Method for synthesizing trehalose under catalysis of co-immobilized double enzymes

A technology of trehalose and trehalose synthase, which is applied in the fields of immobilization on/in organic carriers, chemical industry, sustainable manufacturing/processing, etc., can solve the problems of low utilization rate and high price of catalytic raw materials and substrates, and achieve The effect of reducing raw material costs, shortening production cycle, and broad application prospects
CN104328107AInactive Publication Date: 2015-02-04NANJING HAIHE BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NANJING HAIHE BIOTECH
Publication Date
2015-02-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for synthesizing trehalose under catalysis of co-immobilized double enzymes. According to the method, recombinant escherichia coli capable of synthesizing high-temperature-resisting trehalose synthase in a great quantity by fermentation culture is subjected to permeability treatment, and beta-amylase is added; chitosan and sodium alginate are used for co-immobilizing to form micro-spheres; starch with the weight content of 25%-30% is catalyzed by alpha-amylase and then is subjected to a contact reaction with the micro-spheres to generate the trehalose, wherein after the micro-spheres are repeatedly utilized for 8-16 times, the conversion rate of the starch can be up to 60%-65%. By adopting the method provided by the invention, the high-quality trehalose can be provided in a large-scale, low-cost and high-efficiency manner.
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Description

technical field

[0001] The invention relates to a method for synthesizing trehalose catalyzed by co-immobilized double enzymes, belonging to the technical field of functional sugar alcohol synthesis. It specifically relates to a method for catalytically synthesizing trehalose by using starch as a raw material. Background technique

[0002] Trehalose is a non-reducing disaccharide formed by combining two glucose molecules with α,α-1,1 glycosidic bonds. Trehalose has no hemiacetal hydroxyl group and is chemically stable. Since Wiggers first extracted trehalose from ergot bacteria in 1832, trehalose has been widely found in animals, plants and microorganisms in nature, such as bacteria, archaea, yeast, mushrooms and invertebrates. Because trehalose can form a unique protective film on the cell surface under harsh environmental conditions such as high temperature, high cold, high osmotic pressure, and dehydration, effectively protecting protein molecules from denaturation and i...

Claims

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