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Method for producing isomalto-oligosaccharide by using high-concentration starch

A high-concentration isomaltooligosaccharide technology, which is applied in the field of producing high-purity isomaltooligosaccharide with high conversion rate, can solve the problems of low conversion rate and low yield of isomaltooligosaccharide, so as to increase yield and improve enzyme reaction The effect of speed and shortening the time of glycosylation

Inactive Publication Date: 2014-12-17
吉林省轻工业设计研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The invention provides a method for producing isomaltooligosaccharides from high-concentration starch to solve the problem of low yield of isomaltooligosaccharides due to the low conversion rate of α-glucosidase at present

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Liquefaction of high-concentration starch

[0025] Use 40℃ water and first-grade cornstarch to prepare 50% starch milk in the mixing tank. If the water temperature is too high, the starch is easy to gelatinize, and the water temperature is too low. Therefore, it is necessary to adjust the pulp with warm water, adjust the pH value to 5.6 with dilute hydrochloric acid (chemically pure) and sodium hydroxide (chemically pure) (this is the optimum pH value for Novozymes' high-temperature resistant amylase), according to each ton of starch (dry basis) ) Add 0.4 liters of high-temperature resistant α-amylase in the proportion of high-temperature resistant α-amylase, and then pump the starch milk from the mixing tank to the heat preservation liquefaction tank, heat it in the jacket with steam, and stir to the temperature while heating Stop stirring when it reaches 80°C, keep it warm for 30 minutes, then add 0.2 liters of heat-resistant amylase per ton of starch (dry basis) to ...

Embodiment 2

[0033] (1) High concentration starch liquefaction

[0034] Prepare 60% starch milk with 50℃ water and first-grade corn starch in the mixing tank, and adjust the pH to 5.6 with dilute hydrochloric acid (chemically pure) and sodium hydroxide (chemically pure) (this is Novozymes The optimum pH value of high temperature amylase), add 0.5 liters of high temperature resistant alpha amylase per ton of starch (dry basis), add high temperature resistant alpha amylase, and then pump the starch milk from the mixing tank to heat preservation and liquefaction In the tank, use steam to heat in the jacket, stir while heating until the temperature reaches 85℃, stop stirring, keep it for 40 minutes, and then add 0.3 liters of high temperature resistant alpha amylase per ton of starch (dry basis). High-temperature α-amylase, using jet liquefier for jet liquefaction, the temperature is controlled at 105°C, and then the flashing temperature is reduced to 90°C, the liquefied liquid is kept and stirre...

Embodiment 3

[0042] (1) High concentration starch liquefaction

[0043] Prepare 55% starch milk with 45℃ water and first-grade corn starch in the mixing tank, adjust the pH to 5.6 with dilute hydrochloric acid (chemically pure) and sodium hydroxide (chemically pure) (this is Novozymes The optimal pH value of high temperature amylase), add 0.45 liters of high temperature resistant alpha amylase per ton of starch (dry basis), add high temperature resistant alpha amylase, and then pump the starch milk from the mixing tank to heat preservation and liquefaction In the tank, use steam to heat in the jacket, stir while heating until the temperature reaches 83℃, stop stirring, keep it for 35 minutes, and then add 0.25 liters of high temperature resistant alpha amylase per ton of starch (dry basis). High-temperature α-amylase, using jet liquefier for jet liquefaction, the temperature is controlled at 105 ℃, and then the flashing temperature is reduced to 90 ℃, the liquefied liquid is kept in the lique...

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Abstract

The invention relates to a method for producing isomalto-oligosaccharide by using high-concentration starch and belongs to the field of production of isomalto-oligosaccharide. By improving the initial concentration of starch milk, through performing insulation liquification and spraying liquification to prepare a high-concentration maltodextrin solution, by virtue of a synergistic effect of beta-amylase and pullulanase (incisal enzyme) in a process of converting glucoside by saccharifying, the content and the concentration of maltose in liquid glucose are increased; meanwhile, due to the fact that the pullulanase is added, the viscosity of the high-concentration liquid glucose can be reduced, and an optimal substrate environment is provided for alpha-glucosyltransferase, thus the yield of the isomalto-oligosaccharide is improved, and the time of converting glucoside by saccharifying is shortened. The starch resource is wasted due to the fact that a separation and purification technology of the isomalto-oligosaccharide is not enough advanced. According to the method disclosed by the invention, by adopting a nanofiltration membrane technology, the purity of the isomalto-oligosaccharide is increased, crystalline dextrose can be co-produced when high-purity liquid glucose can be produced as a byproduct, and thus the problem of low resource utilization rate is effectively solved.

Description

Technical field [0001] The invention relates to the technical field of production of isomalt oligosaccharides, in particular to a production method for producing high-purity isomalt oligosaccharides by using more than 50% high-concentration corn starch milk as raw materials and high conversion rate. Background technique [0002] Isomaltooligosaccharide (IMO) is a kind of starch sugar. Its main component is isomaltose (IG) combined with α-1,6-glycosidic bond. 2 ), panose (P), isomaltotriose (IG 3 ) And four sugars (including four sugars) and above (G n ) Oligosaccharides. It is a kind of starch syrup (powder) produced by enzymatic method. Isomaltooligosaccharide has the properties of sweet and mellow, low viscosity, heat and acid resistance, non-fermentability, and good moisture retention. In terms of physiological functions, isomalt oligosaccharide has health functions such as improving the structure of the intestinal flora, resisting dental caries, regulating body metabolism, im...

Claims

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Application Information

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IPC IPC(8): C12P19/14
Inventor 杨志强于涛张春泓李涛
Owner 吉林省轻工业设计研究院
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