Hyaluronic acid modified surface plasma resonance spectrometer chip and preparation method thereof
A technology of surface plasmon and hyaluronic acid, applied in the direction of instruments, scientific instruments, material inspection products, etc., can solve the problem of high price of dextran series chips, and achieve the effect of high solid loading and high biocompatibility
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[0032] Example 1
[0033] By vacuum sputtering, a 2nm-thick chromium layer was first coated on the BK7 glass substrate, and then a 48nm-thick gold film or gold-silver alloy (any mixing ratio) was coated to obtain a bare gold chip. Immerse the bare gold chip in a mixed solution of concentrated sulfuric acid and hydrogen peroxide (volume ratio 1:0.4) for 3 hours at room temperature. It was then removed, rinsed with deionized water and dried with nitrogen. Immerse the dried bare gold chip in a 5mM 11-mercapto-1-undecanol solution (prepared with ethanol) for 12 hours at room temperature. After washing with ethanol and deionized water and drying with nitrogen, it was immersed in 0.6M epichlorohydrin solution (prepared with diethylene glycol dimethyl ether) for 4 hours at room temperature. After washing with deionized water and drying with nitrogen, immerse in hyaluronic acid solution with a concentration of 1 mg / mL and a molecular weight of 10 kDa (prepared with 0.1 M sodium hydr...
Example Embodiment
[0034] Example 2
[0035]By vacuum sputtering, a 2nm-thick chromium layer was first coated on the BK7 glass substrate, and then a 48nm-thick gold film or gold-silver alloy (any mixing ratio) was coated to obtain a bare gold chip. Immerse the bare gold chip in a mixed solution of concentrated sulfuric acid and hydrogen peroxide (volume ratio 1:0.6) for 3 hours at room temperature. It was then removed, rinsed with deionized water and dried with nitrogen. Immerse the dried bare gold chip in a 5mM 11-mercapto-1-undecanol solution (prepared with ethanol) for 12 hours at room temperature. After washing with ethanol and deionized water and drying with nitrogen, it was immersed in 0.6M epichlorohydrin solution (prepared with diethylene glycol dimethyl ether) for 4 hours at room temperature. After washing with deionized water and drying with nitrogen, immerse in hyaluronic acid solution with a concentration of 3mg / mL and a molecular weight of 1000kDa (prepared with 0.1M sodium hydrox...
Example Embodiment
[0036] Example 3
[0037] By vacuum sputtering, a 2nm-thick chromium layer was first coated on the BK7 glass substrate, and then a 48nm-thick gold film or gold-silver alloy (any mixing ratio) was coated to obtain a bare gold chip. Immerse the bare gold chip in a mixed solution of concentrated sulfuric acid and hydrogen peroxide (volume ratio 1:1) for 3 hours at room temperature. It was then removed, rinsed with deionized water and dried with nitrogen. Immerse the dried bare gold chip in a 5mM 11-mercapto-1-undecanol solution (prepared with ethanol) for 12 hours at room temperature. After washing with ethanol and deionized water and drying with nitrogen, it was immersed in 0.6M epichlorohydrin solution (prepared with diethylene glycol dimethyl ether) for 4 hours at room temperature. After washing with deionized water and drying with nitrogen, it was immersed in a hyaluronic acid solution with a concentration of 10 mg / mL and a molecular weight of 2000 kDa (prepared with a 0.1 ...
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