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Antimicrobial bacterial cellulose and preparation method thereof

A bacterial cellulose, unsaturated technology, used in medical science, prosthesis and other directions, can solve the problems of low efficiency and stability of bactericide adsorption, bacterial cellulose does not have antibacterial function, harmful microorganism pollution, etc., to achieve high efficiency and long-lasting Bactericidal effect, high-efficiency and long-lasting antibacterial effect, long-lasting antibacterial effect

Active Publication Date: 2014-04-16
SHENZHEN INST OF ADVANCED TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, bacterial cellulose itself does not have antibacterial function, and it is easily polluted by harmful microorganisms in the air or water, causing a series of infection problems
[0003] In order to make bacterial cellulose have antibacterial function, the bacterial cellulose is placed in the fungicide solution by soaking and adsorbing, but the adsorption efficiency and stability of the fungicide are not high, and the bactericidal effect is poor.

Method used

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  • Antimicrobial bacterial cellulose and preparation method thereof
  • Antimicrobial bacterial cellulose and preparation method thereof
  • Antimicrobial bacterial cellulose and preparation method thereof

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[0029] First aspect, the present invention provides a kind of preparation method of antibacterial bacterial cellulose, comprises the following steps:

[0030] (1) Provide bacterial cellulose;

[0031] (2) Soak the bacterial cellulose in an unsaturated carboxylic acid, then add a polymerization inhibitor to obtain a mixed solution, add a catalyst to the mixed solution, and conduct an esterification reaction at room temperature for 1 to 4 hours to obtain a mixed solution containing Unsaturated bond esterified bacterial cellulose solution, after purification, to obtain dry esterified bacterial cellulose containing unsaturated bonds;

[0032] (3) After soaking the dried esterified bacterial cellulose containing unsaturated bonds in deionized water, under an inert atmosphere, add an unsaturated quaternary ammonium salt monomer and an initiator to obtain a reaction solution, and the reaction solution is Addition reaction and polymerization reaction are carried out at 50°C to 60°C f...

Embodiment 1

[0071] (1) Inoculate 1mL of Acetobacter xylinum into 100mL of culture medium, culture statically at 30°C until a bacterial cellulose film with a thickness of 5mm is obtained, and place the bacterial cellulose film in a sodium hydroxide solution with a concentration of 0.1mol / L. Shake and break the cells at 80°C for 30 minutes. After repeated three times, use deionized water to wash away the broken cells and sodium hydroxide until the surface pH of the bacterial cellulose membrane is neutral. After steam sterilization, freeze-dry for later use; the culture medium The ingredients are: 0.1g / mL glucose, 0.03g / mL peptone, 0.02g / mL yeast powder, 0.01g / mL disodium hydrogen phosphate, 0.005g / mL magnesium sulfate, 0.005g / mL ammonium sulfate, 0.005mL / mL corn syrup extract;

[0072] (2) Take 0.5 g of the bacterial cellulose membrane obtained in step (1), soak it in 20 mL of methacrylic acid for 2 hours, and add a polymerization inhibitor terephthalic acid whose mass is 0.1% of the total...

Embodiment 2

[0079](1) Inoculate 1mL of Acetobacter xylinum into 100mL of culture medium, culture statically at 30°C until a bacterial cellulose film with a thickness of 5mm is obtained, and place the bacterial cellulose film in a sodium hydroxide solution with a concentration of 0.1mol / L. Shaking and breaking the cells at 80°C for 30 minutes, repeated three times, washing the broken cells and sodium hydroxide with deionized water until the surface pH of the bacterial cellulose membrane was neutral, steam sterilized, freeze-dried for later use; The composition of the culture medium is: 0.4g / mL glucose, 0.06g / mL peptone, 0.03g / mL yeast powder, 0.02g / mL disodium hydrogen phosphate, 0.015g / mL magnesium sulfate, 0.01g / mL ammonium sulfate, 0.015mL / mL corn syrup extract;

[0080] (2) Take 0.4 g of the bacterial cellulose membrane obtained in step (1), soak it in 20ml of acrylic acid for 1 hour, add a polymerization inhibitor hydroquinone whose mass is 0.2% of the total mass of the bacterial cell...

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Abstract

The invention provides antimicrobial bacterial cellulose and a preparation method thereof. According to the antimicrobial bacterial cellulose provided by the invention, a large number of hydroxyl functional groups existing on the bacterial cellulose are utilized, a quaternary ammonium salt antimicrobial group is introduced by surface modification, the structure of the bacterial cellulose is not damaged, the shape of hydrated gel is maintained, the effect of promoting healing of a wound is realized, and the antimicrobial bacterial cellulose prepared by the preparation method provided by the invention simultaneously has the advantages of good chemical stability and lasting antimicrobial effect.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to an antibacterial bacterial cellulose and a preparation method thereof. Background technique [0002] Bacterial cellulose is used as an alternative material for artificial skin or wound repair. Compared with other materials such as collagen, hyaluronic acid and silica gel, bacterial cellulose has a network structure and mechanical support similar to skin, and does not require The complex chemical reaction only requires simple bacterial culture to form a hydrated gel with a controllable shape. It has low immune rejection and can well promote wound healing. It is a good wound repair material. However, bacterial cellulose itself does not have antibacterial function, and it is easily polluted by harmful microorganisms in the air or water, thus causing a series of infection problems. [0003] In order to make the bacterial cellulose possess the antibacterial f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F290/10C08F220/34C08F226/02C08F4/40C08B3/08A61L27/20A61L27/52A61L27/54A61L27/60
Inventor 王金慧周阳胡阳周新朱勇军潘浩波
Owner SHENZHEN INST OF ADVANCED TECH
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