Fusobacterium necrophorum selective medium

Abstract

The invention discloses a fusobacterium necrophorum selective medium and a preparation method thereof and belongs to the field of microculture examination. The fusobacterium necrophorum selective medium is characterized in that 1000 ml of culture medium comprises casein tryptone, glucose, yeast powder, sodium chloride, agar, bile salt, ethylene diamine tetraacetic acid dipotassium magnesium salt, gamma-aminobutyric acid, sterile thermal-deformation calf serum and the balance of distilled water. Compared with the prior art, the fusobacterium necrophorum selective medium has the advantages of reliability in examination and high separation rate of fusobacterium necrophorum.

Description

technical field [0001] The invention relates to the field of culture of test microorganisms, in particular to a selective culture medium for fusobacterium necrosis. Background technique [0002] Microbial culture is a technology that uses artificial methods to grow and reproduce microorganisms, and selective medium is a medium designed to promote or inhibit certain types of organisms (such as cells or bacteria, etc.), using this medium can Separation of desired microorganisms from confounding microorganisms. Fusobacterium necrophorum (Fn) is a Gram-negative, strictly anaerobic bacterium of the genus Fusobacterium. Pathogenic Fn strains produce a variety of independent factors, such as leukotoxin (Lkt), hemolysin (hemolysin), hemagglutinin (hemagglutinin) and endotoxin (endotoxin), which can cause multiple mammals (including humans) and Necrobacillosis of poultry. Fusobacterium necroptosis is the main and secondary pathogen of various purulent necrotic infectious diseases,...

AI Technical Summary

Problems solved by technology

[0003] At present, the detection method of Fusobacterium necroptosis mainly adopts the molecular biology detection method based on polymerase chain reaction (PCR). Instruments and equipment are expensive, complicated to operate, and difficult to implement in grassroots laboratories

CDC anaerobic bacteria agar is commonly used in laboratories to cultivate Fusobacterium necrosis. Since most laboratory departments of primary hospitals lack anaerobic testing equipment, most of them use "anaerobic tank culture method". Put the flat plate or liquid medium test tube into the dry tank for cultivation, exhaust the oxygen in the tank by physical and chemical methods, and create an anaerobic environment

The oxygen depletion of the anaerobic cylinder method takes time, and Fusobacterium necroptosis is an obligate anaerobic bacillus, so this culture technique has the following defects: ① Fusobacterium necrosis grows slowly on the CDC anaerobic bacteria agar medium in the anaerobic cylinder , the positive detection rate is low, and a more accurate identification can be made in about a week; ②Because of the non-absolute anaerobic condition, aerobic bacteria and facultative anaerobic bacteria in throat secretion specimens have living conditions, and the separation ability of the medium is poor