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A kind of microbial fermentation agent for treating urban sludge and preparation method thereof

A technology of microbial starter and municipal sludge, which is applied in the field of fermenting organisms to achieve the effects of thorough fermentation, improving work efficiency, and improving the activity of microorganisms and enzymes

Inactive Publication Date: 2016-06-01
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using biological fermentation to solve urban sludge is a feasible and non-polluting method, but there is no fermentation agent specifically for urban sludge

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0020] A preparation method for treating municipal sludge with microbial fermentation agent, comprising the following steps:

[0021] Step 1, preparation of soybean extract

[0022] (1) Wash the soybeans, dry them, crush them, and then filter them through a 1mm-diameter sieve to obtain soybean powder for later use;

[0023] (2) Add soybean powder into a phosphate buffer solution with a pH of 5.2 and a molar concentration of 0.05 mol / L, stir evenly to obtain a mixed solution I, and the volume of the phosphate buffer solution corresponding to each gram of soybean powder is 10 mL;

[0024] (3) Put the mixture I prepared in step (2) into a centrifuge with a rotation speed of 6000r / min for 30min, and then dissolve the precipitate in a phosphate buffer solution with a pH of 6.5 and a molar concentration of 0.05mol / L and then transfer to a centrifuge with a rotating speed of 4000r / min for 15min, collect the supernatant of twice centrifugation, and obtain a soybean extract for subseq...

Embodiment 1

[0029] A microbial fermentation agent for treating municipal sludge, said microbial fermentation agent is composed of soybean extract, Bacillus subtilis, silicate bacteria, Trichoderma viride, Lactococcus lactis, actinomycetes, Bacillus colloidus, Flavobacterium oryzae , saccharomyces, nitrogen-fixing bacteria torus, cellulase, protease and phosphorylase, and the parts by weight of each raw material required for the preparation of the starter are respectively 20 parts of soybean extract, 1 part of Bacillus subtilis, silicate 3 parts of bacteria, 2 parts of Trichoderma viridans, 8 parts of Lactococcus lactis, 2 parts of Actinomycetes, 1 part of Bacillus colloidus, 2 parts of Flavobacterium oryzae, 3 parts of yeast, 10 parts of Azotobacter torus, cellulase 8 parts, 8 parts of protease and 5 parts of phosphorylase.

[0030] A preparation method for treating municipal sludge with microbial fermentation agent, comprising the following steps:

[0031] Step 1, preparation of soybean...

Embodiment 2

[0039] A microbial fermentation agent for treating municipal sludge, said microbial fermentation agent is composed of soybean extract, Bacillus subtilis, silicate bacteria, Trichoderma viride, Lactococcus lactis, actinomycetes, Bacillus colloidus, Flavobacterium oryzae , saccharomyces, nitrogen-fixing bacteria torus, cellulase, protease and phosphorylase, and the parts by weight of each raw material required for the preparation of the starter are respectively 25 parts of soybean extract, 2 parts of Bacillus subtilis, silicate 4 parts of bacteria, 4 parts of Trichoderma viride, 12 parts of Lactococcus lactis, 4 parts of Actinomycetes, 2 parts of Bacillus colloidus, 2 parts of Flavobacterium oryzae, 6 parts of yeast, 13 parts of Azotobacter torus, cellulase 12 parts, 12 parts of protease and 6 parts of phosphorylase.

[0040] A preparation method for treating municipal sludge with microbial fermentation agent, comprising the following steps:

[0041] Step 1, preparation of soyb...

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PUM

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Abstract

The invention belongs to the technical field of fermentative organisms, and particularly relates to a microbial starter for treating municipal sludge and a preparation method thereof. By adding a soybean extract into the starter, the activity of microorganisms and enzymes in the starter is increased greatly, the temperature rising and fermentation time in a substrate fermentation process is shortened, the nutrients after fermentation are enriched, and the working efficiency is increased. The soybean extract is mixed together with complex liquid after ultrasonic treatment, so that components in the complex liquid and the soybean extract are mixed uniformly, the absorption and utilization ratios of the starter by the substrate are increased, and substrate fermentation is more complete. Moreover, ultrasonic treatment is performed intermittently, so that damages of ultrasonic waves to components in the soybean extract are prevented. A preparation process is simple, multiple nutrient substances in the municipal sludge can be decomposed in combination with multiple microorganisms and enzymes, the effect of changing waste into valuable is achieved, urban beautification is promoted, and a nutritional substrate is provided for the growth of plants.

Description

technical field [0001] The invention belongs to the technical field of fermentation biology, and in particular relates to a microbial fermentation agent for treating urban sludge and a preparation method thereof. Background technique [0002] The dewatered sludge of urban sewage plants is the solid waste generated from the treatment of wastewater in urban sewage plants. With the increase in the number of sewage plants under construction in my country, the sludge output will still increase. Municipal sludge contains a large amount of organic matter and nutrients needed by plants such as N, P, and K, which can usually be achieved by forced ventilation and aerobic static composting, but one fermentation basically takes 30 days. Therefore, it is necessary to explore an economical and effective rapid decomposing treatment method to solve the sludge in the city. [0003] Biotechnology is a new subject developed in the 20th century, and the method of using biotechnology to turn w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14C12N9/42C12N9/50C12N9/10C02F11/02C12R1/125C12R1/01C12R1/885
Inventor 常会庆徐艳珍王浩王世华
Owner HENAN UNIV OF SCI & TECH