Liquid composite microbial agent and production method thereof

Abstract

The invention provides a liquid composite microbial agent which comprises an active strain and a culture medium, wherein the comprises the following materials in percentage by weight: 90%-95% of sterile water, 1.0%-2.5% of corn meal, 1.0%-2.5% of bean cake meal, 0.1%-1.5% of fish meal, 0.02%-0.5% of corn starch, 0.005%-0.07% of magnesium sulfate, 0.03%-0.05% of potassium dihydrogen phosphate, 0.02%-0.5% of light calcium carbonate and 0.002%-0.1% of sodium hydroxide; the active strain comprises bacillus amyloliquefaciens, bacillus subtilis and bacillus licheniformis. When liquid culture is carried out, the volume of each type of active strains accounts for 1%-5% of that of the culture medium when each type of active strains are respectively inoculated to the culture medium; when three types of fermented bacterium solutions are compounded to form a microbial agent, the volume ratio of a bacillus amyloliquefaciens solution is 60%-80%, the volume ratio of a bacillus subtilis solution is 10%-20%, and the volume ratio of a bacillus licheniformis solution is 10%-20%. The liquid composite microbial agent provided by the invention is low in cost and can be used for inhibiting the mosses and purifying the water environment.

Description

technical field [0001] The invention relates to the field of ecological aquaculture, in particular to a liquid composite microbial bacterial agent and a production method thereof. Background technique [0002] In recent years, with the rapid development of aquaculture in my country and the deepening of intensive aquaculture, it has led to eutrophication in the aquaculture sea area, the degradation of the ecosystem in offshore waters, and the frequent occurrence of various diseases. In order to prevent and treat diseases and improve water quality In the process of aquaculture, a large number of disinfectants, antibiotics and chemical bottom-modifying drugs are often used. While using these drugs, they also inhibit the growth and even death of plankton and beneficial bacteria in the water. It also affects the normal growth of sea cucumbers, fish, shrimps, crabs, etc. It also causes micro-ecological imbalance, and long-term repeated use of certain drugs will cause some pathogen...

AI Technical Summary

Problems solved by technology

It also causes micro-ecological imbalance, and long-term repeated use of certain drugs will cause some pathogenic organisms to develop drug resistance and cause the degradation of the ecological environment. ability

[0003] At present, the microbial bacterial agents produced by our country's bacterial agent factories mainly use Bacillus subtilis, etc., and the strains are single. Due to the backward production technology, the selection of strain medium or carrier is unreasonable, and the number of bacteria and the decomposition ability are low. And affected by environmental factors, the fluctuation is relatively large, and the ability to adapt to the environment is poor

At the same time, solid bacterial agents need to be dissolved when used, which is cumbersome to use and high in cost. Farmers urgently require liquid bacterial agents to be marketed

At present, most of the domestic liquid bacteria are photosynthetic bacteria and yeasts, which are mostly produced by direct filling of fermentation liquid and mineral oil cover technology. The product has a short shelf life (generally about 3 months, and the standard of the Ministry of Agriculture is 6 months), and the quality is not stable. , the product contains only one kind of effective bacteria, when the external environment changes, the vitality of the bacteria will decline and cannot play a role, etc.

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