raman, and the substrate can be reused.

Description

technical field [0001] The invention relates to the detection of surface-enhanced Raman, in particular to a method for quantitative analysis and detection of benzo(a)pyrene on a large-area and regular SERS substrate prepared by self-assembly of a gas-liquid interface. Background technique [0002] Benzo(a)pyrene is a fused-ring aromatic hydrocarbon found in coal tar, which can be found in vehicle exhaust (especially diesel engines), tobacco and wood smoke, and char-grilled foods. Because these substances are fat-soluble and almost impossible to degrade naturally, once they pollute the air, water, soil and other natural environments, they can accumulate to harmful concentrations at extremely low levels, and after being ingested by the human body directly or through the food chain, they can be degraded. Induce skin, lung, and digestive tract cancers through skin, respiratory tract, and digestive tract (Jia Tao. Environmental pollution is much more harmful than smoking—from ben...

Claims

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Application Information

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IPC IPC(8): G01N21/65
Inventor 张芹郭伟黄志勇
Owner JIMEI UNIV
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