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Micro-nano-scale konjac glucomannan fiber scaffold material preparation method

The technology of konjac glucoside and konjac glucomannan is applied in the preparation of micro-nano-level konjac glucoside fibrous scaffold material, and in the field of biomedical materials, achieving easy processing, large specific surface area and small fiber diameter. Effect

Active Publication Date: 2015-07-15
昆明理工大学设计研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The problem to be solved by the present invention is to solve the existing problems such as low yield of konjac glucosidan electrospinning, quality control of nanofibers, instability of hydrosol, etc.

Method used

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  • Micro-nano-scale konjac glucomannan fiber scaffold material preparation method

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Effect test

Embodiment 1

[0037] The preparation method of the micronano-scale konjac glucosidan fiber scaffold material described in this embodiment specifically includes the following steps:

[0038] (1) Use a graduated cylinder to measure 30ml of distilled water and place it in a 100ml beaker, add 3ml of glycerol and 7ml of glacial acetic acid in turn and stir to fully dissolve to obtain solution A;

[0039] (2) Add 0.8g konjac glucosidan to solution A, and stir well (40min) to obtain semi-thick solution B;

[0040] (3) Filter solution B through a 400-mesh sieve, and vacuum degassing in a vacuum dryer for 6 hours;

[0041] (4) Use a medical syringe to extract 1~3ml of solution, select a stainless steel needle with a 0.07mm aperture, control the ambient temperature to 30°C, adjust the positive voltage to 10Kv, and the receiving distance to 10cm. Such as figure 2 (a)), and finally complete the electrostatic spinning process;

[0042] (5) Dry the sample at room temperature for 24 hours at a vacuum ...

Embodiment 2

[0049] The preparation method of the micronano-scale konjac glucosidan fiber scaffold material described in this embodiment specifically includes the following steps:

[0050] (1) Use a measuring cylinder to measure 30ml of distilled water and place it in a 100ml beaker, add 5ml of ethylene glycol and 5ml of formic acid in turn and stir to fully dissolve to obtain solution A;

[0051] (2) Add 1.7g konjac glucosidan to solution A, stir well (40min) to obtain semi-thick solution B;

[0052] (3) Filter solution B through a 400-mesh sieve, and vacuum degassing in a vacuum dryer for 6 hours;

[0053] (4) Use a medical syringe to extract 1~3ml of solution, select a stainless steel needle with a 0.5mm aperture, control the ambient temperature at 40°C, adjust the positive voltage to 15 Kv, and the receiving distance to 15cm. bent sheet metal, such as figure 2 (b)), and finally complete the electrostatic spinning process;

[0054] (5) Dry the sample at room temperature for 24 hours...

Embodiment 3

[0060] The preparation method of the micronano-scale konjac glucosidan fiber scaffold material described in this embodiment specifically includes the following steps:

[0061] (1) Use a graduated cylinder to measure 30ml of distilled water and place it in a 100ml beaker, add 6ml of propylene glycol and 4ml of formic acid in turn and stir to fully dissolve to obtain solution A;

[0062] (2) Add 1.8g konjac glucosidan to solution A, stir well (40min), and get semi-thick solution B;

[0063] (3) Filter solution B through a 400-mesh sieve, and vacuum degassing in a vacuum dryer for 6 hours;

[0064] (4) Use a medical syringe to draw at least 1~3ml, select a stainless steel needle with an aperture of 1.04mm, control the ambient temperature to 50°C, adjust the positive voltage to 20Kv, and the receiving distance to 20cm. The receiving method is non-uniform plate (needle plate) , and finally complete the electrostatic spinning process;

[0065] (5) Dry the sample at room temperatur...

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Abstract

The present invention discloses a micro-nano-scale konjac glucomannan fiber scaffold material preparation method, and belongs to the field of biomedical material technology application. According to the present invention, the biological material konjac glucomannan with characteristics of good biocompatibility and biodegradability is adopted as the basic raw material and the alcohol / organic acid / water system is adopted as the solvent to prepare the high concentration konjac glucomannan solution, and the electrostatic spinning technology is utilized to prepare the micro-nano-scale konjac glucomannan fiber scaffold in the non-uniform electric field; the preparation method has advantages of simple preparation process, stable product performance, high yield and the like; and the early stage cytotoxicity test results show that: when the concentration of the micro-nano-scale konjac glucomannan fiber scaffold prepared through the method of the present invention is lower than 5 mg / ml, the cytotoxicity standard of the scaffold is at grade 0-1, and no toxicity exists, such that the scaffold has the potential biomedical value.

Description

Technical field: [0001] The invention relates to a preparation method of a micro-nano-level konjac glucosidan fiber scaffold material, which belongs to the technical application field of biomedical materials. Background technique: [0002] Among all the process methods for preparing micro-nanofibrous materials, electrospinning is considered to be the simplest and most effective method. Since the micro-nanofibrous material mimics the structure of the natural extracellular matrix in tissues, it has broad application prospects in the fields of tissue engineering and regenerative medicine. [0003] Konjac glucosidan has many excellent properties, including biocompatibility, degradability, no or low inflammatory response, good physical and mechanical properties, easy processing, abundant sources, mature and simple extraction process, etc. Advantages that natural polymer materials do not have or do not have at the same time. Therefore, its research and application in the field...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/28A61L15/42A61K47/36D04H1/728C12N11/10
Inventor 陈庆华李真颜廷亭冷崇燕
Owner 昆明理工大学设计研究院有限公司
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