Method for preparing active factor sustained-releasing PELA (Percutaneous endovenous laser ablation) microsphere scaffold bone repairing material
An active factor, PLA-PEG-PLA technology, applied in medical science, prosthesis, etc., can solve the problems of high brittleness, strong bone conduction ability, and inability to release strong chemotaxis-induced osteogenesis in a sustained manner, and achieve tissue phase Good capacity, simple preparation, no immunogenic effect
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[0037] Example one
[0038] 1. Select capsule material
[0039] The biodegradable PLA-PEG-PLA triblock copolymer is used as the basic capsule material, and the molecular weight of the high molecular polymer PEG in the triblock copolymer accounts for 10% of the total polymer molecular weight;
[0040] 2. Preparation of PELA / rhBMP-7 initial drug-loaded microcapsules
[0041] First, add 1ug of rhBMP-7 dry powder to 150ul of distilled water to dissolve and mix to form an internal aqueous phase; then, 240mg of PLA-PEG-PLA triblock copolymer with a total molecular weight of 20000Da is placed in a second containing 0.5% span-20 4ml of methyl chloride organic solvent is made into oil phase; then the prepared inner water phase is added to the oil phase for ice bath ultrasonic emulsification to form colostrum; finally, the colostrum is added to the outer water containing 0.5% PVA (polyvinyl alcohol) In the phase, stir at 800 rpm for 30 minutes, remove the organic solvent, centrifuge, wash with...
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[0047] Example two
[0048] 1. Select capsule material
[0049] The biodegradable PLA-PEG-PLA triblock copolymer is used as the basic capsule material, and the molecular weight of the high molecular polymer PEG in the triblock copolymer accounts for 20% of the total polymer molecular weight;
[0050] 2. Preparation of PELA / rhBMP-7 initial drug-loaded microcapsules
[0051] First, add 1ug of rhBMP-7 dry powder to 200ul of distilled water to dissolve and mix uniformly to form an internal aqueous phase; then, 280mg of PLA-PEG-PLA triblock copolymer with a total molecular weight of 20000Da is placed in the second containing 0.5% span-20 4ml of methyl chloride organic solvent is made into oil phase; then the prepared inner water phase is added to the oil phase for ice bath ultrasonic emulsification to form colostrum; finally, the colostrum is added to the outer water containing 0.5% PVA (polyvinyl alcohol) In the phase, stir at 800rpm for 30 minutes, remove the organic solvent, centrifuge...
Example Embodiment
[0056] Example three
[0057] 1. Select capsule material
[0058] The biodegradable PLA-PEG-PLA triblock copolymer is used as the basic capsule material, and the molecular weight of the high molecular polymer PEG in the triblock copolymer accounts for 30% of the total polymer molecular weight;
[0059] 2. Preparation of PELA / rhBMP-7 initial drug-loaded microcapsules
[0060] First, add 1ug of rhBMP-7 dry powder to 250ul of distilled water to dissolve and mix uniformly to form an internal aqueous phase; then, 320mg of PLA-PEG-PLA triblock copolymer with a total molecular weight of 20000Da is placed in a second containing 0.5% span-20 4ml of methyl chloride organic solvent is made into oil phase; then the prepared inner water phase is added to the oil phase for ice bath ultrasonic emulsification to form colostrum; finally, the colostrum is added to the outer water containing 0.5% PVA (polyvinyl alcohol) In the phase, stir at 800 rpm for 30 minutes, remove the organic solvent, centrifug...
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