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Hyaluronic acid modified surface plasma resonance spectrometer chip and preparation method thereof

A technology of surface plasmon and hyaluronic acid, applied in the direction of instruments, scientific instruments, material inspection products, etc., can solve the problem of high price of dextran series chips, and achieve the effect of high solid loading and high biocompatibility

Inactive Publication Date: 2014-11-19
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chip only has good anti-adsorption performance for a single protein solution, and basically cannot meet the requirements for more complex systems, such as soybean milk and milk.
On the other hand, due to the monopoly of foreign companies, the dextran series chips are expensive

Method used

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  • Hyaluronic acid modified surface plasma resonance spectrometer chip and preparation method thereof
  • Hyaluronic acid modified surface plasma resonance spectrometer chip and preparation method thereof
  • Hyaluronic acid modified surface plasma resonance spectrometer chip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A 2nm-thick chromium layer was first coated on the BK7 glass substrate by vacuum sputtering, and then a 48nm-thick gold film or gold-silver alloy (mixed ratio) was coated to obtain a bare gold chip. Immerse the bare gold chip in a mixed solution of concentrated sulfuric acid and hydrogen peroxide (volume ratio 1:0.4) for 3 hours at room temperature. Then take it out, wash it with deionized water and dry it with nitrogen gas. The dried bare gold chip was immersed in 5 mM 11-mercapto-1-undecanol solution (prepared in ethanol) for 12 hours at room temperature. After cleaning with ethanol and deionized water and drying with nitrogen, immerse in 0.6M epichlorohydrin solution (prepared with diethylene glycol dimethyl ether), and soak for 4 hours at room temperature. After washing with deionized water and blowing dry with nitrogen, immerse in a hyaluronic acid solution (prepared with 0.1M sodium hydroxide solution) with a concentration of 1 mg / mL and a molecular weight of 10 ...

Embodiment 2

[0035]A 2nm-thick chromium layer was first coated on the BK7 glass substrate by vacuum sputtering, and then a 48nm-thick gold film or gold-silver alloy (mixed ratio) was coated to obtain a bare gold chip. Immerse the bare gold chip in a mixed solution of concentrated sulfuric acid and hydrogen peroxide (volume ratio 1:0.6) for 3 hours at room temperature. Then take it out, wash it with deionized water and dry it with nitrogen gas. The dried bare gold chip was immersed in 5 mM 11-mercapto-1-undecanol solution (prepared in ethanol) for 12 hours at room temperature. After cleaning with ethanol and deionized water and drying with nitrogen, immerse in 0.6M epichlorohydrin solution (prepared with diethylene glycol dimethyl ether), and soak for 4 hours at room temperature. After washing with deionized water and blowing dry with nitrogen, immerse in a hyaluronic acid solution (prepared with 0.1M sodium hydroxide solution) with a concentration of 3 mg / mL and a molecular weight of 1000...

Embodiment 3

[0037] A 2nm-thick chromium layer was first coated on the BK7 glass substrate by vacuum sputtering, and then a 48nm-thick gold film or gold-silver alloy (mixed ratio) was coated to obtain a bare gold chip. Immerse the bare gold chip in a mixed solution of concentrated sulfuric acid and hydrogen peroxide (volume ratio 1:1) for 3 hours at room temperature. Then take it out, wash it with deionized water and dry it with nitrogen gas. The dried bare gold chip was immersed in 5 mM 11-mercapto-1-undecanol solution (prepared in ethanol) for 12 hours at room temperature. After cleaning with ethanol and deionized water and drying with nitrogen, immerse in 0.6M epichlorohydrin solution (prepared with diethylene glycol dimethyl ether), and soak for 4 hours at room temperature. After washing with deionized water and blowing dry with nitrogen, immerse in hyaluronic acid solution (prepared with 0.1M sodium hydroxide solution) with a concentration of 10 mg / mL and a molecular weight of 2000 k...

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Abstract

The invention relates to a hyaluronic acid modified surface plasma resonance spectrometer chip and a preparation method thereof. The structure of the hyaluronic acid modified chip is orderly composed of a glass layer, a chromium layer, a gold film layer, a mercapto-alcohol monomolecular layer and a hyaluronic acid layer. The surface of the gold film is modified by use of mercaptoundecanol and then a self-assembly single layer can be formed; next, the self-assembly single layer is epoxidized by use of epoxy chloropropane and then immersed into a hyaluronic acid solution, covalently modified with a layer of hyaluronic acid and further subjected to carboxylation by use of bromoacetic acid, and finally, the hyaluronic acid-based surface plasma resonance spectrometer chip can be obtained. The chip has excellent antifouling property in a single protein, a natural complex sample and 10% blood serum, and has high immobilization capacity and excellent compatibility for biomacromolecular antibodies, and high-specificity sensitive quick detection on target molecules in a complex protein system is realized. The chip has excellent antifouling property, and is low in raw material cost; the preparation method of the chip is easy to operate; the chip has excellent economic feasibility and practicability.

Description

technical field [0001] The invention relates to a hyaluronic acid-modified surface plasmon resonance chip and a preparation method thereof, belonging to the design and preparation method of an anti-pollution chip of a surface plasmon resonance spectrometer. Background technique [0002] Surface plasmon resonance (SPR) spectrometer is generally favored by large foreign instrument companies because of its advantages of simplicity, speed, and high sensitivity, and has begun large-scale research and development. It has developed into a qualitative or quantitative measurement of molecular interactions. It has been widely used in biotechnology, medical testing, environmental science, food safety, drug screening, material analysis and many other fields. [0003] The SPR chip (such as the CM5 series of American General Company, which is widely used at present, is connected with a layer of dextran on the bare gold film) is the core component of the surface plasmon resonance spectrome...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N21/554
Inventor 苏荣欣黄仁亮刘霞齐崴何志敏
Owner TIANJIN UNIV
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