Degradable endocranium repair stent compounded by human amniotic membrane and bull dorsal aponeurosis and preparation method of repair stent

Abstract

The invention provides a preparation method of a degradable endocranium repair stent compounded by a human amniotic membrane and bull dorsal aponeurosis. The preparation method comprises the following steps: (1) preparing an amniotic membrane; (2) preparing bull dorsal aponeurosis pieces; and (3) performing non-toxic crosslinking for amniotic membrane and bull dorsal aponeurosis pieces. The endocranium repair stent compounded by a human amniotic membrane and bull dorsal aponeurosis comprises the prepared amniotic membrane and the bull dorsal aponeurosis pieces; the prepared amniotic membrane and the bull dorsal aponeurosis pieces are subjected to non-toxic crosslinking to obtain the endocranium repair stent compounded by a human amniotic membrane and bull dorsal aponeurosis. According to the preparation method, the amniotic membrane processing method meets national standard, and the biological activity function of the amniotic membrane can be remained; the preparation of the bull dorsal aponeurosis pieces is different from the preparation of other collagen membranes, namely, the cell, nucleic acid and other immunogenicity substances can be removed without damaging the structure of the natural bull dorsal aponeurosis collagen, and the mechanical property is remained; in addition, the biosecurity is improved; and the two natural collagen membranes are subjected to non-toxic crosslinking and freezing and drying, and all functions meet the clinical requirements of repair of endocranium.

Description

technical field [0001] The invention relates to a dura mater repair bracket, in particular to a dura mater repair bracket capable of degradable human amniotic membrane and bovine dorsal tendon compounding and a preparation method thereof. Background technique [0002] The dura mater is an important barrier to protect brain tissue. It is a thick and tough double-layer membrane. The outer layer of the dura mater is the periosteum on the inner surface of the skull, which is loosely attached to the calvaria, especially in the occipital and temporal parts, and is called the periosteum layer; the inner layer is thicker and tougher than the outer layer, and is connected with the dura mater in the occipital bone The large hole is continuous and is called the meningeal layer. Trauma, tumor erosion, and surgery can cause dural defects, which must be repaired to prevent complications such as cerebrospinal fluid leakage, intracranial infection, and epilepsy caused by cerebral cortical ...

AI Technical Summary

Problems solved by technology

[0003] At present, there are two clinical treatment methods for dura mater repair: 1. Suture type, using artificial dura mater repair materials (such as "meningeal guard", which is processed from bovine pericardium) to directly suture the defect, and suture it with its own meninges. This method is suitable for small leaks. For patients with acute hypertensive cerebral herniation and encephalocele after decompression, the suturing operation is difficult and the brain tissue is easily damaged during suturing.

2. Adhesive type, using artificial dura mater repair materials (such as collagen sponge) to directly attach to the defect, clinically, the adhesive is not strict, cerebrospinal fluid leaks, and epimeningeal effusion is easy to form

[0012] 1. The combination of small intestinal submucosa (about 0.08mm) and human amniotic membrane (about 0.05mm) is used. The thickness is quite different from that of dura mater, and the suture performance and mechanical properties are poor and cannot meet the requirements

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