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Method for preparing electrochemical sensor based on carbon nano tube and vinyl ferrocene modified carbon paste electrode

A vinyl ferrocene, carbon nanotube technology, applied in the direction of material electrochemical variables, can solve the problems of poor electron transfer efficiency, use of toxic organic solvents, high overpotential, achieve effective electrocatalytic activity, reduce anode overpotential Effect

Inactive Publication Date: 2014-10-15
WUXI BAILING SENSING TECH
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AI Technical Summary

Problems solved by technology

Tryptophan is a regular part of most protein-based diets, but high doses can cause side effects such as tiredness, nausea, dizziness, and loss of appetite
The methods for detecting tryptophan include fluorescence method, capillary electrophoresis, high performance liquid chromatography and spectrometry. These methods have many disadvantages: such as the need for complex detection equipment, professional operation skills, long analysis time, complicated sample pretreatment , the use of toxic organic solvents, etc.
[0004] Electrochemical technology is fast, simple, sensitive and cheap, and has been widely used. However, on the surface of traditional electrodes, tryptophan has poor electron transfer efficiency and high overpotential during oxidation.

Method used

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Embodiment Construction

[0012] In order to deepen the understanding of the present invention, the present invention will be further described below in conjunction with implementation.

[0013] A preparation method of an electrochemical sensor based on carbon nanotubes and vinyl ferrocene modified carbon paste electrodes, the steps are as follows:

[0014] Electrode preparation: 0.01 g of vinylferrocene, 0.89 g of graphite powder and 0.1 g of carbon nanotubes were mixed in a mortar, 0.9 mL of paraffin oil was added to the mixture and mixed for 40 min until a uniform paste was formed. The paste was squeezed into the end of a glass tube with a diameter of 0.6 mm and a length of 3 cm, and a copper wire was inserted into the carbon paste as a wire.

[0015] Electrochemical measurement procedure: Electrochemical measurement is carried out on an electrochemical workstation, and a traditional three-electrode electrolytic cell is used for measurement. Ag / AgCl / KCl (1.5mol / L), platinum wire, carbon nanotubes a...

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Abstract

The invention relates to a method for preparing an electrochemical sensor based on a carbon nano tube and vinyl ferrocene modified carbon paste electrode. Vinyl ferrocene, powdered graphite and a carbon nano tube are adopted to prepare the carbon nano tube and vinyl ferrocene modified carbon paste electrode so as to form the electrochemical sensor. The prepared electrochemical sensor can reduce the overpotenial of the anode, shows effective electrocatalysis activity, and therefore, can be used for quantitative detection of adrenalin with existence of tryptophane.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical sensor, in particular to a preparation method of an electrochemical sensor for simultaneously detecting adrenaline and tryptophan based on carbon nanotubes and vinyl ferrocene modified carbon paste electrodes. Background technique [0002] Epinephrine (1-(3,4-dihydroxyphenyl)-2-methoxyaminoethanol) is a hormone secreted by the adrenal medulla and an important catecholamine neuron in the mammalian central nervous system transferrin. The existence of adrenaline in the human body can affect blood pressure regulation, heart rate, lipolysis, immune system and glucose metabolism, and one of the main manifestations of patients with Parkinson's disease is the low level of adrenaline. Therefore, it is harmful to the human body Quantitative detection of adrenaline in plasma and urine is very important for scientific research and human health. [0003] Tryptophan is an essential amino acid in t...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/26
Inventor 孙京华潘正海
Owner WUXI BAILING SENSING TECH
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