Micro-fluidic chip-based pesticide residue detection method
A pesticide residue detection, microfluidic chip technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of high technical level requirements of analysts, cumbersome pretreatment process, uncomfortable rapid detection, etc., and achieve high analysis efficiency. , The effect of low resistance voltage drop and low consumption
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Embodiment 1
[0033] Example 1 Preparation steps of an immunosensor based on a microfluidic chip:
[0034] (1) Cleaning of the microfluidic chip: first pass through 0.1 M sodium hydroxide, stand still for 1 hour, pass through double distilled water for 30 minutes, rinse thoroughly; then pass through 0.1 M hydrogen chloride, stand still for 1 hour, pass through double distilled water After 30 min, wash it clean; the flow rate in the whole test cleaning is 10 μL / min; finally, after washing with ultrapure water, scan the impedance spectrum, and compare with the result of the bare electrode last time, the two are basically coincident, indicating that Clean it, otherwise it needs to be cleaned again. If necessary, the surface state of the gold-intercalated microarray electrodes in microfluidics can be observed through a microscope. (The cleaning process was compared with ethanol, acetone and other methods to compare the cleaning effect, and an appropriate cleaning method was selected);
[0035...
Embodiment 2
[0037]Example 2 Linear relationship of microfluidic chip
[0038] Configuration 1-1.0×10 4 ng / mL chlorpyrifos standard solution, the microfluidic chip immunosensor prepared above was injected into different concentrations of chlorpyrifos standard solution at a flow rate of 10 μL / min, incubated at room temperature for 30 min, and the current change before and after immunization was detected to obtain its Working curve. Such as figure 1 , the curves respectively represent the impedance values corresponding to different chlorpyrifos pesticide concentrations, and the impedance values increase gradually with the increase of the concentration. In this test, the logarithm of the concentration of chlorpyrifos is used as the abscissa, and the impedance change value is used as the ordinate to establish a relationship curve, which can be obtained at 1-1.0×10 4 ng / mL range linear correlation, the linear regression equations are: y=65.580 lgC (ng / mL) + 9.8400 (R 2 =0.9868)( fig...
Embodiment 3
[0039] Example 3 Determination of spiked recovery of fruit and vegetable samples using microfluidic chip immunosensor
[0040] Select fresh leeks, lettuce, and cabbage as samples, wash and dry them, chop them into 3×3 mm pellets, weigh 1.0 g each accurately, put them into 3 weighing bottles, and then add the same volume of 10 ng / mL , 100 ng / mL, and 1000 ng / mL chlorpyrifos standard solutions, then add 5 mL of buffer solution to three weighing bottles, ultrasonically extract for 30 min, and centrifuge the extracted solution to remove vegetable residues and large particle interference things. The supernatant was taken for membrane filtration, and 2.5 mL was taken in three test tubes after membrane filtration. The impedance value measurement before and after contacting the sample is carried out by using the microfluidic chip immunosensor of the present invention. After the measurement, the actual detection value is obtained according to the obtained relationship curve between t...
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