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Organic phosphorus pesticide inspection method for treating biological detection material by using gel permeation chromatography

A technology of gel permeation chromatography and organophosphorus pesticides, which is applied in the field of criminal investigation, can solve the problems of shortening the pretreatment time, analysis cost, complex components, etc., and achieves the effects of shortening the pretreatment time, high sensitivity, and reducing the analysis cost.

Active Publication Date: 2015-03-25
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The beneficial effects of the present invention are: the present invention applies the gel permeation chromatography technology to the field of poison analysis, establishes a simple, fast, sensitive and high recovery pretreatment technology in blood, urine and liver tissue, shortens the pretreatment time, reduces Analyze the cost and improve the reliability of the test results to solve the problem of a wide variety of test materials and complex components in toxicant analysis, and provide a guarantee for improving the efficiency of toxicant analysis and inspection work in the public security industry

Method used

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  • Organic phosphorus pesticide inspection method for treating biological detection material by using gel permeation chromatography
  • Organic phosphorus pesticide inspection method for treating biological detection material by using gel permeation chromatography
  • Organic phosphorus pesticide inspection method for treating biological detection material by using gel permeation chromatography

Examples

Experimental program
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Effect test

Embodiment 1

[0027]Embodiment 1: one, the preparation of standard solution

[0028] 1. Preparation of standard stock solution: Weigh 10 mg each of dimethoate, omethoate, malathion, parathion, methyl parathion, phorate, fenthion and diphorate standard respectively, respectively Place in a 10mL volumetric flask, add a small amount of n-hexane to dissolve and set the volume to the mark, and prepare dimethoate, omethoate, malathion, parathion, methyl parathion, Standard stock solutions of phorate, fenthion and diphorate, stored in a refrigerator at 4°C, for later use.

[0029] Pipette a certain amount of single standard stock solution respectively to prepare 8 kinds of organophosphorus pesticide mixed standard solution (that is, the solutes in the 8 kinds of organophosphorus pesticide mixed standard solution are dimethoate, omethoate, malathion, parathion Phosphorus, methyl parathion, phorate, fenthion and diphorate).

[0030] Pipette the single standard stock solution of a certain amount of...

Embodiment 2

[0090] Embodiment 2: The difference between this embodiment and Example 1 is: the extraction solvent (i.e. organic solvent A) is a mixed solution of cycloheptane and ethylene glycol diethyl ether, wherein the volume ratio of cycloheptane and ethylene glycol diethyl ether The ratio is 18:3; other experimental conditions are the same; when the mixed solution of cycloheptane and ethylene glycol diethyl ether is used as the extraction solvent, the addition recoveries and precisions of the eight organophosphorus pesticides are shown in Table 1-10~1-12 shown.

[0091] Table 1-10 Addition recovery and precision of 8 organophosphorus pesticides in blood after GPC purification

[0092]

[0093] Table 1-11 Addition recovery and precision of 8 organophosphorus pesticides in urine after GPC purification

[0094]

[0095] Table 1-12 Addition recovery and precision of 8 organophosphorus pesticides in liver after GPC purification

[0096]

[0097]

[0098] It can be seen from Tab...

Embodiment 3

[0099] Embodiment 3: The difference between this embodiment and Example 1 is: the extraction solvent (i.e. organic solvent A) is a mixed solution of isoamyl ether and propyl butyrate, wherein the volume ratio of isoamyl ether and propyl butyrate is 1 : 2.5; other experimental conditions are the same; when the mixed solution of isoamyl ether and propyl butyrate is used as the extraction solvent, the addition recoveries and precisions of 8 kinds of organophosphorus pesticides are shown in Table 1-13~1-15.

[0100] Table 1-13 Addition recovery and precision of 8 organophosphorus pesticides in blood after GPC purification

[0101]

[0102] Table 1-14 Addition recovery and precision of 8 organophosphorus pesticides in urine after GPC purification

[0103]

[0104]

[0105] Table 1-15 Addition recovery and precision of 8 organophosphorus pesticides in liver after GPC purification

[0106]

[0107] As can be seen from Tables 1-13 to 1-15, using the mixed solution of isoa...

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Abstract

The present invention discloses an organic phosphorus pesticide inspection method for treating a biological detection material by using a gel permeation chromatography. The method comprises the following steps: (1) adding an organic solvent A to a biological detection material, carrying out centrifugation, separating the organic phase, concentrating the obtained organic phase until drying, dissolving with the organic solvent A, and filtering with an organic microporous filtration membrane to obtain the extraction solution; (2) adding the extraction solution to a gel permeation chromatography purification column, carrying out elution with an organic solvent B, collecting the eluent, carrying out blowing drying through a concentrator, and dissolving with an organic solvent C to obtain a solution requiring detection, wherein the filling phase of the gel permeation chromatography purification column is a styrene-divinylbenzene copolymer; and (3) detecting the solution requiring detection by using a gas chromatography. According to the present invention, the gel permeation chromatography technology is applied in the field of toxicant analysis, the biological detection material pretreatment technology with characteristics of simpleness, rapidness, high sensitivity and high recovery rate is established based on the organic phosphorus pesticide, advantages of pretreatment time shortening, analysis cost reduction and detection result reliability increase are provided, problems of various detection material types and complex components in the toxicant analysis are solved, and assurance is provided for improvement of efficiency of the toxicant analysis inspection work in the public security industry.

Description

technical field [0001] The invention relates to a drug and poison detection method in the field of criminal investigation, in particular to an organophosphorus pesticide detection method using gel permeation chromatography to process biological inspection materials. Background technique [0002] With the advancement of technology, the requirements for drug and toxicant analysis and testing are getting higher and higher, including high reproducibility, high recovery rate, low detection limit and simple operation, etc., so it is urgent to establish a simple, fast and sensitive and a pretreatment method with a high recovery rate. At present, the existing pretreatment technologies mainly include liquid-liquid extraction, solid-phase extraction, and solid-phase microextraction. Check the situation. [0003] At present, most of the gel permeation chromatography researches at home and abroad focus on the processing of plant products such as vegetables, fruits, and grains. Since ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/14G01N30/02
Inventor 栾玉静董颖王瑞花常靖杜鸿雁张蕾萍于忠山王炯张云峰王芳琳何毅侯小平崔冠峰
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY