Enzymolysis saccharification processing method of straw

A technology of saccharification treatment and enzymatic hydrolysis, which is applied in the fields of biofuel and fermentation, can solve the problems of increasing the difficulty of enzymatic hydrolysis, restricting direct contact with cellulose, low enzymatic hydrolysis and saccharification rate, etc. Improve the effect of enzymatic hydrolysis and saccharification, and improve the effect of enzymatic hydrolysis efficiency

Inactive Publication Date: 2015-04-29
XINXIANG MEDICAL UNIV
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AI Technical Summary

Problems solved by technology

[0004] The main components of straw are cellulose (about 42%), hemicellulose (about 32%) and lignin (about 8%), and cellulose is dehydrated glucose and linked by β-1,4 glucosidic bonds The linear polymer of cellulose, the cellulose molecules are linked together by a large number of hydrogen bonds to form cellulose bundles of crystal structure, the cellulose bundles are mainly surrounded by hemicellulose, followed by the ring sheath of lignin, thus forming a The natural complex and dense structure limits the direct contact between cellulase and cellulose, increases the difficulty of enzymatic hydrolysis, leads to low enzymatic hydrolysis and saccharification rate, and restricts the industrial development of cellulosic ethanol

Method used

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  • Enzymolysis saccharification processing method of straw

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Enzymatic hydrolysis and saccharification of straw

[0023] Sorghum stalks are dried in the sun, pulverized, and sieved with a 60-mesh sieve to obtain sorghum stalk powder. Take 10g of sorghum straw powder and add it to a conical flask, add 100mL of dilute hydrochloric acid with a mass concentration of 1.0%, then place it in a sterilized autoclave, and carry out acidification reaction at a temperature of 120°C. The sorghum stalks were taken out and cooled naturally at room temperature to obtain sorghum straw slurry.

[0024] Add 0.25 g of cellulase (2.5 g per 100 g of straw), 0.2 g of xylanase (2.0 g per 100 g of straw), 0.375 mmol of magnesium chloride (2.5 mmol / L), 1.5 mL of Tween 80 (1 %) and 50 mL of distilled water to obtain an enzymatic hydrolysis slurry with a liquid-solid ratio of 15:1, adjust the pH value to 5.0, and then place it in a water bath for enzymatic hydrolysis and saccharification reaction at 50°C for 48 hours. After the reaction is comp...

Embodiment 2

[0032] Example 2: Test of straw saccharification effect

[0033] 1. Effect of acidification reaction on straw saccharification effect

[0034] According to the enzymatic saccharification treatment method described in Example 1, sulfuric acid, sulfurous acid, formic acid, and sodium hydroxide with a mass concentration of 1.0% were used to replace the dilute hydrochloric acid used in Example 1, and the acidification reaction was carried out, and the enzymatic The concentration of reducing sugar after desaccharification treatment is shown in Table 1. The test results show that the acidification reaction with dilute hydrochloric acid has the best saccharification effect.

[0035] Table 1

[0036] sour

Dilute hydrochloric acid

sulfuric acid

Sulfurous acid

formic acid

NaOH

reducing sugar concentration

29.1g / L

22.5g / L

4.2g / L

18.9g / L

22.1g / L

[0037]According to the enzymatic saccharification treatment method describe...

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Abstract

The invention discloses an enzymolysis saccharification processing method of straw. The enzymolysis saccharification processing method comprises the following steps: 1) drying straw in the sun, crushing the dried straw, and sieving the crushed straw by a sieve so as to obtain straw powder; 2) adding the straw powder in a diluted hydrochloric acid to perform an acidizing reaction, and enabling the temperature to be lowered to a room temperature when the reaction ends so as to obtain a straw serosity; 3) adding cellulose, xylanase, magnesium chloride, Twain 80 and distilled water in the straw serosity so as to obtain an enzymolysis serosity, and then performing an enzymolysis saccharification reaction. The enzymolysis saccharification processing method of straw, which is disclosed by the invention, can obviously improve the enzymolysis saccharification effect of the straw.

Description

technical field [0001] The invention relates to an enzymatic saccharification treatment method of straw, in particular to an enzymatic saccharification treatment method for increasing the saccharification rate of straw. Background technique [0002] At present, human beings are facing global problems such as resource scarcity, energy shortage and environmental pollution. Many countries are actively exploring and in-depth research on new energy sources. Among them, cellulosic ethanol, as a new type of renewable clean energy, is becoming more and more popular. Pay attention to. [0003] Cellulosic ethanol is fuel ethanol obtained by enzymatic hydrolysis, saccharification, and fermentation of fiber materials such as crop hulls, stems, leaves, and straws. my country is a large agricultural country, with an annual output of more than 700 million tons of crop straw, ranking first in the world, which can provide sufficient raw material base for the production of fuel ethanol. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12P19/14
CPCC12P7/10C12P19/14Y02E50/10
Inventor 康静冯冲刘涌涛毛会丽王燕胡涣涣
Owner XINXIANG MEDICAL UNIV
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