Preparation method of lactobacillus plantarum capsule for poultry

A technology of Lactobacillus plantarum and capsules, applied in the fields of application, animal feed, animal feed, etc., can solve the problems of unfavorable industrialization and application of microcapsule products, destruction of lactic acid bacteria, poor tolerance of gastric environment, etc., and achieve the purpose of inhibiting the growth of pathogenic bacteria, Enhance the effect of gastric acid resistance and good enteric solubility

Inactive Publication Date: 2015-03-04
邵素英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] To sum up, the existing microcapsule coating technology will cause greater damage to lactic acid bacteria during its implementation, or the micr

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The breeding process of Lactobacillus plantarum CGMCC No.9405 used in the present invention is as follows:

[0033] Original starting strain → test tube activation → diethyl sulfate (DES) mutagenesis → nitrosoguanidine (NTG) mutagenesis → plasma mutagenesis → plate primary screening → shake flask secondary screening → passage stability test.

[0034] The starting bacterial strain adopted in the present invention is in the MRS glucose medium, and its production rate of lactic acid is 1.5g / L / d, almost stops growing when the pH of the medium is 3.5, and the decomposition rate to sodium nitrite is 0.34mg / h / kg cabbage. The starting strain was collected by Li Zheng from the silage feed of the fattening sheep farm in Yanchi County, Ningxia, and the collection time was September 15, 2013.

[0035] In order to improve its lactic acid production rate, acid resistance and nitrite decomposition rate, the strain was mutated by using DES and NTG techniques in sequence. After the mu...

Embodiment 2

[0066] Lactobacillus plantarum capsules are composed of wall material, Lactobacillus plantarum, freeze-drying protective agent, stachyose, and hickory chick enzymatic hydrolysis powder.

[0067] The wall material is composed of enzymatically hydrolyzed soybean protein isolate, chitosan, xanthan gum, carrageenan, glycerin and trehalose; Chitosan 1%, xanthan gum 3%, carrageenan 0.1%, glycerin 0.5%, trehalose 0.5%.

[0068] The preparation method of enzymatically hydrolyzing soybean protein isolate is as follows: prepare a solution of soybean protein isolate with a concentration of 10-13%, heat it to 30-33°C, adjust the pH to 3, add acidic protease with 0.2% weight of soybean protein isolate and heat it for 1.5 hours. The hydrolyzed solution is spray-dried to obtain enzymatically hydrolyzed soybean protein isolate.

[0069] Lactobacillus plantarum is a strain with the preservation number CGMCC NO.9405.

[0070] The preparation method of the hickory chick enzymolysis powder is a...

Embodiment 3

[0080] Lactobacillus plantarum capsules are composed of wall material, Lactobacillus plantarum, freeze-drying protective agent, stachyose, and hickory chick enzymatic hydrolysis powder.

[0081] The wall material is composed of enzymatically hydrolyzed soybean protein isolate, chitosan, xanthan gum, carrageenan, glycerin and trehalose; Chitosan 2%, xanthan gum 1%, carrageenan 0.5%, glycerin 1%, trehalose 2%.

[0082] The preparation method of enzymatically hydrolyzing soybean protein isolate is as follows: prepare a solution of soybean protein isolate with a concentration of 10-13%, heat it to 42-45°C, adjust the pH to 5, add acidic protease with 1% of the weight of soybean protein isolate, and keep it for 0.5 hours for enzymolysis. The hydrolyzed solution is spray-dried to obtain enzymatically hydrolyzed soybean protein isolate.

[0083] The preparation method of hickory chick enzymolysis powder is as follows:

[0084] (1) Morchella fruiting bodies are dried and pulverized;...

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Abstract

The invention relates to a preparation method of a lactobacillus plantarum capsule for poultry, and belongs to the technical field of microbial feed additives. The lactobacillus plantarum capsule consists of a wall material, lactobacillus plantarum, a freeze-drying protective additive, stachyose and toadstool enzymolysis powder, wherein the wall material consists of enzymolysis soybean protein isolate, chitosan, xanthan gum, carrageenan, glycerinum and mycose. When the substances are prepared into a mixed solution, the concentration of the enzymolysis soybean protein isolate is 7-10 percent; the concentration of the chitosan is 1-2 percent; the concentration of the xanthan gum is 1-3 percent; the concentration of the carrageenan is 0.1-0.5 percent; the concentration of the glycerinum is 0.3-1 percent; and the concentration of the mycose is 0.5-2 percent. Through adoption of the lactobacillus plantarum capsule prepared by using the method, the gastric acid resistance of the capsule is enhanced; very high enteric solubility is achieved; the growth of pathogenic bacteria can be effectively restrained; the immunity is enhanced; the morbidity of the poultry is lowered; the breeding quality is enhanced; and the breeding benefit is increased.

Description

technical field [0001] The invention relates to a preparation method of lactic acid bacteria microcapsules, belonging to the technical field of microbial feed additives. Background technique [0002] The wide application of antibiotics and other antibacterial drugs in animal husbandry has greatly promoted the development of animal husbandry. At present, more than 95% of premix products have added antibacterial drugs. But its disadvantages are also becoming more and more obvious: serious drug residues in animal husbandry products directly endanger human health; the abuse of antibiotics leads to drug resistance of pathogenic bacteria, resulting in the spread and spread of drug-resistant strains; at the same time, animal flora imbalance, animal immunity decline, the breeding environment deteriorates, and diseases increase; causing a series of serious problems such as animal endogenous infection and double cross-infection. The European Union began to ban the addition of antibi...

Claims

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Application Information

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IPC IPC(8): A23K1/16
Inventor 邵素英李建树
Owner 邵素英
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