Small peptide, synthesis method thereof, and applications of small peptide on enhancing immunity of livestock

Abstract

The invention relates to a small peptide, a synthesis method thereof, and applications of small peptide on enhancing the immunity of livestock. The small peptide is characterized in that the small peptide is a linear pentapeptide Tyr-Pro-Ile-Ser-Leu, and is synthesized by combining a step-by-step jointed chain and a segmental jointed chain. The purity of the peptide is high, the immunity action can be achieved to stimulate the immunity function of animals under extremely low added concentration, and the disease resistance of animals on epidemic diseases can be enhanced.

Description

field of invention [0001] The invention relates to an active small peptide for enhancing immunity, in particular to a small active peptide for enhancing immunity and a preparation method thereof. Background technique [0002] Peptides are linear polymers of amino acids. Those containing more than 50 amino acid residues are usually called proteins, and those with less than 50 amino acid residues are called peptides. Small peptides are also called oligopeptides, generally consisting of 2-10 amino acids. It has been confirmed that small peptides can be completely absorbed into the blood circulation and play important biological functions in the body. Animals must require a certain amount of small peptides in order to achieve optimal immune performance and production performance. In recent years, with the development of modern biotechnology, especially modern microbial fermentation technology, there are many protein-peptide-amino acid mixed products produced by hydrolyzing ani...

AI Technical Summary

Problems solved by technology

[0003] The active small peptides that enhance immunity that are currently available on the market are mostly peptide proteins processed from the small intestine and intestinal mucosa of animals or peptide protein feeds or feed additives containing a certain amount of small peptides, although they can also improve animal immunity. Strength and production performance, reduce diarrhea rate and enhance animal disease resistance, but most of them have the following defects: (1) the added amount of the product is relatively large by 0.1-1.5%; (2) the product function is not clear, and the efficacy is not obvious; (3) The molecular weight of the peptide is relatively large, generally about 6000, and it is still antigenic, which is not good for animals and can easily cause allergic reactions in animals; (4) high preparation costs or high product costs, not suitable for animal breeding

The direct enzymatic hydrolysis method uses high-priced enzymes, such as plant proteases such as papain and bromelain, or animal proteases such as trypsin and pepsin, but it is easy to produce unacceptable fishy or bitter taste, and the production cost is high. Therefore, its application in related industries is limited; although the microbial fermentation method has the advantages of wide source of raw materials, mild reaction conditions, low production cost, and environmental protection, it is difficult to extract peptides, with low yield and high cost. Its mechanism of action on the body is also difficult to explain; the second is DNA recombination technology, which has many difficulties in the operation, expression and detection of small molecule gene fragments, and it is not easy to screen highly expressed strains and the yield of strains is relatively low. Low and limited by many consumers' objections to the use of food products produced from GMOs and concerns about biosafety and biocontamination

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