Method of extracting and fermenting and separating 1,3-propylene glycol in fermentation liquor by coupling

A technology of propylene glycol and fermented liquid, which is applied in the field of bioengineering, can solve the problems of difficult recovery of raw materials, salt, and bacteria, hindering the evaporation of fractions, and small separation ability, so as to improve the utilization rate of raw materials, reduce the discharge of waste water, and have low-cost sources. Effect

Active Publication Date: 2014-08-06
DALIAN UNIV OF TECH
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve some problems in the process of the subsequent separation and purification of 1,3-propanediol produced by the current fermentation method, such as high cost, small separation capacity, difficulty in recycling raw materials, salt, and bacteria, and the late stage of vacuum distillation Problems such as salt crystallization and viscous substances hindering the evaporation of distillates provide a new method for the separation of 1,3-propanediol from microbial fermentation broths

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method of extracting and fermenting and separating 1,3-propylene glycol in fermentation liquor by coupling
  • Method of extracting and fermenting and separating 1,3-propylene glycol in fermentation liquor by coupling
  • Method of extracting and fermenting and separating 1,3-propylene glycol in fermentation liquor by coupling

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0042] 2. Preparation of 1,3-propanediol fermentation broth:

[0043] (1) Klebsiella was inoculated into the seed medium, and cultured on a shaking table at 37° C. and 200 rpm for 24 hours;

[0044] (2) Insert the bacterium culture liquid of step (1) into the fermentation medium according to the inoculum size (v / v) of 10%, the pH value of the fermentation liquid is 7.0, the initial glycerol concentration of the fermentation liquid is 40g / L, and the , 200rpm under the shaking table for 24h, to get 1,3-propanediol fermentation broth, at this time the OD of the fermentation broth 600nm reached 8.47, the concentration of 1,3-propanediol reached 68.3g / L, the concentration of remaining glycerin was 15.3g / L, and the mass conversion rate of glycerol to 1,3-propanediol was 43.7%.

[0045] Pretreatment of 1,3-propanediol fermentation broth: the 1,3-propanediol fermentation broth obtained in the above 2 contains bacteria, which can be pretreated by flocculation, filtration, microfiltrat...

Embodiment 1

[0065] Embodiment 1 The phase formation law of glucose and three kinds of organic solvents

[0066]Use the turbidity method to draw the phase diagram of the two-phase system, prepare a nearly saturated aqueous glucose solution, add an organic solvent to it and mix evenly, record the mass fraction of the organic solvent and sugar when it happens to be turbid, and add a certain amount of distilled water to the system. Repeat the above steps after clarification, and draw the phase diagram of the aqueous two-phase system with the mass fraction of glucose as the horizontal axis and the mass fraction of the organic solvent as the vertical axis after the operation is completed.

[0067] The phase diagrams of the systems composed of different concentrations of glucose and acetone, n-propanol, and isopropanol are shown in figure 1 show. figure 1 The results show that the system composed of glucose and n-propanol has a relatively wide phase-forming range, and the phase-forming ability ...

Embodiment 2

[0068] Embodiment 2 The influence of different sugar / organic solvent systems on 1,3-diol separation effect

[0069] The simulated solution of 1,3-propanediol fermentation broth was prepared with pure 1,3-propanediol and distilled water. Wherein, the concentration of 1,3-propanediol in the 1,3-propanediol simulated liquid is 60g / L. Glucose was added to the 1,3-propanediol simulated liquid until it was completely dissolved to obtain a sugar-containing mixed liquid, and an organic solvent was added to the sugar-containing mixed liquid to form an extraction system. In the extraction system, the sugar, organic solvent, and 1,3-propanediol simulated liquid The mass percentages were 22%, 55%, and 23% (ω / ω), and the system was mixed uniformly, left to stand at room temperature to obtain a two-phase aqueous system, and samples were taken to determine the content of 1,3-propanediol in the upper phase and the lower phase. Concentration, calculate the distribution coefficient (K) and rec...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
recovery rateaaaaaaaaaa
Login to view more

Abstract

The invention provides a method of extracting and fermenting and separating 1,3-propylene glycol in fermentation liquor by coupling. The method comprises the following steps: dissolving sugar or / and inorganic salt in 1,3-propylene glycol fermentation liquor to obtain a sugar-containing mixed liquid; adding an organic solvent into the sugar-containing mixed liquid, oscillating and mixing, standing and centrifugalizing to split-phase at 15-40 DEG C to obtain an extracting system with the upper organic phase and the lower aqueous phase; recovering the organic solvent in the organic phase to obtain a 1,3-propylene glycol crude product; removing the organic solvent in the aqueous phase, adding water to dilute till the sugar concentration is 50-200g / L, inoculating thallus for fermenting the 1,3-propylene glycol. The method provided by the invention can be used for improving the utilization ratio of raw materials and efficiency of extraction and separation, reducing discharge of wastewater and lowering the extraction cost, so that fermentation and extraction of 1,3-propylene glycol are organically combined to couple fermentation and separation. The method is a novel separating method with industrial application prospect.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, relates to the separation technology of microbial fermentation liquid, in particular to a method for separating 1,3-propanediol in microbial fermentation liquid by using extraction technology coupled fermentation. Background technique [0002] 1,3-Propanediol is an important chemical raw material with a wide range of uses, and can be used as a solvent, antifreeze and protective agent, and as a monomer for the synthesis of polyester and polyurethane. Polyester (PTT) synthesized with 1,3-propanediol (1,3-PD) has better characteristics than polyester (PET) with ethylene glycol as monomer, such as: anti-ultraviolet, low static , the surface tension becomes smaller, and the anti-fouling ability is strong; the resilience is good, the resilience of PTT is twice that of PET, and it can quickly recover its original shape under external force; the dyeability is good, and the dyeing temperature is lo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/18C07C31/20C07C29/76
CPCY02P20/582
Inventor 修志龙绳傲楠高兴孙亚琴牟英
Owner DALIAN UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap