Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method of nitrocellulose diacetate nanofiber membrane capable of adsorbing and desorbing proteins

A technology of cellulose diacetate and nitrodiacetic acid is applied in the field of protein separation and purification, which can solve the problems of complex membrane pore structure, low processing capacity, long operation time, etc., and achieves optimized microstructure, low fiber size, and improved adsorption. Effect

Active Publication Date: 2016-08-17
BEIJING INSTITUTE OF TECHNOLOGYGY +1
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are several defects in the above types: (1) under high pressure driving, the membrane rigidity is insufficient and easy to compact; (2) the membrane pore structure is complex and the mass transfer effect is poor; (3) the affinity ligand is expensive; (4) ) low throughput and long operating time
[0004] Cellulose diacetate is one of the cellulose derivatives that can be used in various technical fields. It has the advantages of large output, low cost, and stable physical and chemical properties. However, the material has high acetyl group content and no specific adsorption on proteins. Thus limiting its expansion in the field of separation and purification applications
Although traditional nitrocellulose has obvious adsorption to protein, it has disadvantages such as poor material toughness, high hydrophobicity, and narrow range of spinning solvents.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of nitrocellulose diacetate nanofiber membrane capable of adsorbing and desorbing proteins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Weigh 1g of cellulose diacetate, 6mL of acetone and 3mL of DMAc solvent (solvent volume ratio 2:1) into a 20mL Erlenmeyer flask, and stir magnetically at room temperature for 6-8h to form a transparent homogeneous spinning solution; The spinning solution was left to defoam for 4 hours, then poured into a 20mL syringe equipped with a No. At 15-20kV, the needle droplet rapidly formed a Taylor cone, and a cellulose diacetate nanofiber film was formed on the conductive receiving plate covered with aluminum foil. Then, soak the membrane in deionized water for 24 hours to remove residual solvents and impurities, and dry it in an oven at 60° C. to obtain pure cellulose diacetate nanofiber membrane 1 .

[0038] Weigh 0.15g of bovine serum albumin and dissolve it in 0.01M disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution (pH=7.2), immerse the prepared membrane in the protein solution, shake and adsorb for 4h, centrifuge the solution at 10000 rpm for 5min, a...

Embodiment 2

[0041] Step 1. Dissolve 8g of cellulose diacetate in glacial acetic acid and stir rapidly for 2 hours to fully dissolve and activate it;

[0042] Step 2. After dissolving, add the hydrolyzate (mixed with water / sulfuric acid / glacial acetic acid in a mass ratio of 45 / 30 / 2) in a water bath at 18°C, raise the temperature to 80°C, perform hydrolysis treatment for 3 hours, neutralize and precipitate, wash with water until neutral, Obtain hydrolyzed cellulose diacetate;

[0043] Step 3. Weigh the cellulose diacetate (acetyl substitution degree is 1.8) and nitrating agent (mixing fuming nitric acid and dichloromethane at a mass ratio of 35 / 65) obtained in step 2 and add them into a three-necked flask, and carry out nitration treatment at room temperature for half Hour;

[0044]Step 4, add ethanol after nitration, wash repeatedly to neutrality, obtain nitrocellulose diacetate;

[0045] Step 5, take by weighing 1g of the nitrocellulose diacetate gained in step 4 (the degree of nitro s...

Embodiment 3

[0051] Step 1. Dissolve 8g of cellulose diacetate in glacial acetic acid and stir rapidly for 2 hours to fully dissolve and activate it;

[0052] Step 2. After dissolving, add hydrolyzate (water / sulfuric acid / glacial acetic acid mixed in mass ratio 45 / 30 / 2) in a water bath at 18°C, heat up to 80°C, hydrolyze for 3 hours, neutralize and precipitate, wash with water until neutral, Obtain hydrolyzed cellulose diacetate;

[0053] Step 3: Weigh cellulose diacetate (acetyl substitution degree is 1.8) and nitrating agent (mixing fuming nitric acid and dichloromethane at a mass ratio of 45 / 55) obtained in step 2 into a three-necked flask, and carry out nitration treatment at room temperature for half an hour ;

[0054] Step 4, add ethanol after nitration, wash repeatedly to neutrality, obtain nitrocellulose diacetate;

[0055] Step five, take by weighing 1.198g of the nitrocellulose diacetate gained in step four (the degree of nitro substitution is 0.87) and be dissolved in 6.247gDM...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
adsorption capacityaaaaaaaaaa
adsorption capacityaaaaaaaaaa
adsorption capacityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a nitrocellulose diacetate nano-fiber membrane capable of adsorbing and desorbing protein and aims to provide a modified nitrocellulose diacetate formula and a preparation technology of a nano-fiber membrane, and prepare a nitrocellulose diacetate nano-fiber membrane with high dimension distribution and high protein adsorbing capacity. Nitrocellulose diacetate is added into a hydrolysis system to remove part of acetyl; nitration is performed through nitrosonitric acid / methylene dichloride to obtain nitrocellulose diacetate, the obtained nitrocellulose diacetate is dissolved in a novel bi-phase solvent system, and electrostatic spinning is carried out to prepare the nitrocellulose diacetate nano-fiber membrane. The prepared nano-fiber membrane shows favorable dimension distribution, can adsorb protein of 210 mg / g, the adsorbed protein is easy for elution, and the desorption rate can be more than 80%. The adsorption-desorption process is circulated for three times and the desorption rate can still be kept more than 80%, so that the application requirements in protein purification and separation can be satisfied.

Description

technical field [0001] The invention relates to a nitrocellulose diacetate nanofiber membrane capable of adsorbing and desorbing proteins and its preparation technology, belonging to the technical field of protein separation and purification. Background technique [0002] In recent years, electrospinning, as a new nano-fabrication technology, has attracted widespread attention from scientists at home and abroad. This technology refers to the method that under the action of high-voltage electrostatic force, the polymer solution droplets overcome the surface tension to form a Taylor cone, and the polymer is fully stretched, solidified with the volatilization of the solvent, and finally forms a method of ultrafine fibers. During the spinning process, the charged droplets are subjected to the force of the electric field. When the voltage reaches a critical value, the electrostatic force is greater than the surface tension, and the droplets form continuous fibers and are collecte...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): D04H1/728D04H1/425D01D1/02
CPCD04H1/425D04H1/728
Inventor 邵自强兰天顾慕珈王文俊王飞俊张仁旭赵利斌
Owner BEIJING INSTITUTE OF TECHNOLOGYGY