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Analysis and detection method of surface enhanced Raman of benzo (a) pyrene

A surface-enhanced Raman and detection method technology, applied in Raman scattering, material excitation analysis, etc., can solve the problems of weakened SERS enhancement effect, easy oxidation, unstable silver film, etc., and achieve simple production, uniform particle size, Prepare simple effects

Active Publication Date: 2013-10-23
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the large diameter of silica nanospheres (500nm), and then coated with a 200nm thick silver film, the radius of curvature of the silver nanoshell is relatively large (450nm), and the SERS enhancement effect will be relatively weakened.
At the same time, the silver film is unstable in the air and is prone to oxidation, and the silver plating itself also needs a large vacuum coating machine

Method used

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  • Analysis and detection method of surface enhanced Raman of benzo (a) pyrene
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  • Analysis and detection method of surface enhanced Raman of benzo (a) pyrene

Examples

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Effect test

Embodiment 1

[0034] 1) Synthesis of gold nanoparticles with a particle size of 55±10nm:

[0035] Take 200mL of a 0.01% aqueous solution of chloroauric acid in a 250mL round bottom flask, heat it to boiling under reflux under magnetic stirring, then quickly add 1.4mL of a 1% aqueous solution of sodium citrate, and the solution will change within half a minute. Black, continue to reflux heating for 40min, the solution gradually changes from light yellow to brownish red, let it completely react and cool to room temperature naturally, then you can get a gold nanoparticle sol with a diameter of about 55 ± 10nm (see figure 1 ).

[0036] 2) Gold nanoparticles self-assemble into a SERS active substrate:

[0037] Concentrate the gold sol synthesized in 1) by centrifugation, wash and centrifuge once with ultrapure water (both centrifugation conditions are 8000r / min, 10min). Take 1mL of the concentrated gold nanoparticle solution in a clean small crucible, and then add 200 μL of 1mmol / L n-dodecanet...

Embodiment 2

[0042] 1) Synthesis of gold nanoparticles with a particle size of 55±10nm:

[0043] Take 200mL of a 0.01% aqueous solution of chloroauric acid in a 250mL round bottom flask, heat it to boiling under reflux under magnetic stirring, then quickly add 1.4mL of a 1% aqueous solution of sodium citrate, and the solution will change within half a minute. black, continue to reflux heating for 40min, the solution gradually changes from light yellow to brownish red, let it completely react and then naturally cool to room temperature to obtain a gold nanoparticle sol with a diameter of about 55 ± 10nm.

[0044] 2) Gold nanoparticles self-assemble into a SERS active substrate:

[0045] Concentrate the gold sol synthesized in 1) by centrifugation, wash and centrifuge once with ultrapure water (both centrifugation conditions are 8000r / min, 10min). Take 1mL of the concentrated gold nanoparticle solution in a clean small crucible, and then add 200 μL of 1mmol / L n-dodecanethiol-methanol soluti...

Embodiment 3

[0052] 1) Synthesis of gold nanoparticles with a particle size of 55±10nm:

[0053] Take 200mL of a 0.01% aqueous solution of chloroauric acid in a 250mL round bottom flask, heat it to boiling under reflux under magnetic stirring, then quickly add 1.4mL of a 1% aqueous solution of sodium citrate, and the solution will change within half a minute. black, continue to reflux heating for 40min, the solution gradually changes from light yellow to brownish red, let it completely react and then naturally cool to room temperature to obtain a gold nanoparticle sol with a diameter of about 55 ± 10nm.

[0054] 2) Gold nanoparticles self-assemble into a SERS active substrate:

[0055] Concentrate the gold sol synthesized in 1) by centrifugation, wash and centrifuge once with ultrapure water (both centrifugation conditions are 8000r / min, 10min). Take 1 mL of the concentrated gold nanoparticle solution in four clean small crucibles, and then add 100 μL, 200 μL, 300 μL, and 400 μL of 1 mmol...

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Abstract

The invention discloses an analysis and detection method of surface enhanced Raman of benzo (a) pyrene. The method comprises the following steps that: firstly, gold nanoparticles are synthesized in a solution phase, are decorated by thiol and automatically assemble to form a large-area gold nanoparticle film in a gas / liquid interface, the large-area gold nanoparticle film is transferred to a silicon wafer to serve as an SERS (Surface Enhanced Raman Scattering) substrate, and the hydrophobic carbon chain end of the thiol can capture benzo (a) pyrene molecules to locate the benzo (a) pyrene molecules in the effective range of a plasma resonance magnetic field of the gold nanoparticles so as to analyze and detect the surface enhanced Raman of the benzo (a) pyrene. The particle diameters of the synthesized gold nanoparticles are uniformly distributed, the structure of a single layer of nanoparticles assembled in the gas / liquid interface is regular and orderly, and the nanoparticles can realize quantitative analysis on the benzo (a) pyrene if being taken as the SERS substrate. The method is simple in substrate preparation, short in sample analysis time and capable of realizing on-line detection through portable Raman, and the substrate can be reused.

Description

technical field [0001] The invention relates to the detection of surface-enhanced Raman, in particular to a method for quantitative analysis and detection of benzo(a)pyrene on a large-area and regular SERS substrate prepared by self-assembly of a gas-liquid interface. Background technique [0002] Benzo(a)pyrene is a fused-ring aromatic hydrocarbon found in coal tar, which can be found in vehicle exhaust (especially diesel engines), tobacco and wood smoke, and char-grilled foods. Because these substances are fat-soluble and almost impossible to degrade naturally, once they pollute the air, water, soil and other natural environments, they can accumulate to harmful concentrations at extremely low levels, and after being ingested by the human body directly or through the food chain, they can be degraded. Induce skin, lung, and digestive tract cancers through skin, respiratory tract, and digestive tract (Jia Tao. Environmental pollution is much more harmful than smoking—from ben...

Claims

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Application Information

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IPC IPC(8): G01N21/65
Inventor 张芹郭伟黄志勇
Owner JIMEI UNIV
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