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Method for producing nanometer particles through nanometer emulsification technology

A nano-particle and nano-technology, applied in the field of nano-particle preparation, can solve the problems of insolubility of curcumin in water, low absorption rate and narrow application range, and achieve the effects of improving bioavailability, low production cost and high nutritional value.

Active Publication Date: 2014-12-24
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problems that curcumin is insoluble in water, has low absorption rate and narrow application range, the present invention provides a method for producing nanoparticles using nanoemulsification technology

Method used

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  • Method for producing nanometer particles through nanometer emulsification technology

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specific Embodiment approach 1

[0018] Specific implementation mode one: as figure 1 As shown, this embodiment provides a method for producing nanoparticles using nanoemulsion technology, and the production steps are as follows:

[0019] (1) Dissolve whey protein isolate (protein content ≥90%) in 0.05 M, pH=7 phosphate buffer solution, stir for 2 h, and prepare 1.25 g / 100 mL whey protein solution.

[0020] (2) Heat medium-chain triglycerides to 110°C, then add curcumin into the hot medium-chain triglycerides, and make it fully dissolve to prepare 0.1 g / 100 mL curcumin oil solution.

[0021] (3) Mix curcumin medium-chain triglyceride oil solution cooled to room temperature with whey protein aqueous solution, and use a high-shear emulsifier to stir at 11,000 rpm / min for 5 minutes to prepare a coarse emulsion. The oil phase (medium chain triglycerides) in the emulsion is 5% (v / v), and the water phase (whey protein solution) is 95% (v / v).

[0022] (4) The coarse emulsion was circulated and homogenized three ti...

specific Embodiment approach 2

[0023] Specific embodiment two: This embodiment provides a method for producing nanoparticles using nanoemulsification technology, and its production steps are as follows:

[0024] (1) Dissolve whey protein isolate (protein content ≥90%) in 0.05M, pH=7 phosphate buffer solution, stir for 3 hours, and prepare 2.5 g / 100 mL whey protein solution.

[0025] (2) Heat medium-chain triglycerides to 120°C, then add curcumin into the hot medium-chain triglycerides and dissolve them fully to prepare a 0.5 g / 100 mL curcumin oil solution.

[0026] (3) Mix curcumin medium-chain triglyceride oil solution cooled to room temperature with whey protein aqueous solution, and use a high-shear emulsifier to stir at 12000 rpm / min for 6 minutes to prepare a coarse emulsion. The oil phase (medium chain triglycerides) in the emulsion accounts for 10% (v / v), and the water phase (whey protein solution) accounts for 90% (v / v).

[0027] (4) The coarse emulsion was circulated and homogenized three times un...

specific Embodiment approach 3

[0028] Specific Embodiment Three: This embodiment provides a method for producing nanoparticles using nanoemulsification technology, and its production steps are as follows:

[0029] (1) Dissolve whey protein isolate (protein content ≥90%) in 0.05M, pH=7 phosphate buffer solution, stir for 5 hours, and prepare 7 g / 100 mL whey protein solution.

[0030] (2) Heat medium-chain triglycerides to 140°C, then add curcumin into the hot medium-chain triglycerides and dissolve them fully to prepare a 1 g / 100 mL curcumin oil solution.

[0031] (3) Mix curcumin medium-chain triglyceride oil solution cooled to room temperature with whey protein aqueous solution, and use a high-shear emulsifier to stir at 13,000 rpm / min for 10 minutes to prepare a coarse emulsion. The oil phase (medium chain triglycerides) in the emulsion accounts for 30% (v / v), and the water phase (whey protein solution) accounts for 70% (v / v).

[0032] (4) The coarse emulsion was circulated and homogenized three times un...

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Abstract

The invention discloses a method for producing nanometer particles through a nanometer emulsification technology. Bovine whey protein is served as a surface active agent and medium chain triglycerides are served as oil phases to produce nanometer particle carried fat soluble bioactive substance curcumin. According to the nanometer emulsified liquid prepared in the invention, the average grain diameter of the nanometer particles in a dispersed phase is about 200nm, the stability is good and the storage stability is good under high temperature and high ion strength, and the curcumin is protected. Human body intestinal epithelial cells are simulated by constructing Caco-2 monolayer cells to inspect influences of a curcumin nanometer emulsification system to the curcumin absorption efficiency. A research result shows that the absorbing capacity of the curcumin is improved to 35% from 15% after the curcumin is carried by the nanometer emulsification particles within one hour, the absorption rate is improved to 7.07*10-5 centimeter per second.

Description

technical field [0001] The invention relates to a method for preparing nanoparticle, in particular to a method for preparing curcumin nanoparticle using bovine whey protein and medium-chain triglyceride as raw materials and adopting nanoemulsification technology. Background technique [0002] Curcumin is a yellow pigment extracted from turmeric, which belongs to phenolic substances. Many studies have shown that curcumin has biological activities such as anti-inflammation, anti-oxidation, lowering blood fat, inhibiting complications of type 2 diabetes, inhibiting thrombosis and myocardial infarction, and resisting various cancers. Although the pharmacological effects of curcumin are very clear, its clinical application is greatly limited due to its own factors. First of all, curcumin is almost insoluble in water, its solubility in water is extremely low (11 ng / mL or 30 nmol / L), it is insoluble under acidic conditions, and it is unstable under neutral and alkaline conditions....

Claims

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Application Information

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IPC IPC(8): A61K9/107A61K31/12A61K47/42A61K47/14A61P29/00A61P39/06A61P3/06A61P3/10A61P9/10A61P7/02A61P35/00
Inventor 马莺李明程金菊
Owner HARBIN INST OF TECH
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