Beta-elemene synthetase, encoding gene thereof, carrier, engineering bacterium and application of beta-elemene synthetase

Abstract

The invention provides a beta-elemene synthetase, an encoding gene thereof, a carrier with the encoding gene, an engineering bacterium with the encoding gene and an application of the beta-elemene synthetase. The beta-elemene synthetase has an amino acid sequence as shown in SEQ ID No.1, and the encoding gene of the beta-elemene synthetase has a sequence as shown in SEQ ID No.2. The invention provides the beta-elemene synthetase and the encoding gene thereof and provides the basis for producing the beta-elemene synthetase by using a biological enzyme method; the quality of a beta-elemene synthetase product can be improved, the production cost of the beta-elemene synthetase can be reduced, the production efficiency can be increased, and the technical precondition is provided for the industrial biosynthesis of the beta-elemene synthetase.

Description

(1) Technical field

[0001] The invention relates to a β-elemene synthetase, a gene encoding the enzyme, a recombinant vector containing the encoding gene, a genetically engineered bacterium and applications thereof. (2) Background technology

[0002] β-elemene (β-elemene) belongs to the oxygen-free sesquiolefins. It is an oily monomer separated from the volatile oil of zedoary. Its chemical name is 1-methyl-1-vinyl-2. 4-diisopropylcyclohexane, the structural formula is shown in Formula 1. β-elemene has been proven to have anti-tumor activity against adenocarcinoma, human neurokeratinoma, liver cancer, lung cancer, colon cancer, gastric cancer, ovarian cancer, etc. In addition, some studies have also shown that β-elemene also has analgesic and Radiation sensitization. Elemene milk with β-elemene as the main component began to be widely used in the clinical treatment of malignant serous cavity effusion, lung cancer, digestive tract tumors, brain tumors and other superficial ...

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