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Method for preparing active factor sustained-releasing PELA (Percutaneous endovenous laser ablation) microsphere scaffold bone repairing material

An active factor, PLA-PEG-PLA technology, applied in medical science, prosthesis, etc., can solve the problems of high brittleness, strong bone conduction ability, and inability to release strong chemotaxis-induced osteogenesis in a sustained manner, and achieve tissue phase Good capacity, simple preparation, no immunogenic effect

Inactive Publication Date: 2014-03-05
李夏林 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of these bone graft materials are inorganic materials with high brittleness, only some of them have strong bone conduction ability, and cannot release bone growth factors slowly to produce strong chemotaxis and osteogenesis

Method used

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  • Method for preparing active factor sustained-releasing PELA (Percutaneous endovenous laser ablation) microsphere scaffold bone repairing material

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Select capsule material

[0039] The biodegradable PLA-PEG-PLA tri-block copolymer is used as the basic capsule material, and the molecular weight of the polymer PEG in the tri-block copolymer accounts for 10% of the total polymer molecular weight;

[0040] 2. Preparation of PELA / rhBMP-7 initial drug-loaded microcapsules

[0041] First, 1ug ​​of rhBMP-7 dry powder was dissolved in 150ul distilled water, and mixed uniformly to form an inner aqueous phase; then, 240mg of PLA-PEG-PLA triblock copolymer with a total molecular weight of 20,000Da was placed in distilled water containing 0.5% span-20 Make an oil phase with 4ml of methyl chloride organic solvent; then add the prepared inner water phase into the oil phase for ultrasonic emulsification in an ice bath to form colostrum; finally, add the colostrum to the outer water containing 0.5% PVA (polyvinyl alcohol) In the phase, stir at 800 rpm for 30 minutes, remove the organic solvent, centrifuge, wash with distilled w...

Embodiment 2

[0048] 1. Select capsule material

[0049] The biodegradable PLA-PEG-PLA tri-block copolymer is used as the basic capsule material, and the molecular weight of the polymer PEG in the tri-block copolymer accounts for 20% of the total polymer molecular weight;

[0050] 2. Preparation of PELA / rhBMP-7 initial drug-loaded microcapsules

[0051] First, 1ug ​​of rhBMP-7 dry powder was dissolved in 200ul distilled water, and mixed uniformly to form an inner aqueous phase; then, 280mg of PLA-PEG-PLA triblock copolymer with a total molecular weight of 20000Da was placed in a distilled water solution containing 0.5% span-20 Make an oil phase with 4ml of methyl chloride organic solvent; then add the prepared inner water phase into the oil phase for ultrasonic emulsification in an ice bath to form colostrum; finally, add the colostrum to the outer water containing 0.5% PVA (polyvinyl alcohol) In the phase, stir at 800rpm for 30 minutes, remove the organic solvent, centrifuge, wash with di...

Embodiment 3

[0057] 1. Select capsule material

[0058] The biodegradable PLA-PEG-PLA tri-block copolymer is used as the basic capsule material, and the molecular weight of the polymer PEG in the tri-block copolymer accounts for 30% of the total polymer molecular weight;

[0059] 2. Preparation of PELA / rhBMP-7 initial drug-loaded microcapsules

[0060] First, 1ug ​​of rhBMP-7 dry powder was dissolved in 250ul of distilled water, and mixed uniformly to form an inner aqueous phase; then, 320mg of PLA-PEG-PLA triblock copolymer with a total molecular weight of 20,000Da was placed in distilled water containing 0.5% span-20 Make an oil phase with 4ml of methyl chloride organic solvent; then add the prepared inner water phase into the oil phase for ultrasonic emulsification in an ice bath to form colostrum; finally, add the colostrum to the outer water containing 0.5% PVA (polyvinyl alcohol) In the phase, stir at 800rpm for 30 minutes, remove the organic solvent, centrifuge, wash with distilled...

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Abstract

The invention discloses a method for preparing an active factor sustained-releasing PELA (Percutaneous endovenous laser ablation) microsphere scaffold bone repairing material. The method comprises the following steps of selecting biodegradable PELA as a basic capsule wall material; mixing rhBMP-7 dry powder and distilled water, and dissolving the rhBMP-7 dry powder to form an internal aqueous phase; uniformly mixing the PELA and an organic solvent dichloromethane to form an oil phase; adding the internal aqueous phase into the oil phase, and performing ice-bath ultrasonic emulsification to form primary emulsion; adding the primary emulsion into external aqueous phase, performing stirring to remove the organic solvent, performing repeated washing and centrifugation operation, and performing vacuum freeze-drying to form initial micro-capsule dry powder for storage; adding dissolution fluid into the initial micro-capsule dry powder for uniform mixing; freeze-drying the initial micro-capsule dry powder mixed with BMP-2 to form final micro-capsule dry powder; placing the final micro-capsule dry powder into a die; placing the die into a sealed container with dichloromethane, and performing sealed steam fumigation until the dry powder is gradually fused into a viscous and elastic body with certain porosity; air-drying the viscous and elastic body to completely volatilize the dichloromethane remaining the surface of the material to obtain a filling material for bone defects.

Description

technical field [0001] The invention relates to a preparation method of a microsphere scaffold bone repair material, in particular, to a method that can imitate the natural fracture healing process and sequentially release two major osteogenesis-inducing active factors, in order to stimulate and induce osteogenesis to the maximum within a safe dosage range. The preparation method of the formed sustained-release active factor PELA microsphere scaffold bone repair material. Background technique [0002] Large bone defects are still a thorny problem that clinicians often need to face. According to statistics, there are as many as 3 million patients in China who have bone defects and dysfunction due to various reasons every year. Bone transplantation has become the second most demanded graft, second only to blood transfusion. Artificial bone research in recent decades has provided a promising solution to this problem. Because it can be artificially synthesized, has a wide rang...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/54
Inventor 李夏林靳安民闵少雄
Owner 李夏林
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