Polysaccharide extraction method based on epoxy magnetic microspheres

A technology for extracting magnetic microspheres and polysaccharides, which is applied in the field of separation of biologically active substances, can solve the problems of extracting polysaccharides from magnetic microspheres and other problems, and achieves the effect of simple steps and time saving.

Inactive Publication Date: 2014-12-03
TAISHAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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Examples

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Effect test

Embodiment 1

[0016] Example 1: Preparation of Magnetic Microspheres and Its Use in the Extraction of Poria Cocos Polysaccharide

[0017] Take 1 g of sodium citrate-modified iron ferric oxide nanoparticles, add 2 mL of water to ultrasonically disperse, mix with 10 mL of No. 3 water glass, and call it the water phase. Take 0.5g of OP emulsifier and 3g of Span-80, dissolve it in 20 mL of dichloromethane, and call it the oil phase. Mix the water phase with the oil phase and treat it with an ultrasonic cell breaker for 5 minutes to obtain a water-in-oil emulsion; take 75 mL of 2 mol / L ammonium sulfate aqueous solution and place it on a magnetic stirrer at a speed of 800 rpm, slowly add the emulsion and continue stirring For 1 h, the solid matter was sucked out with a magnet, and washed twice with distilled water to obtain magnetic microspheres with an average particle diameter of 5 μm, and the spherical shape was relatively round. Disperse the microspheres into 100 times the volume of water an...

Embodiment 2

[0021] Example 2: Effects of Magnetic Microspheres with Different Surface Properties on the Extraction Purity of Poria Cocos Polysaccharide

[0022] Take 1 g of ferric oxide nanoparticles modified by sodium citrate, add 1 mL of water to ultrasonically disperse, mix with 5 mL of No. 3 water glass, and call it the water phase. Take 0.3g of OP emulsifier and 2g of Span-80, dissolve it in 20 mL of chloroform, and call it the oil phase. Mix the water phase with the oil phase and treat it with a high-speed shear emulsifier for 5 minutes to obtain a water-in-oil emulsion; take 75 mL of a saturated sodium bicarbonate aqueous solution and place it on a magnetic particle stirrer at a speed of 800 rpm, slowly add the emulsion and continue stirring For 1 h, the solid matter was sucked out with a magnet, and washed twice with distilled water to obtain magnetic microspheres.

[0023] Dry the above-mentioned magnetic microspheres in an oven at 100°C for 5 hours, take three portions of magne...

Embodiment 3

[0025] Example 3: Extraction of Danshen polysaccharides assisted by magnetic microspheres

[0026] Take 1g of ferric oxide nanoparticles modified by sodium citrate, add 1mL of water to ultrasonically disperse, mix with 10mL of No. 3 water glass, and call it the water phase. Take 0.5g of OP emulsifier and 3g of Span-80, dissolve it in 20 mL of petroleum ether, and call it the oil phase. Mix the water phase with the oil phase and treat it with an ultrasonic cell breaker for 5 minutes to obtain a water-in-oil emulsion; take 75 mL of 2 mol / L ammonium chloride aqueous solution and place it on a magnetic particle stirrer at a speed of 800 rpm, and slowly add the emulsion. Stirring was continued for 1 h, the solid matter was sucked out with a magnet, and washed twice with distilled water to obtain magnetic microspheres.

[0027] Dry the above magnetic microspheres in an oven at 100°C for 5 hours, take 1g of magnetic microspheres, ultrasonically disperse them in 50mL of ethanol with ...

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Abstract

The invention relates to the technical field of separation of biological active substances and provides a preparation method of magnetic microspheres and a method for extracting water-soluble polysaccharides from a waterborne matrix by virtue of the microspheres. The method comprises the following steps: firstly, mixing citric acid modified magnetic nanoparticles with water glass, ultrasonically dispersing in emulsifier-containing dichloromethane to form water-in-oil emulsion, pouring the emulsion into a buffer solution, and curing the water glass to form magnetic microspheres of a SiO2 matrix; secondly, performing surface modification by using epoxy silane, adding the microspheres into polysaccharide crude extract liquid, adding ethanol with weight being 1-2 times that of the polysaccharide crude extract liquid, enabling polysaccharides to form flocculated precipitates for wrapping the magnetic microspheres, and performing solid-liquid separation by using a magnet. According to the method, the polysaccharide recovery rate is equivalent to that of a conventional centrifugal method but the protein impurity content is remarkably reduced, the process is simplified, the time consumption is only 1/10 that of the conventional method, and the magnetic microspheres can be repeatedly utilized; the method has wide application prospects in the field of biological separation, especially pretreatment of extraction, separation and sample analysis of animals, plants and fungal polysaccharides.

Description

technical field [0001] The invention relates to the technical field of separation of bioactive substances, in particular to a method for extracting polysaccharides from an aqueous matrix containing polysaccharides by using surface-modified magnetic microspheres. Background technique [0002] Polysaccharides are polymers linked by monosaccharides, which are widely found in animals, plants, fungi and microorganisms. Polysaccharides have significant medicinal or health value in immune regulation, anti-tumor, hypoglycemic, hypolipidemic, anti-aging, liver protection, etc., and have almost no toxic side effects on the human body. The extensive attention in the field has also made the research on polysaccharide extraction and purification methods more important. [0003] The extraction method of polysaccharides traditionally adopts water extraction and alcohol precipitation method, which is mainly based on the characteristic that polysaccharides are soluble in water but not solub...

Claims

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Application Information

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IPC IPC(8): C08B37/00B01J13/02
Inventor 毕研平郝吉福王建筑
Owner TAISHAN MEDICAL UNIV
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