Nutrient solution and method for applying nutrient solution to stem tissue-cultured seedling of dendrobium officinale

A technology of Dendrobium officinale and nutrient solution, applied in the directions of application, horticultural methods, botanical equipment and methods, etc., can solve the problems of uneven quality of seedlings, separation and degradation, and speed up reproduction, and achieve excellent effects, speed up process, and improve reproduction speed. Effect

Inactive Publication Date: 2015-06-24
CROP RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention can maintain the high-quality traits of the female parent, accelerate the reproduction speed, solve the problems of uneven seedlings, separation and degradation in actual production, improve the survival rate and quality of seedlings, and effectively prevent pests and diseases

Method used

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  • Nutrient solution and method for applying nutrient solution to stem tissue-cultured seedling of dendrobium officinale
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  • Nutrient solution and method for applying nutrient solution to stem tissue-cultured seedling of dendrobium officinale

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Protocorm induction and selection of proliferation medium

[0024] After preliminary experiments, the induction rate of the middle part of the stem section was higher than that of the lower and upper part of the stem section. In order to avoid the error between the induction materials, the middle part of the stem section of the same biennial Dendrobium officinale species was selected for the experiment. Firstly, the leaves and leaf sheaths are peeled off, and after being rinsed with clean water, the surface is disinfected with 75% alcohol and 0.1% mercuric chloride solution successively, rinsed with sterile water, and blotted dry with sterile filter paper. Take out the stem section below the bud point by oblique cutting, and inoculate it on protocorm induction and proliferation medium A-E, and inoculate 150 stem sections in each medium:

[0025] Medium A: 1 / 2 MS basic medium, 30% sucrose, 8g / L agar.

[0026] Medium B: 1 / 2 MS basic medium, 0.5mg / L naphthalenea...

Embodiment 2

[0033] Example 2 Cutting method selection

[0034] In order to explore the effects of different cutting methods on the induction of protocorms by stem segments, the middle part of the stem segments of the same biennial Dendrobium officinale species was collected for cutting induction in this experiment. The media for protocorm induction and proliferation were: 1 / 2MS basic medium, 0.5mg / L naphthaleneacetic acid, 1.5mg / L phenylthiadiazolyl urea, 10% taro juice, 30% sucrose and 8g / L agar. Choose the cutting method of A-D as follows to carry out the experiment, and inoculate 150 stem segments in each medium:

[0035] Cutting method A: Carefully scratch the axillary buds of the stem section to stimulate.

[0036]Cutting method B: Take out the lower portion of the adventitious buds and the stem section by longitudinal cutting.

[0037] Cutting method C: Take out the lower portion of the adventitious buds together with the stem section by oblique cutting.

[0038] Table 2 is a tab...

Embodiment 3

[0041] Example 3 Selection of physical stimulation methods

[0042] Collect the middle part of the stem section of the same biennial Dendrobium officinale species for cutting induction, protocorm induction and proliferation medium are: 1 / 2MS basic medium, 0.5mg / L naphthaleneacetic acid, 1.5mg / L phenylthiadiazolyl Urea, 10% taro juice, 30% sucrose and 8g / L agar. Take out the stem section under the adventitious bud by oblique cutting, inoculate 150 stem sections in each medium, and choose the following physical methods A-D for stimulation:

[0043] Stimulation method A: irradiate under ultraviolet light for 1h.

[0044] Stimulation method B: ultrasonic treatment for 15min, 500W.

[0045] Stimulation method C: placed in -8°C environment, cultured in the dark for 5 hours, then transferred to 25°C environment, cultured under light intensity of 2000Lx.

[0046] Stimulation method D: placed in an environment of 4°C, cultured in the dark for 3 hours, microwaved for 2 minutes, power...

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Abstract

The invention provides a nutrient solution and a method for applying the nutrient solution to stem tissue-cultured seedling of dendrobium officinale. One liter of the nutrient solution comprises 500mg of carbamide, 650mg of potassium nitrate, 370mg of calcium nitrate, 590mg of potassium dihydrogen phosphate, 320mg of magnesium sulfate, 85mg of ethylene diamine tetraacetic acid, 60mg of ferrous sulfate, 35mg of manganese sulfate, 20mg of boric acid, 8mg of zinc sulfate, 3.6mg of copper sulfate, 1.9mg of sodium molybdate, 166mg of triacontanol, 26mg of naphthylacetic acid, 42g of chitosan oligosaccharide, 50g of azoxystrobin, leaf ultrasonic liquid and 1L of pure water, wherein the EC value is controlled to be about 0.6; the pH value is adjusted to be 6.0. The nutrient solution is rich in components, has the remarkable effects of promoting the growth of the seedlings and improving the survival rate, and is capable of effectively preventing diseases and insect pests.

Description

technical field [0001] The invention relates to the technical field of tissue culture and planting, in particular to a nutrient solution and an application method thereof in cultivating seedlings of dendrobium officinale stem segments. Background technique [0002] Dendrobium officinale Kimura et Migo is a perennial epiphytic herb of the genus Dendrobium orchidaceae. It has the functions of nourishing yin and kidney, nourishing the lungs and promoting body fluid, anti-tumor, anti-aging, and enhancing the body's immunity. It is a traditional Chinese medicinal material in my country. The Taoist medical classic "Tao Cang" lists Dendrobium candidum as the first of the "Nine Chinese Immortal Herbs", and the folks call it "Life-saving Immortal Herb". [0003] Dendrobium candidum is a unique rare and endangered plant in my country, and it is wildly distributed in Guangdong, Yunnan, Guizhou, Guangxi, Zhejiang, Sichuan and other places in China. It has special requirements on the ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张蕾刘晓津邱道寿胡峰梅瑜
Owner CROP RES INST GUANGDONG ACAD OF AGRI SCI
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