A Novel Seawater Fermentation Strain Producing Biosurfactant

Abstract

Traditional fermentation generally uses fresh water resources. The invention discloses a novel seawater fermentation type strain producing biosurfactant, which can well utilize natural seawater as fermentation water and waste edible oil as single carbon source for fermentation to produce biosurfactant rhamnolipid. By setting up seawater fermentation enterprises in areas with lack of coastal freshwater resources and poor water quality, the dependence of traditional fermentation industry on freshwater resources can be solved. The strain of the invention is isolated and purified through seawater enrichment culture, primary screening of high-salt blood plate, re-screening of synthetic medium and seawater shake flask fermentation verification, and through physiological and biochemical experiments and molecular identification analysis, it is determined that the seawater fermentation type strain is verdigris verdigris Pseudomonas aeruginosa has been deposited in the General Microbiology Center of the China Microorganism Culture Collection Management Committee on April 25, 2014, and the deposit number is: CGMCC No.9091. The strain of the invention utilizes the advantages of seawater fermentation, and the obtained crude product rhamnolipid can be used for regional market promotion, and will play a role in the fields of agriculture, petrochemical industry, environmental management, soil remediation, cosmetics industry, pharmaceutical industry and food industry. potential application prospects.

Description

technical field [0001] The invention belongs to the field of fermentation engineering, and in particular relates to a seawater fermentation type bacterial strain using natural seawater as fermentation water and waste edible oil as a single carbon source to ferment and produce biosurfactant rhamnolipid. Background technique [0002] Most of the surfactants currently on the market are mainly derived from petroleum-based chemical products. The production process requires a large amount of petroleum products and is easy to cause environmental pollution. However, the acquisition of biosurfactants does not have such problems. Biosurfactant rhamnolipid is a new type of anionic biosurfactant obtained from the secondary metabolism of microbial fermentation. It has the characteristics of biodegradability, low toxicity and environmental friendliness. And because rhamnolipid has good emulsification, wettability, and foaming properties, it has potential application prospects in agricultu...

AI Technical Summary

Problems solved by technology

Most of the traditional fermentation water is fresh water, and the cost of seawater desalination is relatively high at present. Therefore, it is of special significance to use new seawater fermentation strains to produce biosurfactant rhamnolipids in coastal tidal areas where fresh water is scarce and the water quality is poor.

[0004] The cost of fermented carbon source raw materials is also an important part of the production cost of fermentation engineering, but waste edible oils such as gutter oil and frying oil have not been well utilized at present Instead, it becomes a serious environmental pollutant or is unreasonably recycled and returned to the dining table, endangering people's health

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