Culture medium for persimmon tree tissue culture

A tissue culture and culture medium technology, applied in horticulture, botanical equipment and methods, horticultural tools/equipment, etc., can solve the problems of restricting persimmon tree reproduction, unstable survival rate, etc., to shorten the cultivation period, promote growth, genetic good stability

Inactive Publication Date: 2015-06-10
桂林得坤生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the method of breeding seedlings has certain effects, but the survival rate is unstable, which greatly restr

Method used

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  • Culture medium for persimmon tree tissue culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] The medium formula of persimmon tree tissue culture, this medium comprises subculture medium and rooting medium, wherein:

[0060] The components of every liter (L) subculture medium and the content of each component are as follows:

[0061] (1) Macroelements: potassium nitrate 1260mg / L, ammonium nitrate 1320mg / L, calcium chloride dihydrate 440mg / L, magnesium sulfate heptahydrate 370mg / L, potassium dihydrogen phosphate 170mg / L, ferrous sulfate heptahydrate 27.8mg / L, disodium edetate 37.3mg / L;

[0062] (2) Trace elements: manganese sulfate tetrahydrate 22.3mg / L, zinc sulfate heptahydrate 8.6mg / L, boric acid 6.2mg / L, sodium molybdate dihydrate 0.25mg / L, potassium iodide 0.83mg / L, copper sulfate pentahydrate 0.025mg / L, cobalt chloride hexahydrate 0.025mg / L;

[0063] (3) Organic matter: inositol 100mg / L, glycine 2.0mg / L, pyridoxine hydrochloride (VB 6 ) 0.5mg / L, niacin 0.5mg / L, thiamine hydrochloride (VB 1 ) 0.1mg / L, cysteine ​​15mg / L;

[0064] (4) Plant growth regula...

Embodiment 2

[0072] The medium formula of persimmon tree tissue culture, this medium comprises subculture medium and rooting medium, wherein:

[0073] The components of every liter (L) subculture medium and the content of each component are as follows:

[0074] (1) Macroelements: potassium nitrate 1260mg / L, ammonium nitrate 1320mg / L, calcium chloride dihydrate 440mg / L, magnesium sulfate heptahydrate 370mg / L, potassium dihydrogen phosphate 170mg / L, ferrous sulfate heptahydrate 27.8mg / L, disodium edetate 37.3mg / L;

[0075] (2) Trace elements: manganese sulfate tetrahydrate 22.3mg / L, zinc sulfate heptahydrate 8.6mg / L, boric acid 6.2mg / L, sodium molybdate dihydrate 0.25mg / L, potassium iodide 0.83mg / L, copper sulfate pentahydrate 0.025mg / L, cobalt chloride hexahydrate 0.025mg / L;

[0076] (3) Organic matter: inositol 100mg / L, glycine 2.0mg / L, pyridoxine hydrochloride (VB 6 ) 0.5mg / L, niacin 0.5mg / L, thiamine hydrochloride (VB 1 ) 0.1mg / L, cysteine ​​15mg / L;

[0077] (4) Plant growth regula...

Embodiment 3

[0085] The medium formula of persimmon tree tissue culture, this medium comprises subculture medium and rooting medium, wherein:

[0086] The components of every liter (L) subculture medium and the content of each component are as follows:

[0087] (1) Macroelements: potassium nitrate 1260mg / L, ammonium nitrate 1320mg / L, calcium chloride dihydrate 440mg / L, magnesium sulfate heptahydrate 370mg / L, potassium dihydrogen phosphate 170mg / L, ferrous sulfate heptahydrate 27.8mg / L, disodium edetate 37.3mg / L;

[0088] (2) Trace elements: manganese sulfate tetrahydrate 22.3mg / L, zinc sulfate heptahydrate 8.6mg / L, boric acid 6.2mg / L, sodium molybdate dihydrate 0.25mg / L, potassium iodide 0.83mg / L, copper sulfate pentahydrate 0.025mg / L, cobalt chloride hexahydrate 0.025mg / L;

[0089] (3) Organic matter: inositol 100mg / L, glycine 2.0mg / L, pyridoxine hydrochloride (VB 6 ) 0.5mg / L, niacin 0.5mg / L, thiamine hydrochloride (VB 1 ) 0.1mg / L, cysteine ​​15mg / L;

[0090] (4) Plant growth regula...

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PUM

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Abstract

The invention discloses a culture medium for persimmon tree tissue culture. The culture medium comprises a subculture medium and a rooting medium. By adding defined amount of longan juice in the processes of multiplication and rooting culture, the growing of adventitious buds of a persimmon tree can be promoted, the propagation coefficient is high, at the meantime of the multiplication, the rooting is inducted, and the cultivation period is shortened. The rooting percentage and transplanting survival rate are high, and the hereditary stability is good.

Description

technical field [0001] The invention relates to a tissue culture medium, in particular to a persimmon tissue culture medium. Background technique [0002] Persimmon is a temperate deciduous fruit tree of Persimmonaceae berry class. It is native to my country and has a planting history of more than one thousand years in my country. At present, my country has the largest planting area and the largest persimmon fruit output. Persimmons are tender and juicy, sweet and refreshing, and rich in nutrition. There are two kinds of sweet persimmons and astringent persimmons. Astringent persimmons cannot be eaten directly and need to be de-astringent. Sweet persimmons can be eaten fresh. Persimmon is very rich in nutrients, rich in sucrose, glucose, fructose, protein, carotene, vitamin C, citrulline, iodine, calcium, phosphorus, and iron. Fresh persimmon contains high iodine, which can prevent endemic goiter. Persimmon is cold in nature, sweet and astringent in taste, and belongs to...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘小峰何明茜罗知颂李京生刘二伟
Owner 桂林得坤生物科技股份有限公司
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