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A kind of fairy grass polysaccharide gel ball and its preparation method and application

A technology of celery polysaccharide and gel balls, which is applied in the directions of polysaccharide/gum-containing food ingredients, applications, dairy products, etc., can solve the problems of not realizing the improvement of gastric acid stability and intestinal targeted release effect of probiotics or embedded components , to achieve the effect of improving the absorption capacity of the small intestine, high embedding stability, and improving gastric acid resistance.

Active Publication Date: 2022-07-01
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The publication number is CN 111357817 A, titled "An active health-preserving yogurt jelly jelly and its preparation method". The jelly jelly product with antioxidant effect is obtained by compounding jelly powder and yogurt, but the jelly polysaccharide-protein compound Gel or jelly did not achieve improved gastric acid stability and intestinal targeted release of probiotics or entrapped ingredients

Method used

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  • A kind of fairy grass polysaccharide gel ball and its preparation method and application
  • A kind of fairy grass polysaccharide gel ball and its preparation method and application
  • A kind of fairy grass polysaccharide gel ball and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1 Determination of the composition of the polysaccharide of sagebrush by high performance liquid chromatography

[0070] Specific steps are as follows:

[0071] (1) Preparation of the sample loading solution for the polysaccharide sample of the grass to be tested:

[0072] The polysaccharide of the grass polysaccharide was purchased from Wuhan Yuancheng Gongchuang Technology Co., Ltd. (CAS: 542-40-5, standard: enterprise standard, grade: food grade, content: 99%). Put 10 mg of grass polysaccharide sample into a 10 ml ampoule, add 2.0 mL of 2 mol / L trifluoroacetic acid into a 5 mL ampoule, seal the tube, hydrolyze with acid at 110 °C for 8 h, take out the trifluoroacetic acid to evaporate dry, and add 2.0 ml of water to reconstitute.

[0073] Sample solution derivatization: accurately pipette 250uL sample solution into 5mL EP tube, add 250uL 0.6 mol / L NaOH, 500 uL 0.4mol / L 1-phenyl-3-methyl-5-pyrazolone-methanol, react at 70℃ for 1h . Cool in cold water for 10...

Embodiment 2

[0081] Example 2 Preparation of celery microgel spheres by inverse emulsion method

[0082] Accurately weigh 50 mg of the polysaccharide from the grass, dissolve it in 0.5 mL of pH 3 water, and vortex until the polysaccharide is completely dissolved; accurately weigh 5 mg of ferric chloride metal ions. The sagebrush polysaccharide solution is mixed with metal ions.

[0083]Weigh 40 g of liquid paraffin and 1 g of Span80 into a 50 mL beaker, add a fully mixed solution of polysaccharide and iron, and stir magnetically for 1 h to fully crosslink polysaccharide and metal ions.

[0084] After 1 h, centrifuge at 1000 rpm for 2 min, separate the oil phase and the water phase, discard the upper oil phase, wash the microspheres with n-hexane and methanol each for 3 times in the lower water phase, and finally reconstitute the microgel spheres to 1 mL pH3. Distilled water.

[0085] The fairy grass microgel spheres were scanned by scanning electron microscope SEM. figure 2 As shown, t...

Embodiment 3

[0087] Example 3 Preparation of gel microspheres embedded with probiotics

[0088] 0.5 mL of 6×10 10 Cfu / mL probiotics were centrifuged at 3000 rpm at 4°C for 10 min, washed twice with normal saline, and reconstituted into 0.5 mL of normal saline. Dissolve 50 mg of grass polysaccharide in 0.5 mL of pH3 water, vortex until the polysaccharide is completely dissolved; accurately weigh 5 mg of ferric chloride, fully mix the grass polysaccharide solution, bacterial liquid and metal ions, and add 40 g of liquid paraffin. and 1 g Span80 in a 50 mL beaker, magnetically stirred for 1 h to fully cross-link the polysaccharides and metal ions, and centrifuged at 1000 rpm for 2 min after 1 h to separate the oil phase and the water phase, discard the upper oil phase, and use n-hexane for the lower water phase. The microspheres were washed three times with alkane and methanol each, and finally the microgel spheres embedded with probiotics were reconstituted into 1 mL of distilled water wit...

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Abstract

The present invention provides a gelatinous ball of curcuma polysaccharide as well as a preparation method and application thereof. The round gel ball formed by curcuma polysaccharide has good gastric acid resistance, effectively protects the stability of active ingredients in gastric acid, and is safe in the intestinal tract. It can achieve intestinal-targeted release of active ingredients, effectively improve the utilization rate of probiotics or functional ingredients, help probiotics adhere to intestinal mucus and colonize the intestine for a long time, and improve the intestinal release and absorption capacity of other active ingredients.

Description

technical field [0001] The present invention relates to the field of food biotechnology. In particular, the present invention relates to Curcuma polysaccharide gel balls and their preparation method and application. Background technique [0002] Fairy grass, also known as fairy grass, jelly grass, scientific name Mesonachinensis Benth, English name MesonaBlume, produced in Taiwan, Fujian, Zhejiang, Jiangxi, Guangdong, Guangxi and other places. It is a kind of medicinal and edible plant rich in grass polysaccharide (Xiancao glue) and flavonoids. Xiancao is astringent, sweet and cold in nature, has the effects of clearing heat and quenching thirst, cooling blood and relieving heat, and can cure heat stroke, high blood pressure, kidney disease and diabetes. Foods processed with celery as raw materials have been reported to have certain antioxidant properties, among which celery health tea has the effect of scavenging superoxide anions, and celestial honey has the effect of sc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L29/206
CPCA23L29/206A23L29/294A23P10/30A23L33/105A23L33/15A23L33/10A23L33/19A23L33/17A23L33/135A23L2/52A23C9/1307A23C9/1322A23C19/09A23C19/0921A61K9/06A61K47/46A23V2002/00A23V2200/30A23V2250/51A23V2250/1592A23V2200/3204A23V2250/2112A23V2250/21
Inventor 李媛张慧娟刘斌王启蒙李星侯国华梁爽闫恵苓
Owner CHINA AGRI UNIV
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