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Production method for preparing hydrolyzed wheat proteins by fermentation method

A technology of wheat hydrolyzed protein and production method, which is applied in the field of preparing wheat hydrolyzed protein by fermentation method, can solve the problems of affecting the taste and flavor of products, popularization and application, easy damage of amino acids, unstable product quality, etc., and achieves significant social and economic benefits and economical benefits. Significant social benefits and small molecular weight

Inactive Publication Date: 2014-12-17
郑州新威营养技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The product quality of the acid-base hydrolysis method is unstable, and the amino acids produced by hydrolysis are easy to be damaged, which is easy to cause the loss of nutrients; the enzymatic hydrolysis method is currently the main method for the production of wheat hydrolyzed protein, and its disadvantage is that the enzyme used for the hydrolysis of gluten powder, The price is relatively high, and at the same time, the enzymatic hydrolysis products have a bitter taste problem to a large extent, which affects the taste and flavor of the product and the promotion and application; the microbial fermentation method is to decompose the gluten powder through enzymes produced by microbial metabolic activities, and the decomposition is relatively thorough. Rich in small molecular peptides with high nutrition
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Method used

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  • Production method for preparing hydrolyzed wheat proteins by fermentation method
  • Production method for preparing hydrolyzed wheat proteins by fermentation method

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Experimental program
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Effect test

Embodiment 1

[0023] Transfer the activated Aspergillus oryzae strain (Aspergillu) into the liquid seed medium, culture it at 33°C, 200r / min for 36 hours, and use it for fermentation when a uniform small hyphae ball is formed; at the same time, the activated Bacillus subtilis The bacterial strain (Bacillus subtilis) was inoculated in the seed medium, and cultured with shaking at 37°C and 120r / min for about 24h, so that the bacterial cell concentration reached 10 6 -10 8 CFU / mL for use. Take wheat gluten protein powder, add wheat gluten protein powder and water in fermenter and be configured to be 6% by weight percentage concentration, add relative to the growth nutrition bag of wheat gluten protein quality 0.2%, control glucose, sodium chloride in the nutrition bag , potassium dihydrogen phosphate, and dipotassium hydrogen phosphate in a weight ratio of 100:1:1:0.5, adjust the pH to 6.0, then sterilize and cool down to 33°C, and insert the above-mentioned cultivated mature Bacillus subtili...

Embodiment 2

[0025] Transfer the activated Aspergillus oryzae strain (Aspergillu) into the liquid seed medium, culture it at 33°C, 200r / min for 36 hours, and use it for fermentation when a uniform small hyphae ball is formed; at the same time, the activated Bacillus subtilis The bacterial strain (Bacillus subtilis) was inoculated in the seed medium, and cultured with shaking at 37°C and 120r / min for about 24h, so that the bacterial cell concentration reached 10 6 -10 8 CFU / mL for use. Take wheat gluten protein powder, add wheat gluten protein powder and water in fermenter and be configured to be 7% by weight percentage concentration, add relative to the growth nutrition bag of wheat gluten protein quality 0.2%, control glucose, sodium chloride in the nutrition bag , potassium dihydrogen phosphate, and dipotassium hydrogen phosphate in a weight ratio of 100:1.5:1.5:0.6, adjust the pH to 6.5, then sterilize and cool down to 34°C, insert the above-mentioned cultivated mature Bacillus subtili...

Embodiment 3

[0027] Transfer the activated Aspergillus oryzae strain (Aspergillu) into the liquid seed medium, culture it at 33°C, 200r / min for 36 hours, and use it for fermentation when a uniform small hyphae ball is formed; at the same time, the activated Bacillus subtilis The bacterial strain (Bacillus subtilis) was inoculated in the seed medium, and cultured with shaking at 37°C and 120r / min for about 24h, so that the bacterial cell concentration reached 10 6 -10 8 CFU / mL for use. Take wheat gluten protein powder, add wheat gluten protein powder and water in fermenter and be configured to be 8% by weight percentage concentration, add relative to the growth nutrition bag of wheat gluten protein quality 0.2%, control glucose, sodium chloride in the nutrition bag , potassium dihydrogen phosphate, and dipotassium hydrogen phosphate in a weight ratio of 100:2:1:0.3, adjust the pH to 7.0, then sterilize and cool down to 35°C, and insert the above-mentioned cultivated mature Bacillus subtili...

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Abstract

The invention discloses a production method for preparing hydrolyzed wheat proteins by a fermentation method, and belongs to the technical field of feed additive production. According to the method, wheat gluten protein powder serves as a raw material and is hydrolyzed into a short peptide chain under the action of protein enzyme preparations such as endo protease and carboxy-peptidase generated by mixed fermentation of bacillus subtilis and aspergillus oryzae, and hydrophobic amino acid at the tail end of the short peptide chain is cut off, so that proteolysis and debittering of the hydrolyzed wheat proteins can be realized at one step by the fermentation method. Compared with a traditional proteolysis method, the production method has the advantages that production cost is low, reaction conditions are moderate, technical parameters are reliable, product quality is stable, bitter taste is avoided, indicators such as product flavor and colors are superior to enzymatic hydrolysis, the hydrolyzed wheat proteins are small in molecular weight, fine in solubility and high in glutamine content, plasma proteins can be replaced, the digestibility of weaning young animals is obviously improved after applications are added, and stress and diarrhea are obviously avoided.

Description

technical field [0001] The invention relates to the field of comprehensive development and application of plant protein, in particular to a production method for preparing wheat hydrolyzed protein by fermentation, which belongs to the technical field of feed additive production. Background technique [0002] The protein molecular weight of wheat gluten protein is relatively large, contains more hydrophobic amino acids and uncharged amino acids, has a large area of ​​hydrophobic interaction in the molecule, and has low solubility. The application effect is not ideal or the application range is limited. As a high-quality antigen-free vegetable protein source, wheat hydrolyzed protein is widely used in piglet feed, high-grade aquatic product feed, calf milk substitute and pet feed. The production methods of wheat hydrolyzed protein mainly include chemical hydrolysis, enzymatic hydrolysis and microbial fermentation. The product quality of the acid-base hydrolysis method is uns...

Claims

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Application Information

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IPC IPC(8): C12P21/00C12R1/69
Inventor 张新武张子峰
Owner 郑州新威营养技术有限公司
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