Method for preparing reducing sugar by promoting lignocellulase to hydrolyze by ultrasonic pre-treatment in pressurized hot water

A lignocellulosic enzyme and lignocellulose technology, which is applied in the fields of green chemistry and clean energy, can solve the problems of complex ammonia fiber blasting pretreatment process, difficult product separation, and high equipment requirements, achieving no high temperature, simple follow-up treatment, and equipment less demanding effect

Inactive Publication Date: 2015-01-07
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for ultrasonic pretreatment in pressurized hot water to promote enzymatic hydrolysis of lignocellulose to prepare reducing sugar, to solve the problems of acid-base pretreatment corrosion equipment, high requirements for equipment, difficult product separation and waste liquid treatment, Supercritical carbon dioxide pretreatment and steam explosion pretreatment have harsh conditions such as high temperature and high pressure and high energy consumption. The ammonia fiber blasting pretreatment process is complicated, the recovery cost is high, and the environment is polluted.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Weigh 20g of deionized water and 1g of corn stalks according to the mass ratio of 20:1, mix them into the reactor, and pour CO into the reactor. 2 , kept at 5MPa, placed in an ultrasonic tank, and kept at 80°C, 40kHz, 600W for 60min. After the reaction, the pressure was quickly released to normal pressure, and then the corn stalks were taken out and dried to constant weight. Put 1g of pretreated corn stalks, 0.7g of cellulase with an activity of 4000U / g, and 50ml of acetic acid-sodium acetate buffer solution with a pH value of 4.8 and a concentration of 0.2M into a 100ml Erlenmeyer flask. Hydrolyze in a water-bath shaking table for 48 hours, filter and centrifuge the product, and carry out ultraviolet analysis on the liquid phase product, and the yield of reducing sugar is 27.6%.

Embodiment 2

[0017] Weigh 50g of deionized water and 1g of corn stalks according to the mass ratio of 50:1, mix them into the reactor, and pour N into the reactor. 2 , kept at 10MPa, placed in an ultrasonic tank, and kept at 40kHz, 600W, and 120°C for 60min. After the reaction, the pressure was quickly released to normal pressure, and then the corn stalks were taken out and dried to constant weight. Put 1g of pretreated corn stalks, 0.7g of cellulase with an activity of 4000U / g, and 50ml of acetic acid-sodium acetate buffer solution with a pH value of 4.8 and a concentration of 0.2M into a 100ml Erlenmeyer flask. The product was hydrolyzed in a water-bath shaker for 48 hours, the product was filtered and centrifuged, and the liquid phase product was subjected to ultraviolet analysis, and the yield of reducing sugar was 26.8%.

Embodiment 3

[0019] Weigh 20g of deionized water and 1g of corn cob according to the mass ratio of 20:1, mix them into the reactor, and pour CO into the reactor. 2 , kept at 5MPa, placed in an ultrasonic tank, and kept at 59kHz, 90W, and 80°C for 120min. After the reaction, the pressure was quickly released to normal pressure, and then the corn stalks were taken out and dried to constant weight. Put 1g of pretreated corn stalks, 0.7g of cellulase with an activity of 4000U / g, and 50ml of acetic acid-sodium acetate buffer solution with a pH value of 4.8 and a concentration of 0.2M into a 100ml Erlenmeyer flask. The product was hydrolyzed in a water-bath shaker for 48 hours, the product was filtered and centrifuged, and the liquid phase product was subjected to ultraviolet analysis, and the yield of reducing sugar was 26.3%.

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Abstract

The invention belongs to the technical field of green chemistry and clean energy and relates to a method for preparing reducing sugar by promoting lignocellulase to hydrolyze by ultrasonic pre-treatment in pressurized hot water. The method comprises the following steps: mixing deionized water with lignocellulose in a mass ratio of (10-100) to 1 and introducing 3-10MPa carbon dioxide or nitrogen at 80-120 DEG C; applying an ultrasonic field with the frequency being 40-60kHz and the power being 90-600W for 10-120 minutes; releasing the pressure to normal pressure within 2-10 minutes and drying; adding an 0.2M acetic acid-sodium acetate buffer solution with the pH of 4.8 to the lignocellulose, wherein the mass ratio of the buffer solution to the lignocellulose is 50 to 1; and then adding cellulase, and hydrolyzing to obtain the reducing sugar, wherein the mass ratio of the cellulase to the lignocellulose is (0.5-1.2) to 1. The method provided by the invention is time-saving, mild in condition, low in energy consumption, simple in process, low in cost, green and free from pollution.

Description

technical field [0001] The invention belongs to the technical field of green chemistry and clean energy, and relates to a method for preparing reducing sugar by promoting lignocellulosic enzymatic hydrolysis through ultrasonic pretreatment in pressurized hot water. Background technique [0002] Lignocellulose is composed of hemicellulose, cellulose and lignin, mainly derived from crop straw, trees and weeds. As a kind of biomass resource, it has become the focus of renewable energy and resource utilization because of its renewable and huge output. Direct combustion is the traditional form of lignocellulose utilization, which has low energy efficiency and serious environmental pollution. Hydrolyzing lignocellulose to reducing sugars through chemical or biological methods, and then fermenting to produce fuel ethanol is an important part of alternative energy and an efficient way to utilize lignocellulosic biomass resources. Enzymatic hydrolysis of lignocellulose to prepare r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14
Inventor 李国民银光照刘一凡郝刘丹银建中
Owner DALIAN UNIV OF TECH
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