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Endogenous Polychaeta euvecoides in a Artemisia annua and its application

A technology of Pseudomonas polychaetes and Artemisia spp., which is applied in the field of microorganisms and can solve problems such as the types of unsafe microorganism-derived medicines.

Active Publication Date: 2016-08-17
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of endophytic fungus isolated and screened from the medicinal plant Artemisia annua ---Pestalotiopsis uvicola in view of the shortage of unsafe chemical drugs and fewer types of microbial source drugs

Method used

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  • Endogenous Polychaeta euvecoides in a Artemisia annua and its application
  • Endogenous Polychaeta euvecoides in a Artemisia annua and its application
  • Endogenous Polychaeta euvecoides in a Artemisia annua and its application

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Embodiment 1

[0038] Embodiment 1: (Isolation and screening of Yuweike Discochaete GMH31)

[0039] In August 2011, the present invention collected the healthy leaves of Artemisia annua, a medicinal plant, in the suburbs of Guiyang City, Guizhou Province. After surface disinfection, separation of endophytic fungi, cultivation, fermentation, and activity determination, the active strains of pathogenic bacteria inhibiting plant fungal diseases and Screening of tumor cell lines and other steps, from which Uvecosporum GMH31 was obtained and preserved.

[0040] The specific isolation and cultivation methods are: (1) preparation of Artemisia annua plant tissue: collect the leaf tissue of Artemisia annua, rinse with tap water, remove the superficial microorganisms on the surface of the sample, and then put it on a clean gauze and blow it to dry;

[0041] (2) Indicate disinfection and sterility testing: the dried tissue pieces are cut into appropriate sizes, rinsed with sterilized distilled water, a...

Embodiment 2

[0049] Embodiment 2: (preparation method of Yuwei can be Pseudomonas GMH31 fermentation metabolites)

[0050] Follow these steps:

[0051] (1) Activation culture of strains: move the separated and preserved strains of P. GMH31 from Pseudomonas GMH31 to the potato dextrose agar PDA solid plate, and cultivate them at 28±1°C until the hyphae cover the entire culture dish. With a diameter of 7mm puncher punching to obtain bacteria cake, used for inoculation;

[0052] (2) Liquid fermentation culture: the fermentation medium contains 10g of corn flour, 20g of mannitol, 20g of glucose, 5g of yeast extract, 10g of peptone, and KH per 1000mL. 2 PO 4 0.5g, CaCO 3 15g, MgSO 4 ·7H 2 O 0.3g, 100mL of fermentation medium per 300mL Erlenmeyer bottle; after preparation, sterilize at 121°C for 20min, cool to 40°C, pick 1 bacterium cake with a diameter of 7mm under aseptic conditions, and stand at 28±1°C Place fermentation and culture for 30 days;

[0053] (3) Extraction of fermented liq...

Embodiment 3

[0056] Embodiment 3: (the inhibitory effect of Yuweike Discotrichum sp. GMH31 fermentation broth metabolites on kiwifruit brown rot fungus and Phytophthora capsici)

[0057] (1) Accurately weigh 0.1 g of the n-butanol fermentation broth extract of Polychaetospermum GMH31, add 10 mL of sterile water, fully dissolve with ultrasonic vibration, and finally prepare a mother liquor with a concentration of 10 g / L;

[0058] (2) Determination of antibacterial activity: Take the above mother liquor and dilute it into solutions with a concentration of 1000mg / L and 500mg / L respectively, put 1mL of each concentration solution in a sterile petri dish, and quickly mix it with 9mL of PDA medium cooled to 50°C Evenly (the final concentration of metabolites of Uvecodiscoides GMH31 is 100mg / L and 50mg / L), after cooling, put a 7mm diameter cake of kiwifruit brown rot and Phytophthora capsici respectively on each medium plane , the bacteria cake was connected to the center of the petri dish (the d...

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Abstract

The invention relates to the technical field of microorganisms. An artemisia japonica endophytic fungus is collected from living bodies of artemisia japonica from a suburb of Guiyang city in Guiyang province through endophytic fungus separation technology. The artemisia japonica endophytic fungus is named pestalotiopsis uvicola GMH 31 through authentication by microbial taxonomy. The strain is deposited in China center for type culture collection with a deposition date being June-30-2014 and an accession number being CCTCC No: M2014303. The invention has following most significant characteristics: (1) A fermentation liquid of the strain is an active metabolite which has inhibiting effects of sclerotinia sclerotiorum, phytophthora capsici and the like and is an important microbial resource; and (2) A fermentation mycelium of the strain can secret various natural active components, has medicinal effects of brad-spectrum tumor resistance and the like, and is an important microbial resource of a novel natural active drug.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the application of an endophytic fungus of the genus Pestalotiopsis uvicola GMH31 derived from the medicinal plant Artemisia annua and its metabolites in agriculture and medicine application. Background technique [0002] Fungal endophyte refers to the fungi in the qualified organs of various tissues of living and healthy plants at a certain stage or all stages of the life cycle. They form a close symbiotic relationship with host plants through interaction, which can promote plant growth and provide plants with the ability to adapt to stress. Some endophytic fungi also have medicinal value. Searching and discovering active compounds from plant endophytic fungi has become another research hotspot at home and abroad. For example, Chinese patent document CN201310194836.5 discloses an endophytic fungus (Metarrhizium) LD421 isolated from gentian, which can be used to prevent ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P1/02A01N63/04A01P3/00A61K36/06A61P35/00C12R1/645
Inventor 钱一鑫康冀川雷帮星
Owner GUIZHOU UNIV
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