Method for preparing astragalus Sanxian soup flexible nano-liposome

A technology of nano-liposomes and astragalus, which is applied in the field of medicine, can solve the problems of difficult research, poor stability, and low drug loading of traditional Chinese medicine liposomes, and achieve good targeting, high encapsulation efficiency, and uniform particle size. Effect

Inactive Publication Date: 2014-12-10
GUANGDONG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of liposomes in the field of traditional Chinese medicine has also made gratifying achievements. Its characteristics of improving curative effect, reducing toxicity, targeting, sustained release, and cell affinity have been recognized, but there are still some problems in the research of liposomes in traditional Chinese medicine. : The research of liposomes of traditional Chinese medicine is mainly based on the research of liposomes of single active drug components, which is essentially the same as the research of liposomes of western medicines. Only liposomes with multiple components of traditional Chinese medicines can reflect the characteristics of Chinese medicines , also in line with the characteristics of traditional Chinese medicine
Some traditional Chinese medicine liposomes have low drug loading and poor stability, which needs to be further solved
Compared with western medicine, the complex and diverse chemical components of traditional Chinese medicine make the study of liposomes of traditional Chinese medicine much more difficult

Method used

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  • Method for preparing astragalus Sanxian soup flexible nano-liposome
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  • Method for preparing astragalus Sanxian soup flexible nano-liposome

Examples

Experimental program
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Effect test

Embodiment 1

[0036] (1) Weigh 80 mg of lecithin, 5 mg of cholesterol, 3 mg of astragaloside IV, 1 mg of icariin, and 110 mg of notoginseng saponin R, then put them into a container, add a mixed solvent of methanol and chloroform to fully dissolve it, and the volume of the mixed solvent is 0.5 ml;

[0037] (2) Then the dissolved raw material is placed on a rotary evaporator, and in a constant temperature water bath at 30°C, the organic solvent is removed by rotary evaporation under reduced pressure, and a uniform lipid film is formed on the bottle wall, and the rotary evaporation is continued for 10 minutes to ensure that the organic solvent After the removal is complete, after the vacuum rotary evaporation is completed, vacuum dry overnight;

[0038] (3) prepare the sodium cholate PBS solution of 5ml, the add-on of sodium cholate in the sodium cholate PBS solution is 2.5mg, then add ethylenediaminetetramethylene phosphoric acid, ethylenediaminetetramethylidene in the sodium cholate PBS sol...

Embodiment 2

[0042] (1) Weigh 120 mg of lecithin, 15 mg of cholesterol, 10 mg of astragaloside IV, 5 mg of icariin, and 115 mg of notoginseng saponin R, and then put them into a container, add a mixed solvent of methanol and chloroform to fully dissolve it, and the volume of the mixed solvent and cholesterol The weight ratio is 30ml;

[0043] (2) Then the dissolved raw material is placed on a rotary evaporator, and in a constant temperature water bath at 50°C, the organic solvent is removed by rotary evaporation under reduced pressure, and a uniform lipid film is formed on the bottle wall, and the rotary evaporation is continued for 20 minutes to ensure that the organic solvent After the removal is complete, after the vacuum rotary evaporation is completed, vacuum dry overnight;

[0044] (3) prepare the sodium cholate PBS solution of 15ml, the add-on of sodium cholate in the sodium cholate PBS solution is 30mg, then add ethylenediaminetetramethylene phosphoric acid, ethylenediaminetetramet...

Embodiment 3

[0048] (1) Weigh 100mg of lecithin, 10mg of cholesterol, 6mg of astragaloside IV, 3mg of icariin, and 113mg of notoginsenoside R113mg, and then put them into a container, add a mixed solvent of methanol and chloroform to fully dissolve it, and the volume of the mixed solvent and cholesterol The weight ratio is 10ml;

[0049] (2) Then the dissolved raw material is placed on a rotary evaporator, and in a constant temperature water bath at 40°C, the organic solvent is removed by rotary evaporation under reduced pressure, and a uniform lipid film is formed on the bottle wall, and the rotary evaporation is continued for 15 minutes to ensure that the organic solvent After the removal is complete, after the vacuum rotary evaporation is completed, vacuum dry overnight;

[0050] (3) the sodium cholate PBS solution of preparation 10ml, the add-on of sodium cholate in the sodium cholate PBS solution is 10mg, then add ethylenediaminetetramethylene phosphoric acid, ethylenediaminetetrameth...

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Abstract

The invention discloses a method for preparing astragalus Sanxian soup flexible nano-liposome. The preparation method comprises the following steps: (1) weighing lecithin, cholesterol, astragaloside, icariin and notoginsenoside R1 according to a ratio, adding the raw materials into a container, and adding a methanol-chloroform mixed solvent, so that the raw materials are fully dissolved; (2) arranging the dissolved raw materials on a rotary evaporator, performing reduced pressure spin evaporation to remove an organic solvent in a constant temperature water bath, and performing vacuum drying overnight; (3) preparing a sodium cholate PBS solution, adding ethylene diamine tetramethylidene phosphoric acid into the sodium cholate PBS solution, adding the mixed solution into the dried raw materials, and performing normal pressure spin evaporation in the constant temperature water bath, thereby preparing primary suspension of the liposome; and (4) ultrasonically oscillating the primary suspension of the liposome, and finally sequentially squeezing the primary suspension to pass through microfiltration membranes with the pore diameters of 0.80mu m, 0.45mu m and 0.22mu m to obtain the flexible nano-liposome. The liposome disclosed by the invention has the advantages of uniform particle size, high encapsulation efficiency and high targeting property.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for preparing flexible nano-liposomes of Huangqi Sanxian Decoction. Background technique [0002] Osteoporosis is a systemic bone disease characterized by loss of bone mass and destruction of bone tissue microarchitecture due to uncoupling of bone resorption and bone formation, resulting in increased bone fragility and susceptibility to fracture. The World Health Organization defines osteoporosis as a decrease in bone mineral density (BMD) or bone mineral content (BMC) of more than 2.5 standard deviations from the mean of normal adult BMD. Osteoporosis is divided into primary osteoporosis and secondary osteoporosis according to the etiology. Clinical manifestations include pain, decreased bone density, shortened body length, kyphosis, decreased respiratory function, and fractures. With the prolongation of life expectancy and the increase of elderly population, osteopo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K31/7048A61P19/10A61K31/704
Inventor 周志昆朱建红郑丽华郭翀翀
Owner GUANGDONG MEDICAL UNIV
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