Preparation method of crosslinked chitosan/gelatin composite porous scaffold

A technology of cross-linking chitosan and porous scaffolds, which is applied in medical science, prosthesis and other directions, can solve problems such as weak mechanical strength, and achieve the effects of good mechanical properties, good affinity, and simple and convenient process

Inactive Publication Date: 2014-04-16
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Based on the advantages of convenience, easy access, taking from nature and using in nature, taking advantage of the similarity and compatibility between natural biological materials and human tissues, many researchers have chosen natural biological materials to prepare tissue engineering scaffold materials, but

Method used

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  • Preparation method of crosslinked chitosan/gelatin composite porous scaffold
  • Preparation method of crosslinked chitosan/gelatin composite porous scaffold
  • Preparation method of crosslinked chitosan/gelatin composite porous scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The methanol suspension of 2% chitosan, the methanol suspension of gelatin and the methanol solution of silane coupling agent KH-560 were configured respectively in mass percentage;

[0024] Blend the above methanol suspension of chitosan, gelatin in methanol and silane coupling agent KH-560 in methanol at a mass ratio of 35:35:30, heat up to 70°C, stir for 3 hours, and filter with suction , repeatedly washing with anhydrous methanol until the unreacted silane coupling agent KH-560 is washed away, and after vacuum drying at 60°C for 24 hours, a cross-linked chitosan / gelatin composite material is obtained;

[0025] With above-mentioned cross-linked chitosan / gelatin composite material, be dissolved in 100g distilled water, prepare the aqueous solution that mass percent is 1% cross-linked chitosan / gelatin composite material, defoam, obtain the cross-linked chitosan after deaeration Aqueous solutions of sugar / gelatin composites;

[0026] Pour the above degassed cross-linke...

Embodiment 2

[0029] Same as Example 1, but the methanol suspension of chitosan, the methanol suspension of gelatin and the concentration of the methanol solution of silane coupling agent KH-560 in step (1) are all changed from 2% to 3%, other unchanged .

Embodiment 3

[0031] Same as Example 1, but the methanol suspension of chitosan, the methanol suspension of gelatin and the concentration of the methanol solution of silane coupling agent KH-560 in step (1) are all changed from 2% to 1%, and others remain unchanged .

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Abstract

The invention belongs to the technical field of new materials, and particularly relates to a preparation method of a crosslinked chitosan/gelatin composite porous scaffold. According to the method, chitosan, gelatin and other natural biological materials are used as raw materials, an organosilane coupling agent is used as a crosslinking agent, and a freeze-drying process is utilized to prepare the excellent-mechanical-property crosslinked chitosan/gelatin composite porous scaffold composed of pure natural biological materials. The crosslinked chitosan/gelatin composite porous scaffold has the characteristics of high porosity, high pore strength, large pore diameter and the like; the pore size is 100-300 mu m, and the porosity is 90%; and the crosslinked chitosan/gelatin composite porous scaffold is in an open pore structure in which larger pores contains smaller pores, and thus, cells are suitable to be implanted on the scaffold surface. Compared with the chitosan scaffold, the crosslinked chitosan/gelatin composite porous scaffold has better mechanical properties; and the addition of the gelatin and organosilicon crosslinking agent is more beneficial to proliferation and differentiation of cells on the porous scaffold surface, and the crosslinked chitosan/gelatin composite porous scaffold has higher affinity for biological cells.

Description

technical field [0001] The invention belongs to the technical field of new materials, and in particular relates to a preparation method of a cross-linked chitosan / gelatin composite porous support. Background technique [0002] The emergence of tissue engineering has brought bright prospects for human health and life extension. The use of biomedical materials to prepare artificial substitutes to treat diseases can solve the problem that human organs need to be replaced due to defects or loss of function, but the materials are limited. When people put their energy into artificial tissues or organs, one of the keys is the preparation of three-dimensional porous scaffold materials for tissue engineering using cell culture. [0003] At present, porous scaffold materials for tissue engineering are mainly derived from some natural polymers and synthetic polymers. Natural polymers mainly include collagen, chitin, chitosan, hyaluronic acid, gelatin, alginate, chondroitin sulfate, et...

Claims

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Application Information

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IPC IPC(8): C08J9/28C08J3/24C08L5/08C08L89/00C08K5/5435A61L27/24A61L27/20A61L27/56
Inventor 杨正龙
Owner TONGJI UNIV
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