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A kind of Hong Kong Sizhaohua tissue culture medium

A Hong Kong four-flowering, tissue culture technology, applied in plant cells, horticulture, botany equipment and methods, etc., can solve the problems of low reproduction efficiency, seedling variation, slow speed, etc., to achieve high reproduction efficiency, high rooting rate, The effect of high multiplication coefficient

Active Publication Date: 2016-06-08
嘉兴卓十生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Seed propagation seedlings in the prior art will produce variation, cannot maintain good properties and seedlings are not neat, affecting the quality of seedlings;
[0009] Although cutting and tillering propagation in the prior art belongs to asexual propagation and can maintain excellent traits, the reproductive efficiency is low and the speed is slow, which is not conducive to the mass propagation and promotion of excellent varieties

Method used

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  • A kind of Hong Kong Sizhaohua tissue culture medium
  • A kind of Hong Kong Sizhaohua tissue culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A Hong Kong Sizhaohua tissue culture medium comprises a primary culture medium, a subculture proliferation medium, a strong seedling medium and a rooting medium. The first-generation culture medium, the subculture proliferation medium, the strong seedling medium and the rooting medium all take the mother medium as the basic medium, and all add plant growth regulators, 20000mg / L of sucrose, and 6500mg / L of carrageenan. L.

[0036] The composition of the mother medium in the primary culture medium is:

[0037] Macroelements: potassium nitrate 3600mg / L, ammonium nitrate 3000mg / L, magnesium sulfate heptahydrate 320mg / L, potassium dihydrogen phosphate 160mg / L, calcium chloride dihydrate 440mg / L.

[0038] Trace elements: potassium iodide 0.8mg / L, manganese sulfate tetrahydrate 22.5mg / L, zinc sulfate heptahydrate 8.6mg / L, boric acid 6mg / L, copper sulfate pentahydrate 0.025mg / L, cobalt chloride 0.025mg / L, Sodium molybdate water 0.25mg / L;

[0039] Iron salt: ferrous sulfate h...

Embodiment 2

[0049] A Hong Kong Sizhaohua tissue culture medium comprises a primary culture medium, a subculture proliferation medium, a strong seedling medium and a rooting medium. The first-generation culture medium, the subculture proliferation medium, the strong seedling medium and the rooting medium all take the mother medium as the basic medium, and all add plant growth regulators, 25000 mg / L of sucrose, and 6000 mg / L of carrageenan. L.

[0050] The composition of the mother medium in the primary culture medium is:

[0051] Macroelements: potassium nitrate 1600mg / L, ammonium nitrate 1400mg / L, magnesium sulfate heptahydrate 300mg / L, potassium dihydrogen phosphate 150mg / L, calcium chloride dihydrate 200mg / L;

[0052] Trace elements: potassium iodide 0.8mg / L, manganese sulfate tetrahydrate 20mg / L, zinc sulfate heptahydrate 8mg / L, boric acid 6mg / L, copper sulfate pentahydrate 0.02mg / L, cobalt chloride 0.02mg / L, molybdenum dihydrate Sodium acid 0.2mg / L;

[0053] Iron salt: ferrous sulf...

Embodiment 3

[0063] A Hong Kong Sizhaohua tissue culture medium comprises a primary culture medium, a subculture proliferation medium, a strong seedling medium and a rooting medium. The first-generation culture medium, the secondary proliferation medium, the strong seedling medium and the rooting medium all take the mother medium as the basic medium, and all add plant growth regulators, 35000mg / L of sucrose, and 7000mg / L of carrageenan. L.

[0064] The composition of the mother medium in the primary culture medium is:

[0065] Macroelements: potassium nitrate 4000mg / L, ammonium nitrate 3200mg / L, magnesium sulfate heptahydrate 340mg / L, potassium dihydrogen phosphate 180mg / L, calcium chloride dihydrate 480mg / L;

[0066] Trace elements: potassium iodide 1mg / L, manganese sulfate tetrahydrate 25mg / L, zinc sulfate heptahydrate 10mg / L, boric acid 7mg / L, copper sulfate pentahydrate 0.03mg / L, cobalt chloride 0.03mg / L, molybdic acid dihydrate Sodium 0.3mg / L;

[0067] Iron salt: ferrous sulfate he...

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Abstract

The invention belongs to the technical field of plant tissue culture and discloses a culture medium for dendrobenthemia japonica tissue culture. The culture medium is characterized in that the culture medium comprises a primary culture medium, a multiplicative culture medium, a strong seedling culture medium and a rooting culture medium which take a mother culture medium as a basal culture medium, and a plant growth regulator, 20000-35000mg / L cane sugar and 6000-7000mg / L carageenan are added to the primary culture medium, the multiplicative culture medium, the strong seedling culture medium and the rooting culture medium. The culture medium has the benefits that 1, the excellent characteristic of a cultured plant is kept; 2, with the adoption of the culture medium for culture, a growth coefficient is high and a rooting rate is high; and 3, with the combination of a culture method, large scale production can be achieved within short time, and the reproduction efficiency is high.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a Hong Kong Sizhaohua tissue culture medium. Background technique [0002] Dendrobenthemia japonica (Dendrobenthemia japonica), also known as litchi and vatica, is an evergreen tree of the genus Dendrobenthemia japonica in the family Cornaceae. Young branches and young leaves sparsely covered with brown fine hairs. Because there are 2 pairs of large yellow-white petal-shaped bracts outside the inflorescence; the fruit is ripe red or purple-red like strawberry and lychee, so it is named mountain lychee. Polyflower fruit spherical red. The drupe is aggregated into a spherical aggregate fruit pulp, matures from September to October, and turns purple after ripening. The fruit tastes sweet and can be eaten and used for wine making. [0003] The tree shape of Hong Kong Sizhaohua is round and umbrella-shaped, with bright leaves and beautiful tree shape. The ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 李波郑国强姜继新余旭峰徐惠芳宋苗
Owner 嘉兴卓十生物科技有限公司