Unlock instant, AI-driven research and patent intelligence for your innovation.

Immobilization method and application of a kind of salt-resistant and cold-resistant compound bacterial strain

A compound strain and salt-tolerant technology, which is applied in the field of joint degradation of ammonia nitrogen and petroleum hydrocarbons, can solve problems such as not being able to adapt to high salinity environments

Active Publication Date: 2018-01-30
OCEAN UNIV OF CHINA
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Low-temperature oil-degrading bacteria strains, culture methods, culture media and their applications have been studied (ZL201010239621.7). The oil-degrading bacteria screened out by this method have a higher degradation rate than 40%, but cannot adapt to high salinity environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The present invention specifically degrades the polluted Liaohekou wetland water body, and the water quality is: NH 4 -N concentration reaches 35mg / L, NO 2 -N concentration is 12mg / L, NO 3 The concentration of -N is 28mg / L, the concentration of TN is 85mg / L, the concentration of petroleum hydrocarbon is 20mg / L and the salinity is controlled to 10‰. The method of using immobilized salt-tolerant and cold-tolerant mixed bacteria to jointly degrade ammonia nitrogen and petroleum hydrocarbon The shaking treatment under the condition of a temperature of 10°C includes the following steps:

[0033] (1) Preparation of biochar pellets: Weigh 200g of corn stalks, wash with deionized water, dry to constant weight, pulverize the reed stalks with a plant straw pulverizer and pass through a 20-mesh sieve to obtain reed straw powder. Furnace is fired into biochar. The firing conditions of the muffle furnace are: the heating rate is 10℃ / min, the temperature is 300℃ and the holding time is ...

Embodiment 2

[0041] Sampling of a river polluted by ammonia nitrogen and petroleum hydrocarbons, through the detection of NH 4 -N concentration reaches 5.6mg / L, NO 2 — N concentration is 1.9mg / L, NO 3 — The concentration of N is 3.8 mg / L, the concentration of TN is 16.6 mg / L, the concentration of petroleum hydrocarbon is 2.1 mg / L, and the salinity is controlled to 10‰. The immobilized salt-tolerant and cold-tolerant mixed bacteria are used to jointly degrade ammonia nitrogen and petroleum hydrocarbons. The method is to perform shaking treatment at a temperature of 15°C, including the following steps:

[0042] (1) Preparation of biochar balls: Weigh 100g of reed straws, wash them with deionized water, and dry them to a constant weight. Use a plant straw pulverizer to pulverize the reed straws and pass through a 20-mesh sieve to obtain Reed straw powder is burned into biochar through a muffle furnace. The firing conditions of the muffle furnace are: the heating rate is 10℃ / min, the temperatur...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

An immobilization method and application of a salt-resistant and cold-resistant compound bacterial strain. Firstly, high-efficiency anammox bacteria, denitrifying bacteria and oil-degrading bacteria were screened out, and salt tolerance and cold tolerance were domesticated to obtain domesticated Kuenenia.Anamoxidans, Ochrobactrum.sp and Flavobacterium mizutaii respectively. Then put the prepared biochar balls in the enrichment solution of salt-resistant, cold-resistant and high-efficiency composite degrading bacteria, and then mix the biochar balls with the embedding compound solution of salt-resistant, cold-resistant and high-efficiency degrading bacteria, and finally put the biochar balls in the mixture The mixed solution of saturated boric acid solution and 2% CaCl2 is put into the mixed solution one by one, placed in the refrigerator for cross-linking, and washed to obtain immobilized biochar spheres of salt-resistant and cold-resistant composite strains. Applied to the purification of ammonia nitrogen and petroleum hydrocarbon polluted water under low temperature and high salinity conditions, with large microbial biomass. Obviously, the present invention uses biochar spheres as a carrier to immobilize salt-resistant and cold-resistant composite strains. The carrier material has a wide range of sources, a simple preparation method, easy industrialization, high mechanical strength, good treatment effect, low cost, and will not cause damage to the environment. secondary pollution.

Description

Technical field [0001] The invention relates to an immobilization method and application of a salt-tolerant and cold-tolerant composite strain, which specifically utilizes the immobilized anaerobic ammonia oxidation, denitrification and petroleum-degrading bacteria mixed strains to be mixed in a certain proportion to jointly degrade ammonia nitrogen and petroleum under low temperature and high salt conditions hydrocarbon. Background technique [0002] At present, some river water bodies in my country are seriously polluted by ammonia nitrogen and petroleum hydrocarbons, which makes the water bodies smelly and destroys the habitat. The reason why it is difficult to treat natural water bodies polluted by petroleum hydrocarbons and ammonia nitrogen at the same time is that petroleum hydrocarbons inhibit the oxygen enrichment of water bodies, which reduces the activity of aerobic microorganisms or dies. There are physical methods, chemical methods and biological methods commonly use...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/02C12N1/20C02F3/34C12R1/01
Inventor 李岿然黄潇党佳佳李辉田伟君赵阳国白洁陈玲
Owner OCEAN UNIV OF CHINA